期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 456, 期 -, 页码 54-60出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2018.02.012
关键词
Immunodetection; WSSV; Monoclonal antibodies; Cocktail; FTA
资金
- Karnataka Veterinary Animal and Fisheries Sciences University, Bidar, Karnataka, India
A panel of four monoclonal antibodies (C-05, C-14, C-38 and C-56) specific to VP28 of White spot syndrome virus (WSSV) were evaluated individually and in cocktail to increase sensitivity of the Flow Through Assay (FTA) for detection of the virus. Recombinant VP28 and semi purified WSSV was used as antigen for evaluation. Out of the total 11 cocktails and four individual of MAbs, 2 MAb cocktails C-05 + C-56 and C-14 + C-56 exhibited highest sensitivity in the FTA. The two MAb cocktail were 100 times more sensitive than 1-step PCR and nearly equivalent to 2-step PCR for the detection of WSSV. The detection limit of WSSV by MAb cocktail increased by two fold compared to the single MAb C-05 currently being used in (FTA).
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