期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 4, 页码 2183-2188出版社
AMER CHEMICAL SOC
DOI: 10.1021/am508292t
关键词
microfluidics; 3D cell culture; aligned hydrogel; shear flow; ECM
资金
- Global Ph.D Fellowship Program [2011-006423]
- Basic Science Research Program [2013R1A1A2006148]
- Brain Research Program [2012M3C7A1055413]
- Biomembrane Plasticity Research Center through the National Research Foundation of Korea (NRF) [2014051826]
We report a microfluidic approach to impart alignment in ECM components in 3D hydrogels by continuously applying fluid flow across the bulk gel during the gelation process. The microfluidic device where each channel can be independently filled was tilted at 90 degrees to generate continuous flow across the Matrigel as it gelled. The presence of flow helped that more than 70% of ECM components were oriented along the direction of flow, compared with randomly cross-linked Matrigel. Following the oriented ECM components, primary rat cortical neurons and mouse neural stem cells showed oriented outgrowth of neuronal processes within the 3D Matrigel matrix.
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