Article
Cell Biology
Malgorzata Tokarska-Schlattner, Laurence Kay, Pascale Perret, Raffaella Isola, Stephane Attia, Frederic Lamarche, Cindy Tellier, Cecile Cottet-Rousselle, Amjad Uneisi, Isabelle Hininger-Favier, Marc Foretz, Herve Dubouchaud, Catherine Ghezzi, Christian Zuppinger, Benoit Viollet, Uwe Schlattner
Summary: AMPK, a key regulator of energy homeostasis, plays important roles in maintaining cardiac function under increased workload conditions. Deletion of AMPK genes in cardiomyocytes did not affect heart function under physiological conditions, but led to alterations in response to increased workload. AMPK deletion also resulted in decreased basal metabolic rate and locomotor activity, along with changes in cardiac mitochondrial function and bioenergetics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Zhiming Wang, Jing Tan, Mengxuan Li, Can Gao, Wenwen Li, Jing Xu, Changchuan Guo, Zhenzhen Chen, Rong Cai
Summary: ATP is essential for energy transfer in organisms, and kinases play a crucial role in cell signaling. Small molecule kinase inhibitors have been used to treat diseases, but their selectivity needs to be evaluated. In this study, a clickable ATP photoaffinity probe was developed to identify ATP-binding proteins, specifically kinases. This method was also used to analyze the targets of selected kinase inhibitors. The study demonstrated that this chemoproteomic approach is effective for high-throughput target identification of kinase inhibitors.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wan Sun, Xiangling Gu, Pingxuan Dong, Lianjun Chu, Zhongyu Zhang, Zhenyuan Cheng, Fan Yang
Summary: In this study, a membrane-targeted near-infrared small molecule fluorescent probe was developed for the detection of extracellular ATP. This probe showed high sensitivity and specificity, enabling quantitative detection of extracellular ATP in tumor diagnosis and treatment.
Article
Gastroenterology & Hepatology
Gangarao Davuluri, Nicole Welch, Jinendiran Sekar, Mahesha Gangadhariah, Khaled Alsabbagh Alchirazi, Maradumane L. Mohan, Avinash Kumar, Sashi Kant, Samjhana Thapaliya, McKenzie Stine, Megan R. McMullen, Rebecca L. McCullough, George R. Stark, Laura E. Nagy, Sathyamangla V. Naga Prasad, Srinivasan Dasarathy
Summary: Despite the high clinical significance of sarcopenia in alcohol-associated cirrhosis, the mechanisms of ethanol-induced impaired protein homeostasis are poorly understood. This study identified PP2A as a key mediator in ethanol-induced dysregulation of protein balance, suggesting potential therapeutic approaches targeting the PI3K gamma-PP2A axis.
Article
Chemistry, Multidisciplinary
Tie-Gang Meng, Jia-Ni Guo, Liu Zhu, Yike Yin, Feng Wang, Zhi-Ming Han, Lei Lei, Xue-Shan Ma, Yue Xue, Wei Yue, Xiao-Qing Nie, Zheng-Hui Zhao, Hong-Yong Zhang, Si-Min Sun, Ying-Chun Ouyang, Yi Hou, Heide Schatten, Zhenyu Ju, Xiang-Hong Ou, Zhen-Bo Wang, Catherine C. L. Wong, Zhonghan Li, Qing-Yuan Sun
Summary: The study reveals that NLRP14 is crucial for maintaining calcium oscillations and early embryonic development. Disruption of NLRP14 leads to impaired cytoplasmic function and calcium homeostasis due to altered mitochondrial distribution, morphology, and activity. The interaction between NLRP14 and the mitochondrial Na+/Ca2+ exchanger (NCLX) is essential for regulating calcium homeostasis and embryonic development.
Article
Cell Biology
Hassan Akbari, Farzad Taghizadeh-Hesary, Moslem Bahadori
Summary: This article provides an overview of the potential predictive factors of PD-1 blockade in oncology and emphasizes the crucial role of mitochondria in response to anti-PD 1 therapies.
Review
Biochemistry & Molecular Biology
Fiona M. Russell, David Grahame Hardie
Summary: AMP-activated protein kinase (AMPK) plays a crucial role in regulating cellular energy balance by promoting ATP production and inhibiting cell growth. It may restrain aberrant growth before tumorigenesis, but support the survival of cancer cells once cancer has arisen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Chunlin Chen, Bin Wang, Guoqing Xiao, Min Cao, Fei Zhong, Jian Zhou, Zhengwei Yang, Mingtan Wang, Rui Zou
Summary: Carbon spheres were coated with polydopamine and adenosine triphosphate to obtain activated carbon spheres with C-N-P structure, which contributed to the formation of intumescent char layer. Zinc hydroxystannate was introduced to the surface of carbon phosphide spheres to create a composite functional hybrid reinforcement. The CS/PAP@ZHS-based EP exhibited the best fire and smoke suppression properties.
PROGRESS IN ORGANIC COATINGS
(2023)
Review
Biochemistry & Molecular Biology
T. B. Beena, Mathew. A. Jesil, K. B. Harikumar
Summary: The major cause of colorectal cancer (CRC) related mortality is its metastasis. Signaling pathways, such as the sonic hedgehog (Shh) pathway, and AMP-activated protein kinase (AMPK) play important roles in CRC development and progression. This review explores the interconnection between high-fat diet consumption, Shh pathway activation, and the progression of CRC, as well as the potential regulatory role of AMPK in controlling CRC complexity and lipid levels.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Veterinary Sciences
Xu-Yang Gao, Bu-Hao Deng, Xin-Rui Li, Yu Wang, Jian-Xin Zhang, Xiao-Yan Hao, Jun-Xing Zhao
Summary: The study found that melatonin enhances the formation of sheep brown adipocytes by activating AMPKα1, reducing lamb mortality and economic losses.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Rachel Aruldas, Laura Buczek Orenstein, Sade Spencer
Summary: Repeated cocaine exposure induces changes in AMPK and pAMPK in different brain regions and cellular compartments, with some differences between acute withdrawal and ongoing cocaine treatment. Pretreatment with metformin, an indirect AMPK activator, enhances acute cocaine-induced locomotor activity but blocks the development of sensitization. These findings provide insights into the dynamics of AMPK activation in subcellular compartments and support the potential use of metformin for the treatment of cocaine use disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ben Zhou, Yuyao Zhang, Sainan Li, Lianfeng Wu, Geza Fejes-Toth, Aniko Naray-Fejes-Toth, Alexander A. Soukas
Summary: SGK1 plays a crucial role in hepatic insulin resistance in type 2 diabetes, affecting AMP-activated protein kinase (AMPK). Liver-specific Sgk1 knockout mice show improved glucose tolerance and insulin sensitivity, suggesting therapeutic potential in targeting hepatic SGK1.
Review
Cell Biology
Julieta Schmidt, Hector Ramiro Quinta
Summary: Traumatic spinal cord injuries cause disruption of axonal projections, resulting in impaired locomotor, sensory, and autonomic functions. Mitochondrial dysfunction in the injured spinal tissue prevents the regeneration of severed axons, leading to sustained impairment in voluntary control of movement. This review discusses the link between mitochondrial dysfunction and axonal regeneration failure in spinal cord injury, and explores potential mitochondria-targeted therapeutic approaches to promote functional recovery.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Chuanqi Sun, Zonglin Li, Xiao Ning, Wentian Xu, Zhimin Li
Summary: In this study, an in vitro multi-enzyme cascade system was established for the regeneration and production of ATP, utilizing adenosine and polyP as key substrates. Two functionally distinct PPKs were selected for high rates of ATP production, with Sulfurovum lithotrophicum PPK exhibiting excellent activity and stability over a wide pH range. After optimizing the reaction conditions, ATP yields as high as 76.0% were achieved with different initial adenosine concentrations, demonstrating the feasibility and efficiency of the system.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Environmental Sciences
Zhenjie Sun, Wenhui Gu, Zezhong Feng, Yaqin Fan, Jianfeng Niu, Guangce Wang
Summary: This study elucidated the expression regulation mechanism of the heat shock protein 70 gene in Neopyropia yezoensis under high temperature stress. The findings showed the involvement of cAMP-dependent protein kinase and activating transcription factors element in the transcription regulation of the gene. In addition, AMPK was also found to play a role in the induction of the gene. Furthermore, posttranscriptional mechanisms were proposed to be involved in the synthesis regulation of the heat shock protein 70-2 protein.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Cell Biology
D. Grahame Hardie
CURRENT OPINION IN CELL BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Madhumita Dandapani, D. Grahame Hardie
BIOCHEMICAL SOCIETY TRANSACTIONS
(2013)
Article
Endocrinology & Metabolism
D. Grahame Hardie
Article
Biochemistry & Molecular Biology
Morgan D. Fullerton, Sandra Galic, Katarina Marcinko, Sarah Sikkema, Thomas Pulinilkunnil, Zhi-Ping Chen, Hayley M. O'Neill, Rebecca J. Ford, Rengasamy Palanivel, Matthew O'Brien, D. Grahame Hardie, S. Lance Macaulay, Jonathan D. Schertzer, Jason R. B. Dyck, Bryce J. van Denderen, Bruce E. Kemp, Gregory R. Steinberg
Review
Endocrinology & Metabolism
Gregory R. Steinberg, Madhumita Dandapani, D. Grahame Hardie
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2013)
Article
Biochemistry & Molecular Biology
Javier Moral-Sanz, Sophronia A. Lewis, Sandy Macmillan, Fiona A. Ross, Adrian Thomson, Benoit Viollet, Marc Foretz, Carmel Moran, D. Grahame Hardie, A. Mark Evans
Review
Biochemistry & Molecular Biology
Diana Vara-Ciruelos, Fiona M. Russell, D. Grahame Hardie
Review
Biochemistry & Molecular Biology
A. Mark Evans, D. Grahame Hardie
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Fiona M. Russell, David Grahame Hardie
Summary: AMP-activated protein kinase (AMPK) plays a crucial role in regulating cellular energy balance by promoting ATP production and inhibiting cell growth. It may restrain aberrant growth before tumorigenesis, but support the survival of cancer cells once cancer has arisen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Correction
Biology
Sandy MacMillan, Andrew P. Holmes, Mark L. Dallas, Amira D. Mahmoud, Michael J. Shipston, Chris Peers, D. Grahame Hardie, Prem Kumar, A. Mark Evans
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Sandy MacMillan, Andrew P. Holmes, Mark L. Dallas, Amira D. Mahmoud, Michael J. Shipston, Chris Peers, D. Grahame Hardie, Prem Kumar, A. Mark Evans
Summary: This study reveals that LBK1 is a critical regulator of carotid body chemosensing, highlighting a difference in dependency on LKB1 and AMPK between the carotid body and the hypoxic ventilatory response.
COMMUNICATIONS BIOLOGY
(2022)
Review
Cell Biology
Gregory R. Steinberg, D. Grahame Hardie
Summary: AMP-activated protein kinase (AMPK) is a cellular energy sensor that is activated by increases in AMP/ADP to ATP ratios. Recent studies have discovered non-canonical pathways for AMPK activation, involving calcium, nutrients, metabolites, DNA and lysosome damage. AMPK has been found to regulate carbohydrate and lipid metabolism, mitochondrial and lysosomal homeostasis, and DNA repair. Its role in cancer, obesity, diabetes, nonalcoholic steatohepatitis (NASH), and other disorders has been explored, suggesting potential therapeutic targets.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
D. Grahame Hardie
Summary: This article reflects on the author's personal research journey, focusing on the early stages of defining AMPK and developing related tools, the exciting period of identifying upstream kinases in the late 1990s and early 2000s, and recent research on the complex role of AMPK in cancer.
BIOCHEMICAL JOURNAL
(2022)
Review
Oncology
D. Grahame Hardie
Summary: Otto Warburg published the first paper on the Warburg effect in 1923, revealing the difference in lactate production between tumor tissues and normal tissues in glucose metabolism. However, the mechanisms underlying the Warburg effect are still not fully understood to this day.
ENDOCRINE-RELATED CANCER
(2022)