期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 430, 期 4, 页码 524-536出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2017.10.021
关键词
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资金
- National Institutes of Health [R00 GM086471, R01 GM112735]
- Shaw Scientist Award
- Beckman Young Investigator Award
- University of Wisconsin Madison
- Wisconsin Alumni Research Foundation
- Department of Biochemistry
of the spliceosome is targeted for additional post-transcriptional modifications in response to cellular stress. Uridines 56 and 93 are both modified to pseudouridines (psi) during nutrient deprivation, while U56 is also pseudouridylated during heat shock. Both positions are located within stem II, which must toggle between two mutually exclusive structures during splicing. Stem IIa forms during spliceosome assembly, and stem IIc forms during the catalytic steps. We have studied how uridine 56 and 93 pseudouridylation impacts conformational switching of stem II. Using single-molecule Forster resonance energy transfer, we show that psi 56 dampens conformational dynamics of stem II and stabilizes stem IIc. In contrast, psi 93 increases dynamics of non-stem IIc conformations. Pseudouridylation impacts conformational switching of stem II by Mg2+ or the U2 protein Cus2; however, when Mg2+ and Cus2 are used in combination, the impacts of pseudouridylation can be suppressed. These results show that stress-induced post-transcriptional modification of U56 and U93 alters snRNA conformational dynamics by distinct mechanisms and that protein and metal cofactors of the spliceosome alter how snRNAs respond to these modifications. (C) 2017 Elsevier Ltd. All rights reserved.
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