期刊
JOURNAL OF PROTEOME RESEARCH
卷 17, 期 8, 页码 2735-2743出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.8b00190
关键词
metabolomics; equine; synovial fluid; osteoarthritis; osteochondrosis; sepsis; nuclear magnetic resonance
资金
- Veterinary School Research Project Support Grant [VSRP VET016]
- Technology Directorate voucher scheme, University of Liverpool
- Wellcome Trust Intermediate Clinical Fellowship [107471/Z/15/Z]
- Horse Trust PhD studentship [G1015]
- MRC Clinical Research Capabilities and Technologies Initiative [MR/M009114/1]
- MRC [MR/M009114/1, MR/P020941/1] Funding Source: UKRI
Osteoarthritis (OA), osteochondrosis (OC), and synovial sepsis in horses cause loss of function and pain. Reliable biomarkers are required to achieve accurate and rapid diagnosis, with synovial fluid (SF) holding a unique source of biochemical information. Nuclear magnetic resonance (NMR) spectroscopy allows global metabolite analysis of a small volume of SF, with minimal sample preprocessing using a noninvasive and nondestructive method. Equine SF metabolic profiles from both nonseptic joints (OA and OC) and septic joints were analyzed using 1D H-1 NMR spectroscopy. Univariate and multivariate statistical analyses were used to identify differential metabolite abundance between groups. Metabolites were annotated via H-1 NMR using 1D NMR identification software Chenomx, with identities confirmed using 1D H-1 and 2D H-1 C-13 NMR. Multivariate analysis identified separation between septic and nonseptic groups. Acetate, alanine, citrate, creatine phosphate, creatinine, glucose, glutamate, glutamine, glycine, phenylalanine, pyruvate, and valine were higher in the nonseptic group, while glycylproline was higher in sepsis. Multivariate separation was primarily driven by glucose; however, partial-least-squares discriminant analysis plots with glucose excluded demonstrated the remaining metabolites were still able to discriminate the groups. This study demonstrates that a panel of synovial metabolites can distinguish between septic and nonseptic equine SF, with glucose the principal discriminator.
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