Article
Pharmacology & Pharmacy
Lena Radtke, Aleksandra Majchrzak-Celinska, Charles Awortwe, Inga Vater, Inga Nagel, Susanne Sebens, Ingolf Cascorbi, Meike Kaehler
Summary: This study demonstrates the significance of ABCB1 in GBM cells, affecting the response to temozolomide and carmustine treatment, while ABCG2 plays a marginal role.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Janaina Alessandra Silva, Alison Colquhoun
Summary: This study investigated the effects of polyunsaturated fatty acids (PUFAs) on U87MG cells and TMZ-resistant cells. PUFAs altered the fatty acid composition and caused lipid droplet accumulation in the cytoplasm. EPA had the most significant inhibitory effect on cell growth and GLA and EPA had larger effects on ABC transporter expression than DHA. Overall, PUFAs can modulate ABC transporters in glioblastoma cells.
Article
Biochemistry & Molecular Biology
Jianyi Li, Yuntong Lv, Kunpeng Yan, Fengting Yang, Xuewei Chen, Xiwu Gao, Shuyuan Wen, Hongfei Xu, Yiou Pan, Qingli Shang
Summary: The study revealed that ATP-binding cassette transporters (ABCs) play important roles in cyantraniliprole resistance. Inhibition of ABCs increased the toxicity of cyantraniliprole, while overexpression of ABCs in fruit flies enhanced their tolerance to cyantraniliprole. Suppression of specific ABC transporters increased the sensitivity of cyantraniliprole-resistant strains. These findings provide valuable insights for resistance management strategies.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Oncology
Julia A. Schulz, Louis T. Rodgers, Richard J. Kryscio, Anika M. S. Hartz, Bjorn Bauer
Summary: This study characterized two human GBM models (U87-luc2, U251-RedFLuc) and found that they recapitulate different aspects of GBM disease, with U251-RedFLuc cells showing higher invasiveness. The findings highlight the importance of considering GBM heterogeneity in preclinical research.
Article
Microbiology
Magali Van den Kerkhof, Philippe Leprohon, Dorien Mabille, Sarah Hendrickx, Lindsay B. Tulloch, Richard J. Wall, Susan Wyllie, Eric Chatelain, Charles E. Mowbray, Stephanie Braillard, Marc Ouellette, Louis Maes, Guy Caljon
Summary: Oxaboroles, a novel class of antileishmanial drugs, have not shown readily emergent resistance and hold promise for future treatment. The combination of a genome-wide cosmid library and next-generation sequencing can identify resistance determinants and potential targets.
Article
Agriculture, Multidisciplinary
Yuntong Lv, Kunpeng Yan, Xiwu Gao, Xuewei Chen, Jianyi Li, Yaping Ding, Hang Zhang, Yiou Pan, Qingli Shang
Summary: This study explores the involvement of ATP-binding cassette (ABC) transporters in spirotetramat resistance in cotton aphids. The results show that several ABC transporter genes, including ABCB4, ABCB5, ABCF2, MRP11, and MRP12, are upregulated in the spirotetramat-resistant strains. Functional analysis confirms the contribution of these genes to spirotetramat tolerance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yanyan Ma, Zhanchen Guo, Chuanwen Fan, Jingran Chen, Shuxin Xu, Zhen Liu
Summary: This study developed aptamers that can target ABCG2 and reverse multidrug resistance, showing great potential in cancer diagnosis and treatment. The aptamers can specifically bind to human colorectal cancer stem cells and effectively reverse drug resistance in liver cancer cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Jan Viglas, Petra Olejnikova
Summary: The ABC transporter superfamily plays a crucial role in transporting various molecules across biological membranes, including antifungal compounds in filamentous fungi. Understanding the physiological substrates and roles of individual subfamilies of ABC transporters in fungi is important for clarifying their significance in clinical and agricultural settings.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yuntong Lv, Yiou Pan, Jianyi Li, Yaping Ding, Zihan Yu, Kunpeng Yan, Qingli Shang
Summary: Clarifying the molecular mechanisms of cotton aphid resistance to various insecticides is crucial for safe insecticide application. This study reveals that ABCB5, ABCF2, and MRP12 are negatively regulated by the transcription factor CF2-II, leading to increased resistance to certain insecticides. These findings contribute to a better understanding of the adaptation mechanism of ABC transporters in the context of multi-insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Georgios Sofianos, Anastasios Samaras, Georgios Karaoglanidis
Summary: Botrytis cinerea is a high-risk pathogen for fungicide resistance development. The study found that MLR/MDR strains are present in Greek populations of strawberries, rootstocks, and tomatoes, with notably high frequencies of resistance. The combination of MLR and MDR mutations leads to even higher levels of fungicide resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Danijela D. Nikodijevic, Jovana V. Jovankic, Danijela M. Cvetkovic, Marko Z. Andelkovic, Aleksandra G. Nikezic, Milena G. Milutinovic
Summary: The study evaluated the potential antitumor activity of snake venom and L-amino acid oxidase on colorectal cancer cells, showing that snake venom primarily led to necrosis while L-amino acid oxidase induced apoptosis correlated with oxidative imbalances in cancer cells. Additionally, the gene expression profiles and levels of membrane transporters also impacted the sensitivity of treatment across different cell lines. The findings suggest that L-amino acid oxidase could be a promising substance for further research on its antitumor effects, potentially with lower risk of drug resistance in vitro.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Agronomy
Jinqiong Shan, Xi Sun, Ran Li, Bin Zhu, Pei Liang, Xiwu Gao
Summary: Overexpression of certain genes in the ABCG subfamily is implicated in the resistance of Plutella xylostella to chlorantraniliprole. These findings could provide a foundation for further exploration of the role of ABC transporters in chlorantraniliprole resistance in Plutella xylostella and other insect pests.
PEST MANAGEMENT SCIENCE
(2021)
Review
Pharmacology & Pharmacy
Ragini Amarnani, Amey Revdekar, Bhagyashree Salvi, Pravin Shende
Summary: Some existing therapies are not effective in treating drug-resistant infections due to low drug efficacy, increasing dosage regimes, bacteria mutations, and poor drug properties. Overuse of antibiotics leads to the emergence and spread of resistant microorganisms. Nanocarriers with ABC transporter efflux mechanism can overcome multidrug resistance by interfering with normal cell activity. This review focuses on the novel applications of nanocarriers using the ABC transporter pump to overcome resistance in various organs.
DRUG DISCOVERY TODAY
(2023)
Review
Pharmacology & Pharmacy
Lenka Hruba, Viswanath Das, Marian Hajduch, Petr Dzubak
Summary: Nucleoside-based drugs, widely used in the treatment of various diseases, have been hindered by drug resistance. This review explores the mechanisms of resistance, including changes in membrane transporters, activating kinases, drug elimination strategies, and DNA damage repair mechanisms. The critical analysis in this review points towards novel therapeutic strategies such as targeted therapies, new nucleoside analogs, and synergistic drug combinations to overcome resistance and improve patient outcomes.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dandan Zhang, Minghui Jin, Yanchao Yang, Jianfeng Zhang, Yongbo Yang, Kaiyu Liu, Mario Soberon, Alejandra Bravo, Yutao Xiao, Kongming Wu
Summary: The evolution of resistance in target pests reduces the efficacy of Bacillus thuringiensis Cry toxins in insect-pest control. Mutations in Cadherin (CAD) or ATP-binding cassette (ABC) transporter genes have been linked to Cry resistance in different pests, and there is a synergistic interaction between ABCC2 and CAD in Cry toxicity. Studies on isogenic lines of Helicoverpa armigera showed significantly enhanced Cry1Ac resistance when both HaABCC2 and HaCAD mutations were present, suggesting a synergistic effect between CAD and ABCC2.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Clinical Neurology
Mohammed H. Ahmed, Isaias Hernandez-Verdin, Franck Bielle, Maite Verreault, Julie Lerond, Agusti Alentorn, Marc Sanson, Ahmed Idbaih
Summary: CD80 and CD86 are heterogeneously expressed in the tumor microenvironment of glioblastomas. Elevated mRNA expression of both CD80 and CD86 is associated with shorter progression free survival. CD86 may serve as a potential biomarker for prognosis in GBM patients treated with immunotherapy.
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES
(2023)
Review
Oncology
Johan M. Kros, Elisabeth Rushing, Aime L. Uwimana, Aurelio Hernandez-Lain, Alex Michotte, Maysa Al-Hussaini, Franck Bielle, Christian Mawrin, Gianluca Marucci, C. Mircea S. Tesileanu, Roger Stupp, Brigitta Baumert, Martin van den Bent, Pim J. French, Thierry Gorlia
Summary: The histologic parameters, especially the mitotic count, provide additional prognostic value in IDH mutant astrocytomas. The mitotic count significantly affects the PFS and marginally the OS. Furthermore, the mitotic count also affects the PFS of tumors with CDKN2A/B homozygous deletion.
Article
Clinical Neurology
Julien Boetto, Franck Bielle, Suzanne Tran, Pauline Marijon, Matthieu Peyre, Valerie Rigau, Michel Kalamarides
Summary: This study confirms the reproducibility and prognostic value of the GAB1 marker in anterior skull base meningiomas, suggesting its usefulness for prognostic evaluation and identification of Hedgehog-activated meningiomas.
Article
Clinical Neurology
Matthieu Peyre, Suzanne Tran, Beatrice Parfait, Isabelle Bernat, Franck Bielle, Michel Kalamarides
Summary: Neurofibromatosis type 2 (NF2) is a rare genetic disorder characterized by central nervous system lesions, but it can also lead to peripheral nerve pathology causing pain and sensory loss. This study investigated the tumor burden of peripheral nerve pathology in NF2 patients and found that surgery is a safe and effective method for treating peripheral nerve schwannomas-associated pain in NF2, except for rare multinodular tumors.
Article
Pharmacology & Pharmacy
Mohammed H. H. Ahmed, Isaias Hernandez-Verdin, Emie Quissac, Nolwenn Lemaire, Coralie Guerin, Lea Guyonnet, Noel Zahr, Laura Mouton, Mathieu Santin, Alexandra Petiet, Charlotte Schmitt, Guillaume Bouchoux, Michael Canney, Marc Sanson, Maite Verreault, Alexandre Carpentier, Ahmed Idbaih
Summary: Therapeutic antibodies targeting immune checkpoints have limited efficacy in clinical trials for glioblastoma patients. Ultrasound-mediated blood-brain barrier opening (UMBO) improves drug delivery to the brain. In preclinical models of glioblastoma, UMBO combined with immune checkpoint inhibitors showed increased overall survival and improved drug penetration.
Article
Biotechnology & Applied Microbiology
Pierre-Cyril Comes, Tuan Le Van, Suzanne Tran, Solene Huard, Samiya Abi-Jaoude, Quitterie Venot, Pauline Marijon, Julien Boetto, Antoine Blouin, Franck Bielle, Yohan Ducos, Yu Teranishi, Michel Kalamarides, Matthieu Peyre
Summary: Despite their rarity, PIK3CA mutations in meningiomas have raised interest as potentially targetable, ubiquitous mutations owing to their presence in sporadic benign and malignant tumors but also in hormone-related cases. The researchers used genetically engineered mouse models to demonstrate that Pik3ca mutations in postnatal meningeal cells are sufficient to promote meningioma formation but also tumor progression in mice. They also found that hormone impregnation fails to induce meningioma tumorigenesis while promoting breast tumor formation.
CANCER GENE THERAPY
(2023)
Article
Oncology
Ivan Caramanna, Jaap C. Reijneveld, Peter M. van de Ven, Martin van de Bent, Ahmed Idbaih, Wolfgang Wick, Martin J. B. Taphoorn, Linda Dirven, Andrew Bottomley, Martin Klein
Summary: This study evaluated the cognitive awareness of patients with recurrent high-grade glioma (HGG) and found an association between neurocognitive functioning and complaints. The findings suggest that patients' awareness of their neurocognitive limitations should be considered in clinical decision-making and interpretation of patient-reported outcomes (PROs).
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Roseline Vibert, Jasmine Hasnaoui, Alexandre Perrier, Alexandra Lefebvre, Chrystelle Colas, Marion Dhooge, Noemie Basset, Albain Chansavang, Camille Desseignes, Alex Duval, Solenne Farelly, Nadim Hamzaoui, Pierre Laurent-Puig, Julie Metras, Diane Moliere, Martine Muleris, Jeanne Netter, Mehdi Touat, Franck Bielle, Karim Labreche, Romain Nicolle, Geraldine Perkins, Mathilde Warcoin, Florence Coulet, Patrick R. Benusiglio
Summary: Some patients with Lynch syndrome (LS) exhibit extreme phenotypes, such as cancer at an early age or cancer types without screening guidelines. This study investigated the role of additional germline variants in cancer susceptibility genes (CSG) in explaining these phenotypes. Comparison of LS patients with early-onset (EO) and usual-onset (UO) cancer diagnoses revealed an excess of pathogenic variants and variants of unknown significance in the gastrointestinal CSG for the EO group. Notable germline variants were identified, suggesting the consideration of additional variants in future screening recommendations to better assess cancer risk.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Aravindan Arun Nadaradjane, Julien Diharce, Joseph Rebehmed, Frederic Cadet, Fabrice Gardebien, Jean-Christophe Gelly, Catherine Etchebest, Alexandre G. de Brevern
Summary: Heavy Chain Only Antibodies are specific to Camelid species and their heavy chain variable domain (VHH) holds promise for research and therapy. However, due to limited experimental structures, structural model prediction for VHH is necessary. This study assesses and compares the quality of predictions from various modelling methodologies.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Oncology
Archita Biswas, Manuela Salvucci, Kate Connor, Heiko Dussmann, Steven Carberry, Michael Fichtner, Ellen King, Brona Murphy, Alice C. O'Farrell, Jane Cryan, Alan Beausang, Josephine Heffernan, Mattia Cremona, Bryan T. Hennessy, James Clerkin, Kieron J. Sweeney, Steve MacNally, Francesca Brett, Philip O'Halloran, Orna Bacon, Simon Furney, Maite Verreault, Emie Quissac, Franck Bielle, Mohammed H. Ahmed, Ahmed Idbaih, Sieger Leenstra, Ioannis Ntafoulis, Federica Fabro, Martine Lamfers, Anna Golebiewska, Frank Hertel, Simone P. Niclou, Romain Tching Chi Yen, Andreas Kremer, Gonca Dilcan, Francesca Lodi, Ingrid Arijs, Diether Lambrechts, Manasa Kalya Purushothama, Alexander Kel, Annette T. Byrne, Jochen H. M. Prehn
Summary: This study investigated molecular differences between long-term survivors (LTS) and short-term survivors (STS) of glioblastoma (GBM). Cilium gene signatures were found to be enriched in LTS tumors, while STS tumors exhibited abnormal protein expression. These findings provide potential biomarkers and therapeutic targets for the management of GBM.
JOURNAL OF NEURO-ONCOLOGY
(2023)
Review
Oncology
Nicolas Crainic, Julia Furtner, Johan Pallud, Franck Bielle, Giuseppe Lombardi, Roberta Ruda, Ahmed Idbaih
Summary: The 2021 WHO classification has improved the diagnostic criteria for rare glial, neuronal, and glioneuronal tumors. These tumors present challenges in terms of diagnosis and therapeutic management, and require multimodal parameters for accurate diagnosis. Complete resection of the tumor is crucial for treatment, and targeting MAPK pathway abnormalities could be a promising approach for novel drugs.
Review
Oncology
Alessia Pellerino, Robert M. Verdijk, Lucia Nichelli, Nicolaus H. Andratschke, Ahmed Idbaih, Roland Goldbrunner
Summary: The 2021 WHO classification of the CNS Tumors recognizes Peripheral nerve sheath tumors (PNST) as a distinct group with specific characteristics and recommends diagnostic and therapeutic approaches based on limited evidence due to their rarity. Diagnosis is mainly determined by histological analysis and immunohistochemistry, with genetic analysis required for mosaic forms of NF1 and SPS. MRI is the preferred method for assessing tumor extent. Complete resection is the preferred treatment, balancing nerve preservation. Radiotherapy can be omitted in benign and NF-related tumors. Systemic therapy is recommended for incompletely resected plexiform neurofibromas/MPNSTs, with MEK inhibitors and anthracycline-based treatment being potential options. Clinical trials on MEK1-2 inhibitors and mTOR inhibitors are underway for plexiform neurofibromas and MPNST, respectively.
Article
Oncology
Tobias Kessler, Daniel Schrimpf, Laura Doerner, Ling Hai, Leon D. Kaulen, Jakob Ito, Martin van den Bent, Martin Taphoorn, Alba A. Brandes, Ahmed Idbaih, Julien Domont, Paul M. Clement, Mario Campone, Martin Bendszus, Andreas von Deimling, Felix Sahm, Michael Platten, Wolfgang Wick, Antje Wick
Summary: The EORTC-26101 study investigated the efficacy of combination therapy with bevacizumab and lomustine versus lomustine alone in progressive glioblastoma. Molecular data from DNA methylation arrays and panel sequencing were analyzed to assess prognostic biomarkers. The study found that MGMT promoter methylation and RTK1 classifier assignment were prognostic factors, while NF1 mutation may predict response to bevacizumab treatment.
CLINICAL CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Federica Fabro, Trisha V. Kers, Kate J. Feller, Cecile Beerens, Ioannis Ntafoulis, Ahmed Idbaih, Maite Verreault, Kate Connor, Archita Biswas, Manuela Salvucci, Jochen H. M. Prehn, Annette T. Byrne, Alice C. O'Farrell, Diether Lambrechts, Gonca Dilcan, Francesca Lodi, Ingrid Arijs, Andreas Kremer, Romain Tching Chi Yen, Miao-Ping Chien, Martine L. M. Lamfers, Sieger Leenstra
Summary: Chemotherapy using temozolomide is the standard treatment for glioblastoma, but temozolomide resistance remains a challenge. This study reveals the association between resistance and heterogeneity in glioblastoma, and identifies distinct phenotypic behaviors and gene expressions in response to temozolomide. The adaptability and heterogeneity of glioblastoma cells contribute to their ability to survive and develop resistance mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Clinical Neurology
P. Guillaume-Jugnot, N. Shor, F. Bielle, C. Zavanone, S. Barete, E. Maillart
REVUE NEUROLOGIQUE
(2023)