The effect of catalase C262T gene polymorphism in susceptibility to ovarian cancer in Kermanshah province, Western Iran

Ovarian cancer has a heterogeneous biology and behaviour. Oxidative stress can initiate chronic inflammation, which can sequentially facilitate chronic diseases, including cancer. Oxidative stress may arise when there is extra reactive oxygen species (ROS) production and/or inadequate defence mechanisms. There are some antioxidant defences that can fight against oxidative damage, including catalase (CAT) enzyme. We sought to evaluate the association of CAT C262T gene polymorphism with increased risk of ovarian cancer. A total of 74 paraffin-embedded ovarian cancer blocks were taken from the archive of Imam-Reza Hospital, Kermanshah University of Medical Sciences, between 2010 and 2014. Also, 153 blood samples were harvested from healthy volunteers. For genotyping of CAT C262T, we designed allele-specific polymerase chain reaction (AS-PCR). T' allele of CAT C262T showed a protective effect against the risk of ovarian cancer [OR=0.4 (95% CI 0.25-0.6), p value <.001]. Calculating adjusted odds ratio showed the distribution of alleles and genotypes was not affected by age. The present study reported a significant association between the distribution of CAT C262T gene polymorphism and ovarian cancer for the first time in a sample of the Iranian population.Impact StatementWhat is already known on this subject: Ovarian cancer has a heterogeneous biology and behaviour at the clinical, cellular and molecular aspects. Ovulation releases follicular fluid containing reactive oxygen species which is related to changes in the microenvironment, such as inflammation, that could be a factor in early ovarian carcinogenesis. There are some antioxidant defences that can protect cells against oxidative damage, including catalase (CAT). Different studies investigated the relationships between CAT C262T polymorphism and several diseases. Belotte etal. (2015), for the first time, indicated no significant association between CAT C262T and the risk of ovarian cancer, while they showed this SNP is associated with poor survival and therefore may serve as a prognostic factor for ovarian cancer.What the results of this study add: In the present study, T' allele of CAT C262T showed a protective effect against the risk of ovarian cancer. Calculating adjusted odds ratio showed that the distribution of alleles and genotypes is not affected by age.What the implications are of these findings for clinical practice and/or further research: Pair-wise genetic analyses using SNPSTATS software showed that this genotyping was more compatible with recessive models, i.e. two copies of the associated variant are required to increase the risk of ovarian cancer. Further research about other antioxidant genes in a larger population is needed to predict the risk of ovarian cancer and survival rate of patients.