期刊
JOURNAL OF PHARMACY AND PHARMACOLOGY
卷 70, 期 3, 页码 413-425出版社
WILEY
DOI: 10.1111/jphp.12872
关键词
clover; coagulation; cytotoxicity; fibrin; thrombin
资金
- University of Lodz, Poland [506/1136]
- European Community, OSCAR [FP7-KBBE-2011-5-289277]
ObjectiveThe study covers an evaluation of the influence of extracts (1-50 g/ml), isolated from aerial parts of nine Trifolium L. species (i.e. T. alexandrinum, T. fragiferum, T. hybridum, T. incarnatum, T. pallidum, T. pratense, T. resupinatum var. majus, T. resupinatum var. resupinatum and T. scabrum) on haemostatic properties of blood plasma. MethodsThe clot formation and fibrinolysis assay (CFF), blood clotting times, the extrinsic and intrinsic coagulation pathway-dependent polymerization of plasma fibrin were measured. The effects of plant extracts on amidolytic activity of thrombin were also evaluated and compared with argatroban, an antithrombotic drug. Cytotoxicity was assessed in a model of blood platelets and as the viability of peripheral blood mononuclear cells. Key findingsWhile no changes in blood clotting times or fibrinolytic properties of blood plasma were found, some fractions impaired the blood plasma coagulation induced by the intrinsic coagulation pathway. Reduction in the maximal velocity of fibrin polymerization was also observed in the clot formation and fibrinolysis assay. No cytotoxicity of Trifolium extracts towards the investigated cells was recorded. ConclusionsThe most efficient anticoagulant activity in plasma was found for T. fragiferum and T. incarnatum extracts, while the T. alexandrinum fraction was the most effective inhibitor of thrombin amidolytic activity.
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