期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 156, 期 -, 页码 284-296出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2018.04.044
关键词
TCM Qianjinteng; Stephania species; Quality control; Effective alkaloids; LC-QTOF-MS; OPLS-DA
资金
- National Natural Science Foundation of China [81673323, 81473108]
- Open Project Fund of Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education [NRFM201703]
- Long-term Training of Young Teachers in Shenyang Pharmaceutical University [ZQN2015007]
A method for rapid identification of bioactive components in herbals from Stephania species was developed by utilizing UPLC-QTOF-MS/MS in this study. Sixty-three alkaloids including the types of proaporphine, aporphine, protoberberine, morphine, hasubanan, benzylisoquinoline and bisbenzylisoquinoline were characterized. Among them, 29 components were selected for semi-quantitative analyses to characterize the difference of chemical compositions among three Stephania species. The results revealed that bisbenzylisoquinoline-type of alkaloids are the representative components of SB (Subgen. Botryodiscia). The content of aporphine-type of alkaloids was much higher in ST (Subgen. Tuberiphania) than that in SS (Subgen. Stephania), while the content of protoberberine-type of alkaloids was higher than that in ST. The present study offered an efficient approach for quality control of Qianjinteng based on the rapid identification and semi-quantification of alkaloids in Stephania species by using UPLC-QTOF-MS. (C) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据