Article
Biotechnology & Applied Microbiology
Chen Cheng, Mansoor Chaaban, Gordian Born, Ivan Martin, Qingfeng Li, Dirk J. Schaefer, Claude Jaquiery, Arnaud Scherberich
Summary: The study demonstrates that adipose-based hypertrophic cartilage grafts outperform clinical standard biomaterials in maxillofacial surgery, providing evidence for their effectiveness in restoring mandibular bone tissue.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Yawei Gu, Sebastien Pigeot, Lucas Ahrens, Fabian Tribukait-Riemenschneider, Melika Sarem, Francine Wolf, Andres Garcia-Garcia, Andrea Barbero, Ivan Martin, V. Prasad Shastri
Summary: By developing a bioink system with appropriate rheological properties and using 3D bioprinting technology, it is possible to create high cell density structures of predefined geometry, which can then be implanted into nude mice to generate bone tissue with neovascularization. This method demonstrates the potential for fabricating complex bone tissue for clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Ru-Lin Huang, Rao Fu, Yuxin Yan, Chuanqi Liu, Jing Yang, Yun Xie, Qingfeng Li
Summary: Developmental engineering of hypertrophic cartilaginous constructs engineered from adipose tissue can effectively heal large bone defects, exhibiting superior regenerative capacity and tissue integration.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Andres Pazos-Perez, Maria Pineiro-Ramil, Eloi Franco-Trepat, Maria Guillan-Fresco, Veronica Lopez-Lopez, Alberto Jorge-Mora, Ana Alonso-Perez, Rodolfo Gomez
Summary: The study found that methylphenidate hydrochloride (MPH) may have an impact on the growth plate, leading to premature closure and growth retardation in patients with attention deficit hyperactivity disorder treated with MPH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Xinsheng Zhu, Yong Xu, Xiaoxiong Xu, Junjie Zhu, Linsong Chen, Yawen Xu, Yang Yang, Nan Song
Summary: The use of bevacizumab-laden nanofiber can potentially help regulate the submuscular stability of bone marrow stem cells (BMSCs) engineered cartilage (BEC) to repair tracheal cartilage defects by inhibiting vascular invasion and promoting cartilage maturation.
Review
Engineering, Biomedical
Chang Xie, Jinchun Ye, Renjie Liang, Xudong Yao, Xinyu Wu, Yiwen Koh, Wei Wei, Xianzhu Zhang, Hongwei Ouyang
Summary: This review focuses on strategies of engineered grafts for bone regeneration, emphasizing physicochemical properties and structure optimization to enhance bone regeneration, as well as biological strategies to increase repair efficiency.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
He Zhang, Qian Li, Xiangliang Xu, Siqi Zhang, Yang Chen, Tao Yuan, Ziqian Zeng, Yifei Zhang, Zi Mei, Shuang Yan, Lei Zhang, Shicheng Wei
Summary: In this study, functionalized microscaffold-hydrogel composites were developed to mimic endochondral ossification (ECO) and accelerate bone growth. The microscaffolds enhanced the behavior of human bone marrow mesenchymal stem cells (hBMSCs), while the controlled release of kartogenin induced their differentiation into chondrocytes. The hydrogel, modified with RGD and QK peptides, facilitated chondrocyte hypertrophy and created a vascularized niche for osteogenesis, ultimately accelerating osteochondral repair.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Endocrinology & Metabolism
Nicole Bell, Sanket Bhagat, Shanmugam Muruganandan, Ryunhyung Kim, Kailing Ho, Rachel Pierce, Elena Kozhemyakina, Andrew B. Lassar, Laura Gamer, Vicki Rosen, Andreia M. Ionescu
Summary: This study investigates the role of FoxA factors in chondrocyte differentiation and hypertrophy. The researchers found that overexpression of FoxA2 led to skeletal defects and accumulation of hypertrophic chondrocytes in transgenic mice. However, the ectopic expression of hypertrophic markers was only observed in hypertrophic chondrocytes, suggesting the involvement of other factors like Runx2 in inducing chondrocyte hypertrophy.
Article
Cell Biology
Vanessa Etschmaier, Muammer Uecal, Birgit Lohberger, Markus Absenger-Novak, Dagmar Kolb, Annelie Weinberg, Ute Schaefer
Summary: An ex vivo rat femur slice model was used to investigate the mechanisms of growth impairment following trauma-related bone fractures. The study showed disrupted endochondral ossification, altered expression patterns of specific genes, and increased chondrocyte metabolic activity in the injured area. The presence of stem cells, fibroblasts, and chondrocytes was also observed at the injury site. These findings suggest that the ex vivo organotypic GP injury model is valuable for studying GP regeneration post-trauma and other tissue engineering and disease studies.
Article
Cell Biology
Jonathan C. Bernhard, Darja Marolt Presen, Ming Li, Xavier Monforte, James Ferguson, Gabriele Leinfellner, Patrick Heimel, Susanna L. Betti, Sharon Shu, Andreas H. Teuschl-Woller, Stefan Tangl, Heinz Redl, Gordana Vunjak-Novakovic
Summary: In this study, the effects of endochondral and intramembranous ossification pathways on the properties of engineered bone grafts were evaluated. The results showed that grafts generated through endochondral pathway exhibited better bone regeneration and vascularization, making it more suitable for regenerating large defects. On the other hand, the intramembranous ossification pathway could be used to guide tissue formation with scaffold architecture.
Article
Cell Biology
Encheng Ji, Lieke Leijsten, Janneke Witte-Bouma, Adelin Rouchon, Nunzia Di Maggio, Andrea Banfi, Gerjo J. V. M. van Osch, Eric Farrell, Andrea Lolli
Summary: Tissue engineering bone via endochondral ossification requires the generation of a mineralized cartilage template. Our study investigated the effects of cartilage mineralization on its pro-angiogenic potential. We found that mineralized cartilage showed decreased production of pro-angiogenic factors and reduced ability to stimulate endothelial cell migration, proliferation, and tube formation. This stage-dependent pro-angiogenic potential should be considered in bone tissue engineering strategies.
Article
Biochemistry & Molecular Biology
Vivek Jeyakumar, Nedaa Amraish, Eugenia Niculescu-Morsza, Christoph Bauer, Dieter Pahr, Stefan Nehrer
Summary: Tissue engineering strategies can promote bone regeneration by stimulating osteogenesis route, but the insufficiency of vasculature integration from host tissue remains a challenge. This study demonstrates that a hybrid biomaterial incorporating CD-ECM/SF scaffolds can enhance bone regeneration by priming hBMSC's into the late hypertrophic stage in vitro for ECO-mediated bone tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Angela Fernandez-Iglesias, Rocio Fuente, Helena Gil-Pena, Laura Alonso-Duran, Fernando Santos, Jose Manuel Lopez
Summary: The study investigated the formation of the epiphyseal bone plate using various microscopy techniques and found that the bone tissue is generated through a process different from the usual endochondral ossification. Osteoblasts deposit bone matrix on various surfaces, leading to the establishment of a core of bone sheet and subsequent thickening and reshaping. The processes occur in close relationship with cartilage, providing insight into the complex cellular events underlying bone formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Rao Fu, Chuanqi Liu, Yuxin Yan, Qingfeng Li, Ru-Lin Huang
Summary: Traditional bone tissue engineering strategies face limitations in vascularization and bone regeneration, while engineering cartilaginous constructs mimicking embryonic processes offer a unique ability to promote bone regeneration. These developmentally engineered constructs can serve as transient biomimetic templates for facilitating bone regeneration in critical-sized defects.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Orthopedics
M. Lauwers, A. Courties, J. Sellam, C. Wen
Summary: This review outlines the involvement of the cholinergic system in joint homeostasis and osteoarthritis, including the role of the cholinergic anti-inflammatory pathway, the impact of cholinergic stimulation on chondrocyte proliferation and differentiation, and the regulation of subchondral bone homeostasis by the cholinergic system. The potential of acetylcholinesterase inhibitors as disease-modifying drugs for OA is also discussed.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Engineering, Biomedical
Pierluca Pitacco, Joanna M. Sadowska, Fergal J. O'Brien, Daniel J. Kelly
Summary: This study demonstrates the use of 3D bioprinting to engineer mechanically-reinforced cartilaginous templates for large bone defect regeneration. The study found that hypertrophic grafts engineered using TGF-beta 3 and BMP-2 supported higher levels of blood vessel infiltration and accelerated bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Xavier Barcelo, Kian F. Eichholz, Ines F. Goncalves, Orquidea Garcia, Daniel J. Kelly
Summary: This study demonstrates a biofabrication strategy using inkjet bioprinting to engineer fibrocartilage tissues with user-defined collagen architectures. The scaffold architecture was found to influence the spatial organization of the collagen network, and higher aspect ratios supported the formation of structurally anisotropic tissues. This technology could potentially provide new treatment options for damaged and diseased meniscal tissue.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Selcan Guler, Kian Eichholz, Farhad Chariyev-Prinz, Pierluca Pitacco, Halil Murat Aydin, Daniel J. Kelly, Ibrahim Vargel
Summary: In this study, a biocompatible and bioactive scaffold for bone tissue engineering (BTE) was fabricated using poly(glycerol sebacate) (PGS) and decellularized bone extracellular matrix (deB ECM). The optimized scaffold showed improved initial cell attachment, osteogenesis, mechanical strength, degradation rate, pH change, and wettability. The small-pore-sized and 28% (w/w) bone-inserted scaffolds demonstrated better osteogenic differentiation of mesenchymal stem cells (MSCs).
BIOENGINEERING-BASEL
(2023)
Article
Nanoscience & Nanotechnology
Matthew McGrath, Karolina Zimkowska, Katelyn J. Genoud, Jack Maughan, Javier Gutierrez Gonzalez, Shane Browne, Fergal J. O'Brien
Summary: A biomimetic, bilayered antimicrobial collagen-based scaffold was developed to deal with the etiology of diabetic foot ulcers (DFUs). The scaffold exhibited high structural stability, successfully inhibited the growth and infiltration of Staphylococcus aureus, and supported the proliferation of epidermal cells and vascularization. These results suggest that the bilayered scaffold is a promising candidate for enhancing diabetic wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Lianne C. Shanley, Olwyn R. Mahon, Sinead A. O'Rourke, Nuno G. B. Neto, Michael G. Monaghan, Daniel J. Kelly, Aisling Dunne
Summary: Recent studies have shown that immune cells undergo metabolic reprogramming upon activation, and exploiting this process has therapeutic potential. This study demonstrates for the first time that the size of hydroxyapatite particles can impact macrophage metabolism, with micron-sized particles promoting a pro-inflammatory phenotype through glycolysis. This highlights immunometabolism as a targetable aspect of the immune response for therapeutic benefit and informs intelligent biomaterial design.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Fiona E. Freeman, Pere Dosta, Lianne C. Shanley, Natalia Ramirez Tamez, Cristobal Riojas J. Javelly, Olwyn R. Mahon, Daniel J. Kelly, Natalie Artzi
Summary: This study investigates the potential of miR-29b to suppress osteosarcoma tumors and normalize bone dysregulation caused by the tumor. The delivery of miR-29b along with systemic chemotherapy significantly decreases tumor burden, increases mouse survival, and decreases osteolysis, thereby normalizing bone homeostasis.
ADVANCED MATERIALS
(2023)
Article
Biophysics
Farhad Chariyev-Prinz, Alex Szojka, Nuno Neto, Ross Burdis, Michael G. Monaghan, Daniel J. Kelly
Summary: Mechanical stimulation can modulate the chondrogenic differentiation of stem/progenitor cells, but its effect on functional articular cartilage engineering using human mesenchymal stem cells (hMSCs) may not be necessary if other culture conditions have been optimized. This study investigated the impact of cell seeding densities and fibrin concentrations on chondrogenesis of hMSCs and found that high cell densities were more supportive of sGAG deposition, while lower fibrin concentrations promoted more robust chondrogenesis. Mechanical stimulation had limited effect on cartilage-specific matrix production, except when hMSCs were first primed with TGF-beta 3. These findings suggest that mechanical stimulation may not be essential for engineering functional articular cartilage grafts, but can be used to understand tissue response to mechanical loading in vivo.
JOURNAL OF BIOMECHANICS
(2023)
Article
Engineering, Biomedical
Ian T. Whelan, Ross Burdis, Somayeh Shahreza, Emad Moeendarbary, David A. Hoey, Daniel J. Kelly
Summary: Endochondral ossification (EO) is a critical biological process for bone development and healing. However, understanding of this process is limited, preventing effective treatment of dysregulated EO. The lack of predictive in vitro models is a key factor, and microphysiological systems offer an advanced platform for research. In this study, a microphysiological model of vascular invasion into developing/regenerating bone was developed, mimicking the process of EO. This model successfully replicated key events in EO and could be used for further research and drug testing.
Editorial Material
Engineering, Biomedical
Daniel J. Kelly, Marcy Zenobi-Wong
Article
Veterinary Sciences
Patrycja D. Pakula, Anna Halama, Eman K. Al-Dous, Sarah J. Johnson, Silvio A. Filho, Karsten Suhre, Tatiana Vinardell
Summary: Exercise-induced hemolysis in endurance racehorses was investigated using a metabolite profiling strategy. The study found a significant increase in hemolysis parameters after the race, and a correlation between these parameters, average speed, and distance completed. Levels of hemoglobin degradation metabolites provided a broader insight into the exercise-induced hemolysis process compared to conventional markers.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Nicole van der Vossen, Paulo Cavalcante, Sarah Glynn, Devaya Achappa, Wasiq Mehmood, Masaaki Oikawa, Tatiana Vinardell, Camilla Jamieson
Summary: A previously unreported presentation of guttural pouch empyema (GPE) in a family of juvenile Arabian foals was documented, which showed symptoms of mucopurulent nasal discharge, cough, fever, and tachypnea. The empyema was resolved and associated clinical signs improved with therapeutic guttural pouch lavage and evidence-based antimicrobial use. Cytological examination revealed a neutrophilic exudate with lipid-laden phagocytes, and bacterial culture suggested a high prevalence of opportunistic pathogens including Streptococcus equi ssp. zooepidemicus.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Claudio Intini, Lia Blokpoel Ferreras, Sarah Casey, James E. Dixon, John P. Gleeson, Fergal J. O'Brien
Summary: A novel miR-activated scaffold was developed to enhance mesenchymal stem/stromal cells (MSC) chondrogenesis and cartilage repair through the delivery of an inhibitor to miR-221. The miR-activated scaffold successfully transfected human MSCs with the miR-221 cargo, promoting an improved cell-mediated chondrogenic response. This innovative scaffold shows promise in improving chondrogenesis and enhancing cartilage defect repair.
ADVANCED THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Piotr Gierlich, Claire Donohoe, Kevin Behan, Daniel J. Kelly, Mathias O. Senge, Ligia C. Gomes-da-Silva
Summary: A pH-sensitive hydrogel formulation was developed for intratumoral injection, enabling sustained release of photosensitizers and achieving optimal therapeutic outcomes through multiple light exposures.
Review
Urology & Nephrology
Sorcha O'Meara, Eoghan M. Cunnane, Stefanie M. Croghan, Connor V. Cunnane, Michael T. Walsh, Fergal J. O'Brien, Niall F. Davis
Summary: The ureter is a complex structure with varying mechanical properties and morphology along its anatomical length. Understanding the mechanical forces and properties of the ureter is important for surgical outcomes and urine transport. Further studies on human ureteric tissue are needed to better understand these properties and the influence of forces on them.
NATURE REVIEWS UROLOGY
(2023)
Article
Engineering, Biomedical
Kian F. Eichholz, Pierluca Pitacco, Ross Burdis, Farhad Chariyev-Prinz, Xavier Barcelo, Brooke Tornifoglio, Ryan Paetzold, Orquidea Garcia, Daniel J. Kelly
Summary: This study demonstrates the potential of integrating different additive manufacturing approaches, specifically MEW and FDM, for the development of regenerative biomaterials. By leveraging the advantages of these two methods, hybrid MEW-FDM constructs are able to achieve enhanced bone healing outcomes.
ADVANCED HEALTHCARE MATERIALS
(2023)
