期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 140, 期 5, 页码 1767-1773出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b11014
关键词
-
资金
- AMED-CREST
- JST/PRESTO [JPMJPR14F8]
- MEXT/JSPS KAKENHI [JP16H02606, JP26111012, 16H06574]
- Resonance Bio [JP15H0S9S1]
- JSPS Core-to-Core Program, A, Advanced Research Networks
- Project for Cancer Research And Therapeutic Evolution (P-CREATE) from the Japan Agency for Medical Research and development, AMED
- Hoansha Foundation
- Japan Foundation for Applied Enzymology
- [16J09996]
- Grants-in-Aid for Scientific Research [26111012, 16H05099, 17K19477, 16J09996, 15H05371, 15H05951, 16H06574, 16H02606, 17J10083] Funding Source: KAKEN
Carboxypeptidases (CPs) are a family of hydrolases that cleave one or more amino acids from the C-terminal of peptides or proteins. However, methodology to monitor the activities of CPs is poorly developed. Here, we present the first versatile design strategy to obtain activatable fluorescent probes for CPs by utilizing intramolecular spirocyclization of rhodamine to translate the aliphatic carboxamide to aliphatic carboxylate structural conversion catalyzed by CPs into dynamic fluorescence activation. Based on this novel strategy, we developed probes for carboxypeptidases A and B. One of these probes was able to detect pancreatic juice leakage in mice ex vivo, suggesting that its suitability for intraoperative diagnosis of pancreatic fistula. This design strategy should be broadly applicable to CPs, as well as other previously untargetable enzymes, enabling development of fluorescent probes to study various pathological and biological processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据