Article
Biochemical Research Methods
Priscila D. Mendonca, Lorenna K. B. Santos, Marcos Foguel, Marco A. B. Rodrigues, Marli T. Cordeiro, Luis M. Goncalves, Ernesto T. A. Marques, Rosa F. Dutra
Summary: The incidence of dengue virus infection has grown dramatically worldwide, affecting 128 countries. The diagnosis is challenging due to similarities with other viruses such as Zika, and the underreporting of cases leads to potentially higher numbers of affected people. An immunosensor using carbon nanotube-ethylenediamine for NS1 detection shows promising results for diagnosis of dengue and Zika viruses.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Linh Tran, Sangkwon Park
Summary: A highly sensitive immunosensor using quantum dots and streptavidin was developed for detecting the dengue biomarker NS1, offering a fast, convenient, and sensitive approach. However, interference in human plasma serum solution was observed, suggesting the need for further solutions.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Brandon E. K. Tan, Michael R. Beard, Nicholas S. Eyre
Summary: In this study, random point mutagenesis was used to identify residues within NS1 that are essential for its secretion. Ten point mutations were identified that corresponded with impaired NS1 secretion. Additional studies on two of these mutants, V220D and A248V, revealed that they prevented viral RNA replication and disrupted NS1 processing or maturation.
Article
Infectious Diseases
Tanapan Prommool, Pongpawan Sethanant, Narodom Phaenthaisong, Nattaya Tangthawornchaikul, Adisak Songjaeng, Panisadee Avirutnan, Dumrong Mairiang, Prasit Luangaram, Chatchawan Srisawat, Watchara Kasinrerk, Sirijitt Vasanawathana, Kanokwan Sriruksa, Wannee Limpitikul, Prida Malasit, Chunya Puttikhunt
Summary: The study modified the stNS1-ELISA method by introducing biotinylated serotype-specific antibodies and a new DENV2-specific antibody, resulting in a significant improvement in sensitivity for dengue virus serotype detection. The modified assay is efficient for early dengue diagnosis and epidemiological studies.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Infectious Diseases
Samuel Santos Pereira, Robert Andreata-Santos, Lennon Ramos Pereira, Camila Pereira Soares, Alvina Clara Felix, Patricia de Mello Jungmann Cardoso de Andrade, Edison Luis Durigon, Camila Malta Romano, Luis Carlos de Souza Ferreira
Summary: The study aimed to achieve greater specificity in DENV serological tests by using a recombinant antigen (Delta NS1) to differentiate DENV and ZIKV. The Delta NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive samples, with a sensitivity of 82% and specificity of 93%. This method may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Thanyaporn Dechtawewat, Sittiruk Roytrakul, Yodying Yingchutrakul, Sawanya Charoenlappanit, Bunpote Siridechadilok, Thawornchai Limjindaporn, Arunothai Mangkang, Tanapan Prommool, Chunya Puttikhunt, Pucharee Songprakhon, Kessiri Kongmanas, Nuttapong Kaewjew, Panisadee Avirutnan, Pa-thai Yenchitsomanus, Prida Malasit, Sansanee Noisakran
Summary: This study uncovered the phosphorylation modification of the dengue virus NS1 protein, with mutational analysis showing specific phosphorylation sites affecting DENV infectivity and the production of NS1 dimers post-infection.
Article
Chemistry, Physical
Zixuan Zhang, Wei Li, Jie Liu, Ying Li, Jingfu Qiu, Chaorui Li
Summary: This research proposes an electrochemical immunosensor based on the Al-TCPP adsorption aggregation signal amplification approach for the high sensitivity detection of the dengue virus NS1 protein. The immunosensor utilizes a metal-organic framework material and methylene blue to amplify the electrical signal. It exhibits good reproducibility and stability, with a wide detection range and low detection limit.
Article
Virology
Ana C. Alcala, Jose L. Maravillas, David Meza, Octavio T. Ramirez, Juan E. Ludert, Laura A. Palomares
Summary: Dengue is a common viral disease transmitted by mosquitoes. This study reveals that the dengue virus NS1 enters cells through the human HDL receptor, which has significant implications for viral replication and pathogenesis.
JOURNAL OF VIROLOGY
(2022)
Article
Biophysics
Shashank K. Gahlaut, Anisha Pathak, Banshi D. Gupta, J. P. Singh
Summary: The platform utilizes specific antibodies for early detection of Dengue virus NS1 antigen, providing high sensitivity and detection limit. The technology shows potential for very early detection in infected blood samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Elakkiya Elumalai, M. Suresh Kumar
Summary: In this study, the NS1 protein of Dengue virus was modeled and simulated, and the compound neo-andrographolide was found to have the potential to inhibit the activity of NS1, thereby inhibiting Dengue virus replication.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Immunology
Nikole L. L. Warner, Susan B. B. Core, Kathryn M. M. Frietze
Summary: Dengue virus is a global health problem and there is a need for more effective vaccines. This study identified peptide epitopes of the non-structural protein 1 of the virus that could induce antibodies capable of binding to infected cells. These peptides may be potential targets for future vaccine development.
Article
Virology
Gerson I. Caraballo, Romel Rosales, Mercedes Viettri, Juan Manuel Castillo, Raymundo Cruz, Siyuan Ding, Harry B. Greenberg, Juan E. Ludert
Summary: Dengue is the most important mosquito-borne viral disease to humans. Dengue virus NS1 is a multifunctional protein essential for replication and modulation of innate immunity. The protein interactome of DENV NS1 in mosquito cells was investigated, revealing interactions with various mosquito protein targets. This study provides novel insights into the functions of NS1 and its role in promoting viral replication, as well as identifying new protein pathways involved in mosquito cells.
JOURNAL OF VIROLOGY
(2022)
Article
Chemistry, Analytical
Kanaporn Poltep, Emi E. Nakayama, Tadahiro Sasaki, Takeshi Kurosu, Yoshiki Takashima, Juthamas Phadungsombat, Nathamon Kosoltanapiwat, Borimas Hanboonkunupakarn, Sarin Suwanpakdee, Hisham A. Imad, Narinee Srimark, Chiaki Kitamura, Atsushi Yamanaka, Akio Okubo, Tatsuo Shioda, Pornsawan Leaungwutiwong
Summary: The research team successfully developed serotype-specific immunochromatography systems for all four DENV serotypes, showing high specificity and sensitivity. These systems can rapidly detect DENV with good performance in laboratory and clinical validation. In DENV-endemic areas, these assay systems could accelerate both infection diagnosis and epidemiologic studies.
Article
Virology
Jitu M. Kalita, Alisha Aggarwal, Kavita Yedale, Ravisekhar Gadepalli, Vijaya L. Nag
Summary: This study in western India revealed a higher seroprevalence of dengue infection and a gradual increase in seroprevalence in the region. Most laboratory-confirmed dengue cases were observed in the 21-30 age group. The most common serotype detected in the tested samples was DEN-3.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Paula X. Losada, Irene Bosch, Galit H. Frydman, Lee Gehrke, Carlos F. Narvaez
Summary: This study investigated the effects of DENV-2 and ZIKV infection on human platelet precursors, revealing that DENV-2 induced higher levels of cell death, gene expression, and reduced release of PLPs compared to ZIKV infection.
Article
Infectious Diseases
Kah Wai Looi, Yukari Matsui, Mari Kono, Chandramathi Samudi, Nozomi Kojima, Jin Xu Ong, Chin Aun Tan, Chong Siang Ang, Peter Hao Yuan Tan, Hemalatha Shamnugam, Shamala Devi Sekaran, Sharifah Faridah Syed Omar, Lucy Chai See Lum
Summary: This study evaluated the trend of Immature Platelet Fraction (IPF) as an early recovery indicator of platelets in dengue patients with thrombocytopenia, and its relationship with severe dengue in conjunction with reticulocyte count. The results showed that IPF% increased significantly in severe dengue patients on days 3-5 after the onset of fever, and reticulocyte count also increased significantly on day 5. IPF can be utilized as an early recovery indicator of platelets in patients with dengue and thrombocytopenia.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemical Research Methods
Ying Yao Chia, T. Malathi Theverajah, Yatimah Alias, Sook Mei Khor
Summary: A novel method of multiplex analysis was proposed using three-dimensional porous calcium alginate beads for detecting serum-HER2 and serum-CA125 breast cancer biomarkers, with high sensitivity and improved linear dynamic range. The developed system showed good agreement with standard methods when analyzing real clinical samples, and the analysis only required approximately 30 minutes. This approach has the potential for use in multiplex assays and biosensors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Food Science & Technology
Pei Chi Ng, Nur Amy Syahira Ahmad Ruslan, Ling Xuan Chin, Musa Ahmad, Sharina Abu Hanifah, Zanariah Abdullah, Sook Mei Khor
Summary: The increasing public awareness of food quality and safety has led to a rapid growth in halal food authentication. Analytical methods are being researched extensively to ensure accurate and reliable results for monitoring and controlling the authenticity of halal food. The review provides an impartial overview of recent studies on analytical techniques for halal food authenticity, highlighting the need for advancements in technology and methodologies.
JOURNAL OF FOOD SCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Sook Mei Khor, Joonhwa Choi, Phillip Won, Seung Hwan Ko
Summary: This review discusses the challenges and limitations of wearable biosensors for continuous sweat monitoring, particularly in the management of type II diabetes. It also proposes ten basic criteria for the development of a suitable, workable, and wearable sweat-based glucose biosensor. The insights provided in this review are important for the effective management and health monitoring of type II diabetes.
Review
Chemistry, Analytical
Lai Mun Koh, Sook Mei Khor
Summary: This paper compares and contrasts sensors capable of identifying biodegradable polymers and discusses their advantages and limitations. The use of sensors for biodegradability detection represents an innovative approach, and there is a wide range of sensor materials with different types and applications.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Chang Hong Voon, Nurida Mohd Yusop, Sook Mei Khor
Summary: This article reviews the advantages, challenges, and limitations of using a whole-cell bioluminescent biosensor technology to measure oil and grease content in wastewater. It attempts to bridge the knowledge gap between conventional laboratory methods and biosensor technology, providing guidance for real-world applications.
Article
Immunology
Felicita Fedelis Jusof, Chai K. Lim, Fazidatul Nadhirah Aziz, Hui Jen Soe, Chandramathi Samudi Raju, Shamala Devi Sekaran, Gilles J. Guillemin
Summary: This study found that the release of cytokines by immune cells increases as the severity of dengue infection worsens, leading to activation of the kynurenine pathway (KP). Specific biomarkers and cytokines (CXCL10, CCL2, and AA) can predict the risk of developing dengue with warning signs (DWS+). These findings suggest a potential role for these markers in the early detection and management of dengue.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Pharmacology & Pharmacy
Hui Ling Ong, Choo Hock Tan, Louisa Pernee Lee, Sook Mei Khor, Kae Yi Tan
Summary: This study developed an immunodetection assay for the diagnosis of cobra envenoming. The assay can accurately differentiate cobra venoms from other snake species and has high sensitivity for detecting cobra venoms.
Review
Immunology
Shamala Devi Sekaran, Amni Adilah Ismail, Gaythri Thergarajan, Samudi Chandramathi, S. K. Hanan Rahman, Ravishankar Ram Mani, Felicita Fedelis Jusof, Yvonne A. L. Lim, Rishya Manikam
Summary: Dengue and Zika viruses are major public health concerns, affecting millions of people worldwide. The similarities and differences in disease transmission and immune responses between these two viruses are crucial for understanding and tackling these infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sharifah Aliah Diyanah Syed Hussin, Ang-Lim Chua, Hassanain Al-Talib, Shamala Devi Sekaran, Seok Mui Wang
Summary: This review paper summarizes the main laboratory diagnostic methods for central nervous system infections caused by viruses, ranging from conventional isolation methods to molecular detections. Molecular detections have many advantages, such as high sensitivity, rapidity, and both qualitative and quantitative results.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2022)
Review
Chemistry, Analytical
Jia Ling Tsong, Rodney Robert, Sook Mei Khor
Summary: Glove-based wearable chemical sensors provide surface analysis for various samples by swiping glove sensors, and they are widely used in crime scene investigation, airport security, and disease control. They overcome the limitations of portable sensors and outperform other wearable sensors in healthcare monitoring. This article discusses the challenges and criteria for selecting glove materials and nanomaterials, introduces transducer modification techniques, and evaluates the Sustainable Development Goals and disposal strategies. The provided tables offer a quick comparison of the features and functionalities of different glove-based wearable sensors.
ANALYTICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
J. Wu, Q. Wang, W. Wu, S. M. Khor
Summary: This research aims to investigate a better material for water purification and successfully synthesized absorbent cotton-templated manganese oxide nanocomposites (AC/MnO2). The AC/MnO2 showed excellent adsorption capacity for dye wastewater degradation in aqueous solutions, and the optimal conditions for adsorption were determined. The results also demonstrated that the AC/MnO2 can be regenerated and reused.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Computer Science, Interdisciplinary Applications
Aizatul Shafiqah Mohd Faizal, Wei Yin Hon, T. Malathi Thevarajah, Sook Mei Khor, Siow-Wee Chang
Summary: A machine learning approach was used to identify potential biomarkers for early detection and treatment of acute myocardial infarction (AMI). It was found that using fewer features generally performed better than using all features, and a five-feature model including cardiac troponin I, HDL cholesterol, HbA1c, anion gap, and albumin was identified as potential biomarkers to predict the prognosis of AMI patients.
MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
(2023)
Article
Pathology
Gaythri Thergarajan, Shamala Devi Sekaran
Summary: Every year, the incidence of dengue fever increases significantly and accounts for 10% of fever cases in children and adolescents in endemic countries. The diagnosis of dengue has been challenging due to its similarity to symptoms of other viruses, and the lack of sensitive diagnostic tools may contribute to the rise in dengue incidence.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Review
Chemistry, Analytical
Jia Ling Tsong, Sook Mei Khor
Summary: Smart and precision farming utilizes advanced technology such as sensors, AI, and IoT to improve decision-making and increase agricultural productivity in the face of unpredictable natural disasters, disease outbreaks, climate change, pollution, and war. Biosensors, as a rising technology, have the potential to promote smart and precision farming in developing and underdeveloped countries.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)