期刊
JOURNAL OF NEUROSCIENCE
卷 38, 期 20, 页码 4774-4790出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0360-18.2018
关键词
cerebellins; neurexins; synapse formation; synapse specification; synaptic organizers
资金
- National Institutes of Health-National Institute of Mental Health [R37-MH052804]
Cerebellins are synaptic organizer molecules that bind to presynaptic neurexins and postsynaptic receptors. They are well studied in the cerebellum, but three of the four cerebellins (Cblnl, Cbln2, and Cbln4) are also broadly expressed outside of the cerebellum, suggesting that they perform general functions throughout the brain. Here, we generated male and female constitutive single (KO), double KO (dKO), and triple KO (tKO) mice of Cblnl, Cbln2, and Cbln4. We found that all constitutive cerebellin-deficient mice were viable and fertile, suggesting that cerebellins are not essential for survival. Cblnl/2 dKO mice exhibited salience-induced seizures that were aggravated in Cbln 1/2/4 tKO mice, suggesting that all cerebellins contribute to brain function. As described previously, Cblnl KO mice displayed major motor impairments that were aggravated by additional KO of Cbln2. Strikingly, the Cblnl/2 dKO did not cause alterations in synapse density in the hippocampus of young adult (1 - and 2-month-old) mice, but produced a selective -50% decrease in hippocampal synapse density in the stratum lacunosum moleculare of the CA1 region and in the dentate gyrus of aging, 6-month-old mice. A similar decrease in excitatory synapse density was observed in the striatum and retrosplenial cortex. Behaviorally, the Cblnl KO produced dramatic changes in motor behaviors that were partly aggravated by additional deletion of Cbln2 and/or Cbln4. Our results show that cerebellins are not essential for survival and do not contribute to initial synapse formation, but perform multiple functions throughout the brain; as a consequence, their ablation results in a delayed loss of synapses and in behavioral impairments.
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