期刊
JOURNAL OF REPRODUCTION AND DEVELOPMENT
卷 64, 期 1, 页码 49-55出版社
SOCIETY REPRODUCTION & DEVELOPMENT-SRD
DOI: 10.1262/jrd.2017-042
关键词
Brusatol; Cell cycle; Early embryo; Nrf2
资金
- Foundation of Jinling Hospital, Clinical School of Medical College, Nanjing University [2014027, 2014030]
- National Natural Science Foundation of China [81503655]
Brusatol, a quassinoid isolated from the fruit of Bruceajavanica, has recently been shown to inhibit nuclear factor erythroid 2-related factor 2 (Nrf2) via Keapl-dependent ubiquitination and proteasomal degradation or protein synthesis. Nrf2 is a transcription factor that regulates the cellular defense response. Most studies have focused on the effects of Nrf2 in tumor development. Here, the critical roles of Nrf2 in mouse early embryonic development were investigated. We found that brusatol treatment at the zygotic stage prevented the early embryo development. Most embryos stayed at the two-cell stage after 5 days of culture (P < 0.05). This effect was associated with the cell cycle arrest, as the mRNA level of CDK1 and cyclin B decreased at the two-cell stage after brusatol treatment. The embryo development potency was partially rescued by the injection of Nrf2 CRISPR activation plasmid. Thus, brusatol inhibited early embryo development by affecting Nrf2-related cell cycle transition from G(2) to M phase that is dependent on cyclin B-CDK1 complex.
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