Article
Plant Sciences
Uri Karniel, Nastacia Adler Berke, Varda Mann, Joseph Hirschberg
Summary: Regulation of carotenoid synthesis in tomato fruit involves specific perturbations of carotenoid biosynthesis downstream to phytoene, which reactivates the chloroplast-specific PSY2 in fruit chromoplasts. This finding provides a plausible molecular explanation for the mutation tangerine in the gene carotenoid isomerase over yellow flesh.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Fanliang Meng, Haoran Liu, Songshen Hu, Chengguo Jia, Min Zhang, Songwen Li, Yuanyuan Li, Jiayao Lin, Yue Jian, Mengyu Wang, Zhiyong Shao, Yuanyu Mao, Lihong Liu, Qiaomei Wang
Summary: The study investigates how brassinosteroids (BRs) regulate fruit ripening in tomato and their interactions with ethylene. It is found that BR treatment and increased endogenous BR contents promote ethylene production and fruit ripening. The BR signaling regulators SlBZR1 and SlBES1 redundantly act in fruit softening. Knocking out SlBZR1 inhibits ripening through transcriptional reprogramming. SlBZR1 directly targets ethylene and carotenoid biosynthetic genes, contributing to ethylene burst and carotenoid accumulation for normal ripening and quality formation. Overall, SlBZR1 is highlighted as a master regulator of tomato fruit ripening with potential for quality improvement and carotenoid fortification.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Xuedong Yang, Xuelian Zhang, Youxin Yang, Hui Zhang, Weimin Zhu, Wen-Feng Nie
Summary: The study utilized the CRISPR/Cas9 gene-editing system to generate a sl_hta9 sl_hta11 double mutant, designated sl_h2a.z, which significantly reduced the fresh weight of tomato fruits. mRNA-seq results showed that Sl_H2A.Z dysfunction has profound effects on genome-wide gene expression in tomato fruits at different developmental stages, with a ripening-dependent correlation between Sl_H2A.Z and gene expression regulation. Additionally, the upregulation of key genes in the carotenoid biosynthesis pathway in the late ripening stages of sl_h2a.z mutant fruits was consistent with increased carotenoid contents, revealing a role of Sl_H2A.Z in carotenoid regulation and providing insights into histone variants' regulation of tomato fruit quality.
HORTICULTURE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jun-E Guo
Summary: Fruit development and ripening is a complex process regulated by plant hormones, transcription factors, and epigenetic modifications. This study focuses on the role of histone deacetylation gene SlHDT1 in tomato fruit ripening and found that it acts as a negative regulator, influencing ethylene and carotenoid biosynthesis.
Article
Plant Sciences
Yun Liu, Junli Ye, Man Zhu, Ross G. Atkinson, Yingzi Zhang, Xiongjie Zheng, Jiao Lu, Zhen Cao, Jun Peng, Chunmei Shi, Zongzhou Xie, Robert M. Larkin, Niels J. Nieuwenhuizen, Charles Ampomah-Dwamena, Chuanwu Chen, Rui Wang, Xiaozhou Luo, Yunjiang Cheng, Xiuxin Deng, Yunliu Zeng
Summary: This study identified the core proteome of chromoplast plastoglobules (CPGs) and revealed the role of the nonpolar microenvironment in carotenoid accumulation and storage in citrus chromoplasts.
Review
Plant Sciences
Yucheng Ming, Libo Jiang, Dongchao Ji
Summary: This review discusses the recent advances in epigenetic regulation of tomato fruit ripening, including DNA methylation, N6-Methyladenosine mRNA modification, histone demethylation/deacetylation, and non-coding RNA. This research contributes to a better understanding of the regulatory mechanisms governing tomato fruit ripening and provides new insights for precise modulation of these mechanisms.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Applied
Can Yang, Jian Qin, Fei Xie, Kun Zhou, Wanpeng Xi
Summary: This study investigated the effects of red, blue, and white light-transmittance bagging on carotenoid metabolism during 'Moro' blood orange ripening. The results showed that red light-transmittance bagging increased the esterification of easily absorbed xanthophylls and promoted carotenoid accumulation.
Review
Plant Sciences
Zhiya Liu, Xuetong Wu, Huwei Liu, Meiling Zhang, Weibiao Liao
Summary: Fruit ripening is regulated by transcription factors, plant hormone ethylene, and DNA methylation. DNA methylation affects fruit ripening by transferring methyl groups. Tomato fruit ripening is dynamically regulated by DNA methylation and demethylation, but the specific molecular mechanism is still unclear.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Agronomy
Bruna Orsi, Ivan Sestari, Ana Paula Preczenhak, Magda Andreia Tessmer, Mayara Adja da Silva Souza, Neuza Mariko Aymoto Hassimotto, Ricardo Alfredo Kluge
Summary: Variations in carotenoid biosynthesis genes found in tomato mutants alter the accumulation of carotenoids and impact fruit ripening, color, antioxidant capacity, and nutritional composition.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Changan Zhu, Shaofang Wu, Ting Sun, Zhiwen Zhou, Zhangjian Hu, Jingquan Yu
Summary: Rosmarinic acid (RA) treatment in postharvest significantly delayed ripening, reduced ethylene production, inhibited color change, promoted amino acid accumulation, and enhanced antioxidant activity in tomato fruits. These findings indicate a novel function of RA in fruit ripening, offering an attractive strategy to manage and improve the quality of tomato fruits.
Article
Plant Sciences
Fabian Ries, Heinrich Lukas Weil, Claudia Herkt, Timo Muehlhaus, Frederik Sommer, Michael Schroda, Felix Willmund
Summary: The integration of nuclear- and chloroplast-encoded polypeptides into chloroplasts is crucial for metabolic processes. The chloroplast chaperonin machinery is important for protein folding, but its role in chloroplasts is still unclear due to limited knowledge about its interactors.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Bin Liang, Yufei Sun, Juan Wang, Yu Zheng, Wenbo Zhang, Yandan Xu, Qian Li, Ping Leng
Summary: The study revealed the crucial role of tomato ABA co-receptor type 2C phosphatase SlPP2C3 in regulating fruit ripening and fruit glossiness. SlPP2C3 interacts selectively with ABA receptors and kinase, negatively regulates ABA signaling, and affects the onset of fruit ripening.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Guoxiang Jiang, Zhiwei Li, Xiaochun Ding, Yijie Zhou, Hongmei Lai, Yueming Jiang, Xuewu Duan
Summary: A WUSCHEL-related homeobox transcription factor (TF), SlWOX13, regulates tomato fruit ripening via ethylene synthesis and signaling, as well as transcriptional regulation of key ripening-related TFs.
Article
Plant Sciences
Yusuke Kamiyoshihara, Yuki Achiha, Shin Ishikawa, Shinji Mizuno, Hitoshi Mori, Akira Tateishi, Donald J. Huber, Harry J. Klee
Summary: Ethylene receptors function in heteromeric complexes across subfamilies in tomato fruit, providing insights into the regulation of ethylene signaling during tomato fruit ripening. Tomato receptor members form heteromeric complexes to fine-tune signal output to the downstream pathway, similar to the Arabidopsis system but with some differences.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Chunoti Changwal, Tushita Shukla, Zakir Hussain, Neera Singh, Abhijit Kar, Virendra P. Singh, M. Z. Abdin, Ajay Arora
Summary: High endogenous SA is associated with low ET evolution and high SL, while high lycopene content is correlated with high SA levels. Total antioxidants, PG, and PME decrease with fruit development, while lipid peroxidation increases. SlPAL4 and SlPAL6 genes play significant but opposite roles in regulating fruit ripening.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Oncology
Jose Javier Ruiz, Monica Marro, Ismael Galvan, Jose Bernabeu-Wittel, Julian Conejo-Mir, Teresa Zulueta-Dorado, Ana Belen Guisado-Gil, Pablo Loza-Alvarez
Summary: Raman spectroscopy combined with multivariate analysis has the potential to non-invasively diagnose skin tumors by characterizing their pigment composition. Differences in DHICA content allow for the accurate classification of dysplastic lesions in an objective, efficient, and cost-effective manner.
Correction
Plant Sciences
M. Aguila Ruiz-Sola, M. Victoria Barja, David Manzano, Briardo Llorente, Bert Schipper, Jules Beekwilder, Manuel Rodriguez-Concepcion
Article
Biochemistry & Molecular Biology
Vishnu Balaji, Leonie Mueller, Robin Lorenz, Eva Kevei, William H. Zhang, Ulises Santiago, Jan Gebauer, Ernesto Llamas, David Vilchez, Carlos J. Camacho, Wojciech Pokrzywa, Thorsten Hoppe
Summary: The ubiquitin ligase CHIP plays a crucial role in substrate selection and processing through its dimer-monomer transition and autoubiquitylation mechanisms, allowing it to respond and regulate cellular stress.
Article
Plant Sciences
Luca Morelli, Salvador Torres-Montilla, Gaetan Glauser, Venkatasalam Shanmugabalaji, Felix Kessler, Manuel Rodriguez-Concepcion
Summary: The accumulation of phytonutrients in plant tissues can be enhanced by stimulating biosynthesis and providing suitable structures. Artificial chromoplasts induced by the bacterial protein crtB can accumulate high levels of carotenoids and other health-related isoprenoids. Increasing plastoglobule proliferation and reactive oxygen species production can further promote the accumulation of these metabolites and the conversion from chloroplasts to chromoplasts.
Article
Biochemistry & Molecular Biology
Luca Morelli, Garcia Romanach Laura, Gaetan Glauser, Venkatasalam Shanmugabalaji, Felix Kessler, Manuel Rodriguez-Concepcion
Summary: Artificial leaf chromoplasts can increase the accumulation and storage of vitamin E in plants. In addition, this method also leads to higher levels of other related compounds such as carotenoids and phylloquinones.
Article
Biochemistry & Molecular Biology
Xueni Di, David Ortega-Alarcon, Ramu Kakumanu, Javier Iglesias-Fernandez, Lucia Diaz, Edward E. K. Baidoo, Adrian Velazquez-Campoy, Manuel Rodriguez-Concepcion, Jordi Perez-Gil
Summary: Isoprenoids, essential metabolites for all living organisms, are derived from IPP and DMAPP produced by the MEP pathway. Research shows that IPP and DMAPP feedback-regulate the activity of DXS, a key enzyme in the MEP pathway. The study provides insights into the mechanism and regulation of the MEP pathway.
PLANT COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Luca Morelli, Manuel Rodriguez-Concepcion
Summary: Plant carotenoids have multiple functions and benefits, and their profile in crops can be improved through biotechnology. However, challenges such as regulatory constraints and off-target effects need to be addressed. There is also a need to explore ways to mitigate the negative impact of altered carotenoid contents on photosynthesis. Additionally, the stability and bioaccessibility of carotenoid-enriched products should be confirmed. Overcoming these challenges will lead to advancements in carotenoid biotechnology and biofortification.
PLANT COMMUNICATIONS
(2023)
Review
Plant Sciences
Jaime F. F. Martinez-Garcia, Manuel Rodriguez-Concepcion
Summary: Shade tolerance is an ecological concept that influences the organization, structure, functioning, and dynamics of plant communities. The molecular mechanisms of shade tolerance are not well understood, but there is a good understanding of how plants respond to vegetation proximity. Comparative studies have shown that shade tolerance is implemented by components similar to those involved in elongation response, but with differential molecular properties.
Article
Biochemical Research Methods
Wayne Zita, Venkatasalam Shanmugabalaji, Miguel Ezquerro, Manuel Rodriguez-Concepcion, Felix Kessler, Gaetan Glauser
Summary: In this study, a method was developed to quantify GGPP and GGP in tomato fruit using UHPLC-MS/MS. The validity of the method was confirmed by analyzing GGPP contents in wild-type tomatoes and mutants defective in GGPP production. Sample preparation was also shown to be crucial in preventing GGPP hydrolysis and minimizing its conversion to GGP.
Article
Cell Biology
Norma Medina-Villalobos, Remy Avila, Maria Marsal, Jordi Andilla, Pablo Loza-alvarez, Mario Miguel Ojeda-Ramirez, Elisa Tamariz
Summary: Highly focused near-infrared (NIR) lasers can induce cell protrusions in fibroblasts and neurons, but the biochemical and biophysical effects and optimal stimulation protocols are still unknown. In this study, intermittent NIR laser radiation was used to investigate the direction and velocity of cell protrusions. The results showed that the orientation and advance of cell protrusions continue after the laser is turned off, but the increase in velocity stops. The study also revealed that NIR irradiation promotes the recruitment of actin, leading to filament formation at the induced cell protrusions.
Article
Biodiversity Conservation
Edison Cardona-Medina, Marisa Santos, Rubens Nodari, Damaso Hornero-Mendez, Arnau Peris, Darren C. J. Wong, Jose Tomas Matus, Manuel Rodriguez-Concepcion
Summary: This article investigates the chemical identification, biosynthesis, and potential function of an orange pigment in the roots of Escobedia grandiflora, a medicinal hemiparasite found in Central and South America. The research also explores the traditional medicinal value of the plant.
PLANTS PEOPLE PLANET
(2023)
Article
Plant Sciences
Miguel Ezquerro, Esteban Burbano-Erazo, Manuel Rodriguez-Concepcion
Summary: Carotenoids are important compounds in plants that play crucial roles in photoprotection and phytohormone production. By editing the PSY1 and PSY2 genes in tomato, it was found that PSY1 and PSY2 have different contributions to carotenoid production in different tissues. PSY2 is the main isoform for carotenoid biosynthesis in leaf chloroplasts, while PSY1 is important in response to high light and in fruit chromoplasts. Furthermore, the study revealed that fruit growth is controlled by ABA specifically produced from PSY1-derived carotenoid precursors, while PSY2 is associated with ABA synthesis in seeds and salt-stressed roots.
Article
Plant Sciences
Xueni Di, Manuel Rodriguez-Concepcion
Summary: Isoprenoids are diverse metabolites in plants, and the DXS enzyme is the key rate-determining enzyme in the MEP pathway. In tomato, three genes encode DXS-like proteins, which have distinct expression patterns and physiological roles. Our study confirms the DXS activity of SlDXS1 and SlDXS2, while SlDXS3 lacks DXS activity. Co-localization and co-immunoprecipitation experiments suggest that SlDXS1 and 2 may form heterodimers in plastidial speckles.
Article
Plant Sciences
Ariadna Iglesias-Sanchez, Juan Navarro-Carcelen, Luca Morelli, Manuel Rodriguez-Concepcion
Summary: The interaction between FIBRILLIN6 and phytoene synthase promotes carotenogenesis in bacteria and chloroplasts.
Article
Cell Biology
Ernesto Llamas, Seda Koyuncu, Hyun Ju Lee, Markus Wehrmann, Ricardo Gutierrez-Garcia, Nick Dunken, Nyasha Charura, Salvador Torres-Montilla, Elena Schlimgen, Amrei M. Mandel, Erik Boelen Theile, Jan Grossbach, Prerana Wagle, Jan-Wilm Lackmann, Bernhard Schermer, Thomas Benzing, Andreas Beyer, Pablo Pulido, Manuel Rodriguez-Concepcion, Alga Zuccaro, David Vilchez
Summary: This study found that Arabidopsis suppresses polyQ aggregation through chloroplast proteostasis, unlike in animal models. Synthetic plant proteins, such as SPP, prevent disease-related protein aggregation and neurodegeneration in human cell and nematode models.