Article
Pharmacology & Pharmacy
Katharina Held, Vincenzo Davide Aloi, Ana Cristina Nogueira Freitas, Annelies Janssens, Andrei Segal, Julia Przibilla, Stephan Ernst Philipp, Yu Tian Wang, Thomas Voets, Joris Vriens
Summary: This study compares the pharmacological properties of different splice variants of TRPM3, demonstrating that the length of the pore-forming loop in the alternative splicing region defines the channel's activation by specific compounds.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Anesthesiology
Vincenzo Davide Aloi, Silvia Joao Poseiro Coutinho Pinto, Rita Van Bree, Katrien Luyten, Thomas Voets, Joris Vriens
Summary: Chemotherapy-induced peripheral neuropathic pain (CIPNP) is a common adverse effect in cancer patients receiving cytostatic drugs. Current treatment options for CIPNP are limited and unsatisfactory. This study suggests that TRPM3 could be a potential target for the treatment of neuropathic pain in chemotherapy patients.
Article
Pharmacology & Pharmacy
Balazs Kelemen, Silvia Pinto, Nawoo Kim, Erika Lisztes, Martin Hanyicska, Anita Vladar, Attila Olah, Zsofia Penzes, Brian Shu, Joris Vriens, Tamas Biro, Tibor Rohacs, Thomas Voets, Balazs Istvan Toth
Summary: TRPM3 selectively mediates pain sensation but not itch sensation, making it a promising candidate for selectively targeting pain sensation.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biology
Stephanie W. Tam, Kate Huffer, Mufeng Li, Kenton J. Swartz
Summary: P2X receptor channels are trimeric ATP-activated ion channels expressed in neuronal and non-neuronal cells. Seven subtypes have been identified in mammals that can form both homomeric and heteromeric channels. The recent study demonstrates that ions can enter or exit the internal pore through lateral fenestrations, which play a critical role in determining the ion selectivity of P2X receptor channels.
Article
Biochemistry & Molecular Biology
Yuefang Zhou, Thomas M. Bennett, Alan Shiels
Summary: TRPM3 gene mutation is associated with early-onset cataracts, and our study on mice with this mutation provides insights into ocular phenotypes.
Review
Cell Biology
Katharina Held, Balazs Istvan Toth
Summary: Ion channels play a crucial role in regulating neural processes in the brain, and disruptions in their functions can lead to various neurological disorders. TRP ion channels are key players in the development and physiological functions of the central nervous system, influencing processes like neurite growth and synaptic plasticity. TRPM3, a non-selective cation channel, is emerging as a new player in the brain with potential roles in neuropathological processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Siyuan Zhao, Tibor Rohacs
Summary: TRPM3 is a Ca2+ permeable nonselective cation channel activated by heat and chemical agonists. It plays a critical role in noxious heat sensation in mice, while its role in the human brain remains largely unexplored. Mutations in TRPM3 have been associated with developmental and epileptic encephalopathy, indicating its important role in the human brain.
Article
Agriculture, Dairy & Animal Science
Karina Lezama-Garcia, Daniel Mota-Rojas, Alfredo M. F. Pereira, Julio Martinez-Burnes, Marcelo Ghezzi, Adriana Dominguez, Jocelyn Gomez, Ana de Mira Geraldo, Pamela Lendez, Ismael Hernandez-Avalos, Isabel Falcon, Adriana Olmos-Hernandez, Dehua Wang
Summary: This review discusses recent discoveries about transient receptor potential and their role in thermoregulatory mechanisms of animals, emphasizing the importance of understanding how these receptors are activated and the pathways they use to recognize specific thermal sensations. Ongoing research aims to develop therapeutic approaches targeting these receptors to improve non-human animals' quality of life. The review presents and analyzes findings on TRP structure, physiology, and neurotransmission in thermoregulation, highlighting the need for further studies to advance our understanding of these mechanisms and control thermoregulatory disorders.
Article
Chemistry, Physical
Hui-Yuan Zhang, Qin Xu, Dong-Qing Wei
Summary: Charge plays a key role in passive permeation of small molecules, and simulations on water and sulfide molecules reveal distinct characteristics in their permeation processes, with free energy highly correlated with the hydrophilic shell.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Joanna K. Bujak, Daria Kosmala, Kinga Majchrzak-Kuligowska, Piotr Bednarczyk
Summary: The study confirmed the expression of the TRPV1 ion channel on both mRNA and protein levels in canine PBMC, and indicated that the ion channel is functional.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Alvaro Otero-Sobrino, Pablo Blanco-Carlon, Miguel Angel Navarro-Aguadero, Miguel Gallardo, Joaquin Martinez-Lopez, Maria Velasco-Estevez
Summary: Mechanosensitive ion channels play a crucial role in sensing mechanical changes and regulating cellular functions, with significant implications for cancer research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Joshua M. Thurman, Veronique Fremeaux-Bacchi
Summary: Uncontrolled alternative pathway activation plays a crucial role in the development of various diseases and contributes to their pathogenesis. Therefore, the development and use of diagnostic tests to monitor the alternative pathway is of increasing importance. These tests assess the function, expression, and activation fragments of the alternative pathway. Genetic studies have identified disease-associated variants in alternative pathway genes that can predict disease risk and prognosis. Additionally, new imaging modalities offer non-invasive detection and localization of pathologic complement activation.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Engineering, Biomedical
S. Kozaka, R. Wakabayashi, N. Kamiya, M. Goto
Summary: Transcutaneous drug delivery is a promising method for drug repositioning and reformulation due to its non-invasive and easy-to-use features. By using a gel-like lyotropic liquid crystal (LLC), this study demonstrated that adjusting the liquid crystalline structure can enhance skin permeation of peptide drugs and show a strong vaccine effect in vivo, providing a basis for designing transcutaneous delivery systems of peptide drugs with LLC.
ACTA BIOMATERIALIA
(2022)
Editorial Material
Biochemistry & Molecular Biology
Elena O. Gracheva, Sviatoslav N. Bagriantsev
Summary: David Julius and Ardem Patapoutian were awarded this year's Nobel Prize in Physiology or Medicine for explaining the molecular basis for sensing heat, cold and mechanical force, marking the end of a scientific journey to identify the mechanisms in the somatosensory system mediating the detection of internal and external environments.
Review
Cell Biology
Giorgia Chinigo, Helene Castel, Oana Chever, Dimitra Gkika
Summary: Malignant glioma, especially glioblastoma (GBM), is a common primary brain tumor with high resistance to standard treatment. Recent studies have identified TRP channels as potential new molecular targets for glioma treatment, offering directions for innovative strategies to overcome high resistance and recurrence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Katharina Held, Vincenzo Davide Aloi, Ana Cristina Nogueira Freitas, Annelies Janssens, Andrei Segal, Julia Przibilla, Stephan Ernst Philipp, Yu Tian Wang, Thomas Voets, Joris Vriens
Summary: This study compares the pharmacological properties of different splice variants of TRPM3, demonstrating that the length of the pore-forming loop in the alternative splicing region defines the channel's activation by specific compounds.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Neurosciences
Maya O. Nesbit, Anping Chai, Peter Axerio-Cilies, Anthony G. Phillips, Yu Tian Wang, Katharina Held
Summary: The widely used dopamine D-1 receptor agonist SKF81297 can modulate neurotransmission by potentiating glutamatergic NMDAR currents independently of D1R activation. These findings have important implications for interpreting studies on dopamine functions that rely on these compounds.
Article
Biology
Helene De Bruyn, Nikky Corthout, Sebastian Munck, Wouter Everaerts, Thomas Voets
Summary: Understanding the lower urinary tract (LUT) is crucial for developing novel therapies for LUT disorders. We developed a videocystometry technique to accurately monitor the LUT in rodents, but it had limitations in awake and freely moving animals. To overcome this, we used machine learning to automatically analyze bladder filling and voiding cycles in behaving mice. Our findings revealed the effects of anesthesia on urethral relaxation and voiding duration, as well as the decreased bladder capacity after surgical implantation of a catheter. This approach provides a paradigm for noninvasive, in vivo monitoring of hollow organs in behaving animals and highlights the limitations of current standard techniques for studying the LUT in mice.
Article
Obstetrics & Gynecology
Brecht Geysenbergh, Anne-Sophie Boes, Celine Bafort, Anne-Sophie van Rompuy, Sara Neyens, Sharon Lie Fong, Sophie Debrock, Joris Vriens, Peter De Loecker, Eline Dancet, Thomas D'Hooghe, Karen Peeraer
Summary: This study aimed to investigate the prevalence of chronic endometritis (CE) in infertile women, its impact on reproductive outcomes, and the accuracy of hysteroscopy as a screening tool for CE. The study included 514 asymptomatic patients with infertility who underwent hysteroscopy and endometrial biopsy (EMB). CE was identified in 2.8% of patients starting assisted reproductive technologies (ART). The clinical pregnancy rate (CPR) did not differ significantly between patients with CE and the entire cohort of patients without CE, but was significantly lower in the 2 years after EMB in patients with CE compared to matched controls. Hysteroscopy had low sensitivity and high specificity in diagnosing CE.
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
(2023)
Article
Biology
Lydie Burglen, Evelien Van Hoeymissen, Leila Qebibo, Magalie Barth, Newell Belnap, Felix Boschann, Christel Depienne, Katrien De Clercq, Andrew G. L. Douglas, Mark P. Fitzgerald, Nicola Foulds, Catherine Garel, Ingo Helbig, Katharina Held, Denise Horn, Annelies Janssen, Angela M. Kaindl, Vinodh Narayanan, Christina Prager, Mailys Rupin-Mas, Alexandra Afenjar, Siyuan Zhao, Vincent Th Ramaekers, Sarah M. Ruggiero, Simon Thomas, Stephanie Valence, Lionel Van Maldergem, Tibor Rohacs, Diana Rodriguez, David Dyment, Thomas Voets, Joris Vriens
Summary: TRPM3 is a plasma membrane cation channel that is sensitive to temperature and neurosteroids and is expressed in various types of cells. Rare variants in TRPM3 have been found in individuals with developmental and epileptic encephalopathy, but the connection between TRPM3 activity and neurological disorders is not well understood. In this study, we identified additional heterozygous missense variants in TRPM3 in 10 patients with a range of neurodevelopmental symptoms. These variants cause a gain-of-function phenotype, leading to increased channel activity and altered calcium levels in cells. Treatment with the TRPM3 antagonist primidone reduced the increased channel activity, suggesting that TRPM3 antagonists could be a potential therapy for these disorders.
Article
Pharmacology & Pharmacy
Jan Daniluk, Thomas Voets
Summary: TRPV1 antagonists have pH-dependent effects on channel inhibition, but their modality selectivity is not strict. The antagonistic efficacy of TRPV1 antagonists is limited at acidic pH, which may restrict their analgesic efficacy in injured tissues.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Clinical Neurology
Lena-Luise Becker, Denise Horn, Felix Boschann, Evelien Van Hoeymissen, Thomas Voets, Joris Vriens, Christine Prager, Angela M. Kaindl
Summary: DEE-SWAS is an age-dependent disease that causes cognitive decline. This study reports on two children with DEE-SWAS and developmental delay/regression, who had missense variants in the TRPM3 gene. Treatment with primidone improved their developmental regression, psychomotor development, and eliminated CSWS.
Article
Pharmacology & Pharmacy
Noemie Luyts, Jan Daniluk, Ana Cristina Nogueira Freitas, Bahar Bazeli, Annelies Janssens, Marie Mulier, Wouter Everaerts, Thomas Voets
Summary: In this study, the effect of Phenazopyridine (PAP) on pain-related Transient Receptor Potential (TRP) channels was investigated. The results showed that PAP can rapidly and reversibly inhibit the cold and menthol sensitivity of TRPM8 channels. This suggests that the inhibition of TRPM8 may be the mechanism underlying the analgesic activity of PAP.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Anesthesiology
Vincenzo Davide Aloi, Silvia Joao Poseiro Coutinho Pinto, Rita Van Bree, Katrien Luyten, Thomas Voets, Joris Vriens
Summary: Chemotherapy-induced peripheral neuropathic pain (CIPNP) is a common adverse effect in cancer patients receiving cytostatic drugs. Current treatment options for CIPNP are limited and unsatisfactory. This study suggests that TRPM3 could be a potential target for the treatment of neuropathic pain in chemotherapy patients.