期刊
JOURNAL OF IMMUNOLOGY
卷 200, 期 8, 页码 2905-2914出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1701625
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-
类别
资金
- National Institutes of Health [R01DK103576]
A set of coordinated interactions between gut microbiota and the immune cells surveilling the intestine play a key role in shaping local immune responses and intestinal health. Gpr109a is a G protein coupled receptor expressed at a very high level on innate immune cells and previously shown to play a key role in the induction of colonic regulatory T cells. In this study, we show that Gpr109a(-/-)Rag1(-/-) mice exhibit spontaneous rectal prolapse and colonic inflammation, characterized by the presence of an elevated number of IL-17 producing Ror gamma t(+) innate lymphoid cells (ILCs; ILC3). Genetic deletion of Ror gamma t alleviated the spontaneous colonic inflammation in Gpr109a(-/-)Rag1(-/-) mice. Gpr109a-deficient colonic dendritic cells produce higher amounts of IL-23 and thereby promote ILC3. Moreover, the depletion of gut microbiota by antibiotics treatment decreased IL-23 production, ILC3, and colonic inflammation in Gpr109a(-/-)Rag1(-/-) mice. The ceca of Gpr109a(-/-)Rag1(-/-) mice showed significantly increased colonization by members of Bacteroidaceae, Porphyromonadaceae, Prevotellaceae, Streptococcaceae, Christensenellaceae, and Mogibacteriaceae, as well as IBD-associated microbiota such as Enterobacteriaceae and Mycoplasmataceae, compared with Rag1(-/-) /mice, housed in a facility positive for Helicobacter and murine norovirus. Niacin, a Gpr109a agonist, suppressed both IL-23 production by colonic DCs and ILC3 number in a Gpr109a-dependent manner. Collectively, our data present a model suggesting that targeting Gpr109a will be potentially beneficial in the suppression of IL-23 mediated immunopathologies.
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