Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Lauren M. Bishop, Tong Shen, Oliver Fiehn
Summary: Accurate and reproducible quantification of endogenous lipids is crucial for large-scale lipidomic analyses. Many lipids in liquid chromatography-electrospray ionization-mass spectrometry form various adducts with buffer components, and the mechanisms and conditions affecting adduct formation are poorly understood. Variations in adduct ratios can lead to significant inaccuracies during quantification, and the use of multiple adduct ions improves accuracy compared to using a single adduct ion.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Alexandros Mavrommatis, Maria-Eleftheria Zografaki, Sofia Marka, Eleni D. Myrtsi, Elisavet Giamouri, Christos Christodoulou, Epameinondas Evergetis, Vasilios Iliopoulos, Sofia D. Koulocheri, Georgia Moschopoulou, Panagiotis E. Simitzis, Athanasios C. Pappas, Emmanouil Flemetakis, Apostolis Koutinas, Serkos A. Haroutounian, Eleni Tsiplakou
Summary: This study investigated the carotenoid characterization and antimicrobial potential of Citrus reticulata extract and its effects on broiler chickens' performance, meat quality, and immune-oxidative status. The results showed that Citrus reticulata extract had inhibitory properties against bacteria and improved the immune-oxidative status in broiler chickens.
Article
Chemistry, Analytical
Fei-Long Liu, Tian-Tian Ye, Jiang-Hui Ding, Xiao-Ming Yin, Xiao-Ke Yang, Wei-Hua Huang, Bi-Feng Yuan, Yu-Qi Feng
Summary: Researchers synthesized paired reagents of DMPI and d(3)-DMPI, which can efficiently react with phosphate groups on polar phosphorylated metabolites. They found that tagging DMPI onto ribonucleotides significantly increased ionization efficiencies during LC/MS analysis, resulting in enhanced detection sensitivities.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Pierre Guichard, Michel Laurentie, Dominique Hurtaud-Pessel, Eric Verdon
Summary: An LC-MS/MS method was developed for confirmation of nitrofuran metabolites in meat and aquaculture products, including DNSH. The method showed good trueness and precision, with decision limits and detection limits well within the specified criteria. The sample preparation included a washing step to analyze only protein-bound residues.
Article
Chemistry, Analytical
Tobias Schlappack, Matthias Rainer, Nikolaus Weinberger, Gunther K. Bonn
Summary: This study describes the use of modified halloysite nanotubes as a novel solid material for the efficient solid phase extraction of toxic pyrrolizidine alkaloids. The optimized method showed high extraction efficiencies and overall recoveries for a mixture of pyrrolizidine alkaloids using UHPLC-MS/MS analysis. The novel solid phase material exhibited high selectivity and competitiveness compared to a commercial procedure.
ANALYTICAL METHODS
(2022)
Article
Biochemical Research Methods
Jingfu Zhao, Wenjing Peng, Xue Dong, Yehia Mechref
Summary: Protein-based therapeutics like mAbs play a crucial role in modern medicine, with glycosylation being a key quality attribute affecting the safety and efficacy of biotherapeutics. An LC-MS/MS-based glycoproteomics approach was used to characterize Fc glycans of two NISTmAb samples, revealing differences in galactosylation levels between the modified and unmodified NISTmAb. Additionally, high abundance glycan structures were identified along with relatively low levels of certain glycans and unglycosylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Environmental Sciences
Garth Campbell, Andrea Mannetje, Samuel Keer, Geoff Eagleshama, Xianyu Wang, Chun-Yin Lin, Peter Hobson, Leisa-Maree Toms, Jeroen Douwes, Kevin V. Thomas, Jochen F. Mueller, Sarit Kaserzon
Summary: This study analyzed urine samples from the Australia and New Zealand region and obtained data on the concentrations of glyphosate and its degradation product aminomethylphosphonic acid (AMPA). The results showed that glyphosate was detected in 8% of the urine samples from the Australian general population and in 96% of urine samples from New Zealand farmers. This indicates a widespread presence of glyphosate in the population, with higher detections among farmers.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Margaret L. Eng, Chunyan Hao, Christena Watts, Fengrong Sun, Christy A. Morrissey
Summary: The study utilized sensitive analytical methods to measure the effects of two field-realistic concentrations of neonicotinoid insecticides on red-winged blackbirds. Imidacloprid was rapidly absorbed and metabolized within 48 hours, with the most significant decrease in parent IMI observed at 6 hours post-exposure. 5-OH-IMI was identified as a persistent marker of exposure at 48 hours.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Yumi Komori, Tomoya Niinae, Koshi Imami, Jun Yanagibayashi, Kenichi Yasunaga, Shinya Imamura, Masami Tomita, Yasushi Ishihama
Summary: We developed a bioinertized nanoflow LC/MS/MS system for sensitive analysis of phosphopeptides. Metal ion depletion from the mobile phase and prevention of indirect contact with metal components significantly improved the recovery of phosphopeptides. By inserting an online metal ion removal device, the identified phosphopeptides showed an average increase in peak areas of 9.9-fold overall and 77-fold for multiply phosphorylated peptides.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemical Research Methods
Marketa Simkova, Lucie Kolatorova, Pavel Drasar, Jana Vitku
Summary: A robust and rapid LC-MS/MS method for the quantification of 32 steroid hormones in human plasma was developed. The method was validated for the analysis of C18-, C19-, and C21- steroids, demonstrating its applicability in physiological and pathophysiological conditions. This method will serve as a useful tool for investigating steroid-related diseases and can be expanded to analyze other biological matrices.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Caroline Le Goff, Pierre Lukas, Gael Cobraiville, Marianne Fillet, Anne-Catherine Servais, Pierre Delanaye, Etienne Cavalier
Summary: A LC-MS/MS higher-order method for parathyroid hormone (PTH) analysis was developed, which is independent of antibodies and suitable for a wide range of PTH concentrations. The analytical performance specifications of the method were validated through experiments.
CLINICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Dorothea F. K. Rawn, Guy Dufresne, Genevieve Clement, William D. Fraser, Tye E. Arbuckle
Summary: The MIREC study in Canada found that perfluorinated alkyl substances (PFAS) are commonly present in breast milk, and their concentrations are inversely correlated with age. Primiparous women had higher PFAS concentrations in milk, while the region of maternal birth did not affect PFAS concentrations. Additionally, PFAS with long carbon chains did not exceed detection limits in Canadian breast milk samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemical Research Methods
G. Mayne, E. De Bloois, D. Dabelea, U. Christians
Summary: Neuroactive steroids play a critical role in maternal and fetal health during pregnancy, with implications for perinatal depression and poor pregnancy outcomes. A simple, reliable, and sensitive method for quantifying these compounds is necessary to advance clinical diagnostic tools.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
James B. McAlpine, Shao-Nong Chen, Andrei Kutateladze, John B. MacMillan, Giovanni Appendino, Andersson Barison, Mehdi A. Beniddir, Maique W. Biavatti, Stefan Bluml, Asmaa Boufridi, Mark S. Butler, Robert J. Capon, Young H. Choi, David Coppage, Phillip Crews, Michael T. Crimmins, Marie Csete, Pradeep Dewapriya, Joseph M. Egan, Mary J. Garson, Gregory Genta-Jouve, William H. Gerwick, Harald Gross, Mary Kay Harper, Precilia Hermanto, James M. Hook, Luke Hunter, Damien Jeannerat, Nai-Yun Ji, Tyler A. Johnson, David G. I. Kingston, Hiroyuki Koshino, Hsiau-Wei Lee, Guy Lewin, Jie Li, Roger G. Linington, Miaomiao Liu, Kerry L. McPhail, Tadeusz F. Molinski, Bradley S. Moore, Joo-Won Nam, Ram P. Neupane, Matthias Niemitz, Jean-Marc Nuzillard, Nicholas H. Oberlies, Fernanda M. M. Ocampos, Guohui Pan, Ronald J. Quinn, D. Sai Reddy, Jean-Hugues Renault, Jose Rivera-Chavez, Wolfgang Robien, Carla M. Saunders, Thomas J. Schmidt, Christoph Seger, Ben Shen, Christoph Steinbeck, Hermann Stuppner, Sonja Sturm, Orazio Taglialatela-Scafati, Dean J. Tantillo, Robert Verpoorte, Bin-Gui Wang, Craig M. Williams, Philip G. Williams, Julien Wist, Jian-Min Yue, Chen Zhang, Zhengren Xu, Charlotte Simmler, David C. Lankin, Jonathan Bisson, Guido F. Pauli
NATURAL PRODUCT REPORTS
(2019)
Article
Chemistry, Analytical
Nora Gibitz-Eisath, Miriam Eichberger, Regina Gruber, Sonja Sturm, Hermann Stuppner
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2018)
Article
Chemistry, Medicinal
Mariana Spetea, Silvia B. Rief, Tanila Ben Haddou, Monika Fink, Elka Kristeva, Harald Mittendorfer, Stefanie Haas, Nora Hummer, Valeria Follia, Elena Guerrieri, Muhammad Faheem Asim, Sonja Sturm, Helmut Schmidhammer
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Plant Sciences
Fabian Mayr, Sonja Sturm, Markus Ganzera, Birgit Waltenberger, Stefan Martens, Stefan Schwaiger, Daniela Schuster, Hermann Stuppner
JOURNAL OF NATURAL PRODUCTS
(2019)
Article
Chemistry, Medicinal
Lisa Vieider, Erik Romp, Veronika Temml, Jana Fischer, Christian Kretzer, Martin Schoenthaler, Abdulla Taha, Victor Hernandez-Olmos, Sonja Sturm, Daniela Schuster, Oliver Werz, Ulrike Garscha, Barbara Matuszczak
ACS MEDICINAL CHEMISTRY LETTERS
(2019)
Article
Medical Laboratory Technology
Simon Moosmang, Maria Pitscheider, Sonja Sturm, Christoph Seger, Herbert Tilg, Maria Halabalaki, Hermann Stuppner
CLINICA CHIMICA ACTA
(2019)
Article
Food Science & Technology
Simon Moosmang, Aino Siltari, Marie-Theres Bolzer, Stefan Kiechl, Sonja Sturm, Hermann Stuppner
INTERNATIONAL DAIRY JOURNAL
(2019)
Article
Chemistry, Analytical
Nora Gibitz-Eisath, Miriam Eichberger, Regina Gruber, Christoph Seger, Sonja Sturm, Hermann Stuppner
JOURNAL OF SEPARATION SCIENCE
(2020)
Article
Nutrition & Dietetics
Simon J. Reider, Simon Moosmang, Judith Tragust, Lovro Trgovec-Greif, Simon Tragust, Lorenz Perschy, Nicole Przysiecki, Sonja Sturm, Herbert Tilg, Hermann Stuppner, Thomas Rattei, Alexander R. Moschen
Article
Plant Sciences
Simon Moosmang, Sonja Sturm, Johannes Novak, Brigitte Lukas, Hermann Stuppner
Article
Agriculture, Multidisciplinary
Verena Gratl, Sonja Sturm, Elena Zini, Thomas Letschka, Marco Stefanini, Silvia Vezzulli, Hermann Stuppner
Summary: The study comparing the polyphenolic profiles of hybrid grapevine varieties from international breeding programs and private initiatives in the same vineyard showed similar overall profiles but some variations in specific compounds. This research will help in selecting promising candidates for further breeding programs aimed at developing highly resistant grape varieties with improved sensory characteristics.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Multidisciplinary Sciences
Teresa Kaserer, Theresa Steinacher, Roman Kainhofer, Filippo Erli, Sonja Sturm, Birgit Waltenberger, Daniela Schuster, Mariana Spetea
SCIENTIFIC REPORTS
(2020)
Article
Plant Sciences
Sophia Mayr, Simon Strasser, Christian G. Kirchler, Florian Meischl, Stefan Stuppner, Krzysztof B. Bec, Justyna Grabska, Sonja Sturm, Hermann Stuppner, Michael A. Popp, Gunther K. Bonn, Christian W. Huck
Summary: Near-infrared spectroscopy was used to analyze the content of silymarin and individual flavonolignans in milk thistle seeds. The benchtop device NIRFlex N-500 showed the best performance, while quantum chemical simulation of spectra helped to understand the impact of chemical and physical properties on analysis. Miniaturized near-infrared spectroscopy proved to be capable of rapid and nondestructive quantification of silymarin and flavonolignans in whole milk thistle seeds.
Article
Biochemistry & Molecular Biology
Sonja Sturm, Christina Hoegner, Christoph Seger, Hermann Stuppner
Summary: The study investigated the in vitro metabolism of vitetrifolin D using HPLC-DAD-QTOF-MS and HPLC-SPE-NMR, identifying 27 phase I and phase II metabolites, with structural characterization of nine phase I metabolites. By avoiding preparative isolation of metabolites, the study demonstrated the successful performance of in vitro metabolism studies using hyphenated instrumental analysis methodologies even with limited substance availability.
Review
Cell Biology
Christoph Seger, Sonja Sturm
Summary: NMR spectroscopy is essential for determining the structural information of analytes from natural sources in liquid state. While mass spectrometry can differentiate and group biomarkers, the structural characterization often requires the use of NMR spectroscopy.
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)