Article
Immunology
Dakang Hu, Wenjie Chen, Qi Zhang, Meng Li, Zehua Yang, Yong Wang, Yunkun Huang, Gang Li, Dongxing Tian, Pan Fu, Weiwen Wang, Ping Ren, Qing Mu, Lianhua Yu, Xiaofei Jiang
Summary: This study investigated the epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP) in mainland China. Molecular determination of CR-HvKP and Hv-CRKP showed biases compared to mouse lethality test, with the exact prevalence of CR-HvKP being less than 1.0% and that of Hv-CRKP even lower.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Courtney L. Luterbach, Liang Chen, Lauren Komarow, Belinda Ostrowsky, Keith S. Kaye, Blake Hanson, Cesar A. Arias, Samit Desai, Jason C. Gallagher, Elizabeth Novick, Stephen Pagkalinawan, Ebbing Lautenbach, Glenn Wortmann, Robert C. Kalayjian, Brandon Eilertson, John J. Farrell, Todd McCarty, Carol Hill, Vance G. Fowler, Barry N. Kreiswirth, Robert A. Bonomo, David van Duin
Summary: This study evaluated the clustering of carbapenem-resistant Klebsiella pneumoniae (CRKp) among patients in US hospitals and identified widespread intrasystem and intersystem transmission. Different methods for assessing genetic similarity resulted in only minor differences in interpretation.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Irina Magdalena Dumitru, Mirela Dumitrascu, Nicoleta Dorina Vlad, Roxana Carmen Cernat, Carmen Ilie-Serban, Aurelia Hangan, Raluca Elena Slujitoru, Aura Gherghina, Corina Mitroi-Maxim, Licdan Curtali, Dalia Sorina Carp, Anca Dumitrescu, Romelia Mitan, Rodica Lesanu, Sorin Rugina
Summary: Infections with carbapenem-resistant Enterobacteriaceae pose a significant challenge in healthcare settings, especially with CRKP being resistant to the majority of available antimicrobial agents and leading to high rates of morbidity and mortality. A recent report of patients in an ICU with COVID-19 who developed invasive infections due to CP-Kp strains highlights the importance of rigorous antibiotic administration programs in intensive care units, especially for high-risk populations like the elderly.
Article
Microbiology
Feiyang Zhang, Qin Li, Jiawei Bai, Manlin Ding, Xiangjin Yan, Guangxi Wang, Baoli Zhu, Yingshun Zhou
Summary: This study revealed that 8.39% of Klebsiella pneumoniae strains were amikacin-heteroresistant, with potential association with increased expression of aminoglycoside resistance genes. The majority of heteroresistant phenotypes were unstable, with partial or full reversion of minimal inhibitory concentrations to the level of susceptibility.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Gabriel Mendes, Maria Leonor Santos, Joao F. Ramalho, Aida Duarte, Catia Caneiras
Summary: Hypervirulent and carbapenem-resistant pathotypes of Klebsiella pneumoniae have emerged as distinct evolutionary types with significant impact on public health. However, there is concern over the convergence of high-risk clones that pose a challenge to available therapeutic options. This review aims to describe the worldwide distribution of virulence factors among carbapenem-resistant highly virulent or hypervirulent K. pneumoniae strains and their interactions with carbapenemase production and sequence types. Urgent understanding of these strains is necessary for effective response as they increasingly replace classical strains in healthcare settings.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Xuemei Yang, Qiaoling Sun, Jiaping Li, Yu Jiang, Yi Li, Jianping Lin, Kaichao Chen, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Summary: The epidemiological features and potential threat to human health of the newly emerged carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) are currently unknown. This study analyzed 784 CRKP strains collected from three hospitals in China and found that the proportion of CRKP strains among clinical K. pneumoniae strains increased sharply. A significant number of these CRKP strains carried a virulence-encoding plasmid, but only a small percentage exhibited a hypervirulent phenotype. Genetic markers were not strongly correlated with hypervirulent phenotypes, indicating that additional factors may contribute to the hypervirulence of CRKP. The transmission of these CRKP strains in China likely involved multiple clones of ST11 and the major mechanism of carbapenem resistance was the carriage of IncFII pSWU01-like, bla (KPC-2)-bearing plasmids. These findings highlight the rapid increase in prevalence of CRKP strains carrying virulence plasmids in China and the need for better definition and screening of truly hypervirulent CR-HvKP strains in clinical settings.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Biochemistry & Molecular Biology
Hao Guo, Yuye Wu, Lirong Li, Jianfeng Wang, Juan Xu, Fang He
Summary: The study investigated the global dissemination trends of carbapenem-resistant Klebsiella pneumoniae (CRKP) strains that co-carry multiple carbapenemases. A CRKP strain, KP424, co-carrying blaNDM-1 and blaKPC-2, was identified and sequenced. Genome sequences of CRKP strains carrying multiple carbapenemase genes were retrieved from the NCBI GenBank database. The prevalence of CRKP strains co-carrying two carbapenemase genes has significantly increased over time, with strains carrying both blaNDM and blaOXA-48-like being the most common worldwide. Further research is needed to understand the mechanisms of multiple carbapenemase gene occurrence and prevent their global dissemination.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Public, Environmental & Occupational Health
Na Huang, Huaiyu Jia, Beibei Zhou, Cui Zhou, Jianming Cao, Wenli Liao, Shixing Liu, Lingbo Wang, Liqiong Chen, Lijiang Chen, Tieli Zhou, Jianzhong Ye
Summary: This study investigated the clinical and microbiological characteristics of Klebsiella pneumoniae meningitis, revealing an association between antimicrobial resistance, virulence, and patient prognosis. The high fatality of meningitis caused by hypervirulent carbapenem-resistant K. pneumoniae strains is particularly concerning.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Immunology
Jihui Chen, Yu Yang, Huimin Yao, Shuhong Bu, Lixia Li, Fang Wang, Feng Chen, Huijuan Yao
Summary: This study analyzed the clinical information of adult patients with carbapenem-resistant Klebsiella pneumoniae (CRKP) infection, established a nomogram for predicting mortality risk, and determined the effectiveness of different antimicrobial regimens. The results can help clinicians identify high-risk adult patients with CRKP infection, improve treatment outcomes, and reduce mortality.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Luana Soares de Moraes, Gerusa Luciana Gomes Magalhaes, Joao Gabriel Material Soncini, Marsileni Pelisson, Marcia Regina Eches Perugini, Eliana Carolina Vespero
Summary: This study evaluated clinical data from 107 patients with bloodstream infection caused by Klebsiella pneumoniae and conducted phenotypic and molecular analyses on 50.5% (54/107) of samples that exhibited resistance to carbapenems. The bla(NDM) gene was present in 90.4% (49/54) of these samples, bla(KPC) gene was found in one sample, and 7.4% (4/54) of samples showed no carbapenemase gene. The study identified four main clones and found that 11 samples were not genetically related. The median age of the patients was 58 years old, with a majority of males (60.7%). Comparing patients with and without carbapenem resistance, several factors including ICU stay, renal failure, previous antimicrobial use, Charlson's comorbidity index, invasive procedures, and death showed statistically significant differences. Additionally, renal failure, liver failure, and BSI with extensive-drug resistance (XDR) or pan-drug resistance (PDR) were predictors of increased mortality. The study concluded that patients with carbapenem-resistant pneumonia, regardless of additional resistance to polymyxins, experienced higher mortality rates.
MICROBIAL PATHOGENESIS
(2022)
Article
Infectious Diseases
Lijia Ni, Zhixian Zhang, Rui Shen, Xiaoqiang Liu, Xuexue Li, Baiji Chen, Xiquan Wu, Hongyu Li, Xiaoying Xie, Songyin Huang
Summary: Disinfectant resistance is a serious concern for hospital infection control due to the extensive use of disinfectants. Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a common and challenging pathogen in nosocomial infections. This study found that CRKP strains exhibited extensive resistance to various clinical disinfectants.
Article
Infectious Diseases
Tao Wang, Xiaojun Wang, Suming Chen, Jie Zhu, Zhichen Zhu, Fen Qu, Liang Chen, Hong Du
Summary: This study investigated the clinical emergence of colistin-heteroresistant, hypervirulent, and multidrug-resistant Klebsiella pneumoniae, and characterized the underlying molecular mechanisms. The results showed the detection of two colistin-heteroresistant isolates, which exhibited similar survival rates as the hypervirulent reference strain. Additionally, overexpression of efflux pump genes was involved in mediating colistin heteroresistance.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Xiaoxue Liang, Ping Chen, Baoguo Deng, Feng-Hui Sun, Yongqiang Yang, Yanxian Yang, Ruowen He, Mingyang Qin, Yiping Wu, Fan Yang, Guo-Bao Tian, Min Dai
Summary: This study compared the antimicrobial resistance, virulence, clinical characteristics, and risk factors between carbapenem-resistant K. pneumoniae (CRKP) and carbapenem-susceptible K. pneumoniae (CSKP) isolates from patients with bloodstream infections (BSIs) in China. The results demonstrated that CRKP isolates were more drug-resistant, more virulent, and associated with poorer outcomes compared to CSKP isolates.
INFECTION AND DRUG RESISTANCE
(2022)
Review
Medicine, General & Internal
Wioletta Medrzycka-Dabrowska, Sandra Lange, Katarzyna Zorena, Sebastian Dabrowski, Dorota Ozga, Lucyna Tomaszek
Summary: The study highlights the importance of paying attention to carbapenem-resistant Klebsiella pneumoniae infections in patients with COVID-19. Rational antibiotic therapy should be used to prevent the increase of bacterial resistance, along with continuous monitoring and surveillance of hospital infections caused by multidrug-resistant organisms.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Microbiology
Wei Liu, Guang Chen, Keke Dou, Bingcheng Yi, Danyang Wang, Qihui Zhou, Yunbo Sun
Summary: The study investigates the antibacterial activity of eugenol (EG) on carbapenem-resistant Klebsiella pneumoniae (CRKP) and the underlying mechanisms. It is found that EG exhibits dose-dependent inhibitory effects on CRKP by inducing reactive oxygen species (ROS) and reducing glutathione, leading to membrane rupture and leakage of bacterial cytoplasmic components. Moreover, EG can also disrupt the integrity of bacterial biofilm.
FRONTIERS IN MICROBIOLOGY
(2023)