Review
Chemistry, Analytical
Yuan Su, Longjiao Zhu, Yifan Wu, Zihong Liu, Wentao Xu
Summary: This paper summarizes the factors influencing aptamer performance during the SELEX process and provides an advanced screening strategy for aptamers against bacterial whole-cell components. The binding sites between aptamers and bacterial whole-cell components are examined, and sequence sites are analyzed from an aptamer perspective. Additionally, the application status and prospects of bacterial aptamers are briefly described.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Agriculture, Multidisciplinary
Mengyue Liu, Fengling Yue, Qianqian Kong, Zhanli Liu, Yemin Guo, Xia Sun
Summary: Pathogenic bacteria can cause illness through contaminated food, and aptamer recognition factors have emerged as a rapid detection solution, offering advantages over antibodies. However, challenges in commercial availability remain.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Ming Xue, Qi Gao, Rui Yan, Lingping Liu, Ling Wang, Binyu Wen, Chongqing Wen
Summary: This study characterized the virulence factor repertoire of V. parahaemolyticus strain LC, which showed significant virulence to shrimp Litopenaeus vannamei. The results revealed the presence of specific genes associated with shrimp and human pathogenic strains, as well as unique mobile genetic elements-borne virulence factors. V. parahaemolyticus demonstrates host-specific virulence, contributing to the origin and spread of pathogenic factors.
Review
Biotechnology & Applied Microbiology
Yixin Xu, Xin Jiang, Yanhong Zhou, Ming Ma, Minjin Wang, Binwu Ying
Summary: This review provides a comprehensive overview of the latest development of SELEX technology and focuses on the applications of aptamer-based technologies in infectious diseases, such as targeted drug-delivery, treatments, and biosensors for diagnosing. The challenges and the future development in this field of clinical application will also be discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Na Ying, Yuan Wang, Xuefeng Song, Liguo Yang, Bo Qin, Yanqing Wu, Wenhong Fang
Summary: A new detection assay for Vibrio parahaemolyticus was introduced, utilizing aptamer combined with magnetic nanoparticles, hybridization chain reaction, and lateral flow nucleotide biosensor. It showed high specificity and fast detection time, making it suitable for low-resource laboratories or districts.
Article
Microbiology
Yiting Zhang, Hu Xing, Grigory Bolotnikov, Markus Kraemer, Nina Gotzmann, Uwe Knippschild, Ann-Kathrin Kissmann, Frank Rosenau
Summary: In this study, an enriched aptamer library was evolved for specific labeling and detection of Rikenella microfusus. The aptamer library showed high specificity and strong affinity, allowing efficient discrimination of R. microfusus from other bacteria in complex mixtures, including human stool samples. These results provide a new avenue for the development of aptamer-based biosensors for fast and reliable monitoring of R. microfusus.
Article
Chemistry, Analytical
Yunxia Hou, Liangliang Zhu, Hongshun Hao, Zuowei Zhang, Chao Ding, Gongliang Zhang, Jingran Bi, Shuang Yan, Guishan Liu, Hongman Hou
Summary: In this study, a photoelectrochemical (PEC) aptamer sensor was constructed using a layer-by-layer self-assembly method, with rare-earth doped Bi2WO6 and Ag2S as photoactive materials to enhance photocurrent responsiveness under visible light. The optimized experimental conditions showed high detection specificity and stability for Vibrio parahaemolyticus, indicating the potential of this method for rapid detection of other food-borne pathogenic bacteria.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Xiaoyan Jia, Jie Liu, Yanan Zhang, Xuyan Jiang, Junling Zhang, Jikui Wu
Summary: To improve the performance of whole-cell bacteria imprinted polymer-based sensors, we introduced D-tartaric acid (D-TA) as a dopant and pyrrole as a functional monomer to construct a D-TA/polypyrrole (PPy)-based bacteria imprinted polymer (DPBIP) sensor for Vibrio parahaemolyticus (VP) detection. D-TA doping synergistically accelerated template removal, improved bacteria affinity, and enhanced sensor sensitivity. The DPBIP sensor exhibited a linear range of 102-106 CFU mL-1, high selectivity, and good repeatability, with a recovery rate of 94.8%-105.3% in drinking water and oyster samples. Small functional molecules doping opens new possibilities for engineering high-performance BIP-based sensors, with potential applications in food safety.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Hui Yue, Jinri Chen, Xu Chen, Xiaoli Wang, Yuting Zhang, Nandi Zhou
Summary: This study utilized different magnetic beads-based SELEX methods to screen aptamers specific for Neu5Ac, and optimized the best aptamer through molecular simulation. A fluorescent biosensor constructed showed good specificity and interferents-resistance.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Analytical
Ziheng Hu, Yaqi Li, Gabriela Figueroa-Miranda, Simon Musall, Hangyu Li, Mateo Alejandro Martinez-Roque, Qinyu Hu, Lingyan Feng, Dirk Mayer, Andreas Offenhaeusser
Summary: Neurotransmitters control signal transmission in chemical synapses by being released from the pre-synaptic neuron and binding to receptors in the postsynaptic neuron. Measurement of neurotransmitter concentrations with high spatiotemporal resolution is essential for understanding brain function and dysfunction in neurological diseases. Aptamer based biosensors are a valuable tool in achieving this goal, offering advantages in tailoring receptors for specific tasks.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Engineering, Biomedical
Kauser Banu, Bhairab Mondal, Bhawana Rai, N. Monica, Raju Hanumegowda
Summary: Aptamer-based diagnostics platforms are gaining importance for detecting animal, human, plant, and environmental pathogens, as they offer rapid, user-friendly, sensitive, and selective detection. This review provides a brief overview of the development of various aptamer-based platforms and their applicability for sensitive pathogen detection.
BIOCYBERNETICS AND BIOMEDICAL ENGINEERING
(2022)
Article
Agriculture, Multidisciplinary
Jinri Chen, Xin Chen, Yuting Zhang, Xiaoli Wang, Nandi Zhou
Summary: A single-stranded DNA aptamer specific for 6'-sialyllactose (6'-SL) was screened and optimized. It was used to synthesize bio-dots along with aminobenzene boric acid functionalized carbon dots, forming a 6'-SL fluorescent biosensor. The biosensor exhibited a linear detection range of 10 μM to 5 mM and a detection limit of 0.9 μM. The aptamer and biosensor have potential applications in 6'-SL detection due to their time-saving, high efficiency, and simplicity in actual sample detection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Nandi Zhou, Jinri Chen, Xin Chen, Xiaoli Wang, Yuting Zhang
Summary: A single-stranded DNA aptamer specific for 6'-sialyllactose (6'-SL) was screened and modified for the construction of a sensitive biosensor. The truncated aptamer Apt9-1 showed higher affinity to 6'-SL than the original aptamer Apt9. The biosensor, utilizing Apt9-1 and aminobenzene boric acid functionalized carbon dots, exhibited a linear detection range of 10 μM to 5 mM and a detection limit of 0.9 μM.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Hu Xing, Ann-Kathrin Kissmann, Heinz Fabian Raber, Markus Kramer, Valerie Amann, Kathrin Kohn, Tanja Weil, Frank Rosenau
Summary: This study reports on the evolution of a high-affinity aptamer library using FluCell-SELEX technology, which can specifically recognize, bind and label P. distasonis, and accurately distinguish P. distasonis from other gut bacteria in mixtures.
Article
Immunology
Kattapuni Suresh Prithvisagar, Ballamoole Krishna Kumar, Toshio Kodama, Praveen Rai, Tetsuya Iida, Iddya Karunasagar, Indrani Karunasagar
Summary: This study analyzed the genomes of Vibrio parahaemolyticus isolates from moribund shrimps in India, revealing significant genetic diversity without the classical PirAB features associated with the AHPND strains. The presence of various virulence attributes, including toxins and secretion systems, highlights the potential evolution of environmental V. parahaemolyticus into pathogens of aquaculture species. Monitoring of V. parahaemolyticus genomes is necessary to study the emergence of new variants in shrimp aquaculture and implement biosecurity measures to prevent disease outbreaks.