Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiao-wei Zhang, Yu-ting Chen, Xue Feng, Lu-ya Li, Ke-wei Song, Yu-peng Sun, Guo-hua Zhang, Lan-tong Zhang
Summary: In this study, the metabolism of celastrol in vivo and in vitro was comprehensively characterized using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. A total of 43 metabolites were identified and characterized, and it was found that the biotransformation capacity of the intestinal tract exceeded that of the liver. These findings provide a basis for understanding the pharmacological mechanisms of celastrol and have potential implications for clinical applications.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Nutrition & Dietetics
Jian Li, Junmei Ma, Qiang Li, Sufang Fan, Lixin Fan, Hongyu Ma, Yan Zhang, Lei Zheng
Summary: This research demonstrates the development of a simple, sensitive, and accurate method for simultaneous determination of 35 free amino acids in tea samples using UPLC-Q-TOF/MS. The method showed good linearity, low limits of detection, and high recovery rates.
FRONTIERS IN NUTRITION
(2021)
Article
Food Science & Technology
Jian Li, Junmei Ma, Yan Zhang, Lei Zheng
Summary: A rapid method was developed for the determination of 19 polyphenols in tea using ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). The method exhibited simplicity, rapidity, and accuracy, making it suitable for the rapid screening and quantitative analysis of polyphenols in tea.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Chemistry, Analytical
Zhuoyu Fan, Jiao Guan, Lele Li, Yue Cui, Xinmiao Tang, Xiaoying Lin, Guanghai Shen, Bo Feng, Heyun Zhu
Summary: In this study, an ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry method was developed to separate and identify the chemical components of Huangqi Guizhi Wuwu decoction (HGWWD). A total of 81 compounds were identified and characterized. The results showed that monoterpenoids, flavonoids, organic acids, triterpenes, amino acids, gingerols, alkaloids, and glycosides were the main chemical components of HGWWD.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Xiaowei Zhang, Xinpeng Diao, Luya Li, Yuqian Zhang, Man Liao, Guohua Zhang, Lantong Zhang
Summary: This study analyzed the metabolic profile of ginkgolide B using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry, and identified a total of 53 different metabolites. The biotransformation route of ginkgolide B was determined, providing useful information for further research on its pharmacology and mechanism.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Johannes Wellmann, Conny Traenkner, Nicolas Dostert, Esther-Corinna Schwarze, Silke Hillebrand, Jakob P. Ley, Peter Winterhalter
Summary: A workflow for comprehensive semi-polar metabolite profiling was developed and validated using liquid chromatography coupled with electrospray ionization ion mobility quadrupole time-of-flight mass spectrometry. This workflow has the capability to distinguish different cultivars of Hydrangea macrophylla ssp. serrata and obtain a list of metabolites responsible for the differentiation. Suspect screening of phenyldihydroisocoumarins provides valuable information for the biosynthesis of phyllodulcin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yuxuan Li, Yue Cui, Lele Li, Xiaoyin Lin, Xinxin Zhou, Heyun Zhu, Bo Feng
Summary: Poloxamer 124 is a commonly used pharmaceutical excipient, and this study successfully developed a method to quantify it in rat plasma. The results indicated that Poloxamer 124 can be rapidly absorbed and eliminated through caudal vein injection, providing valuable information for further research on this substance.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Shuaishuai Fan, Baolin Li, Yurou Tian, Wei Feng, Liying Niu
Summary: In this study, a novel method was established to analyze the chemical constituents of Yangwei decoction. A total of 226 compounds were identified, providing a robust foundation for future research.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Applied
Suo Decheng, fan xia, Xiao Zhiming, Wei Shulin, Wang Shi, Wang Peilong
Summary: The method developed enables rapid screening and quantification of progesterone and progestins in milks using UHPLC QE HF HRMS. It meets the validation requirements for veterinary drug residue detection in the EU and China, and has been successfully applied to detect trace levels of progesterone and monitor residual progestins in real milk.
Article
Biochemical Research Methods
Qi Dong, Na Hu, Huilan Yue, Honglun Wang, Jinliang Ku
Summary: This study established a method for rapid identification of glycosidase inhibiting compounds from quinoa bran, and identified a variety of flavonoids and triterpenoid saponins that may bind to alpha-glycosidase using SPR and LC-MS. Analysis of interaction kinetics and molecular docking supported their alpha-glucosidase-inhibiting activity, indicating the potential of quinoa bran as a natural source of such inhibitors.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Guochu Huang, Sheng Xie, Meng Wang, Dewen Mao, Guye Huang, Jingjing Huang, Xirong Liu, Rongzhen Zhang, Jiacheng Xie, Liang Jiang Huang, Chen Cheng, Fan Yao, Yu Zhong, Long Lin, Chun Yao
Summary: This study used GC-TOFMS and UPLC-QTOFMS metabonomic analytical techniques to analyze the serum metabolite profiling of HBV-induced liver cirrhosis patients with MHE and identified potential biomarkers for MHE. The study found differential metabolites in the MHE group compared to the NC group, as well as in the DS group compared to the NC group. These findings suggest that metabonomic analysis combined with pattern recognition analysis has the potential for early diagnosis of MHE.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Chemistry, Analytical
Ting Chen, Renyuan Zhu, Wen Zhang, Jian Li, Guoyu Qiu, Fuxiang Wu, Yanli Xu, Min Chen, Pengfei Qi
Summary: This study presents an analytical method for rapidly screening multiple pesticide residues in Lilii Bulbus using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The method is validated and shows effective results in detecting pesticide residues in Lilii Bulbus.
ANALYTICAL METHODS
(2023)
Article
Biochemical Research Methods
Huibo Lei, Jiayun Xin, Yanhui Lv, Wei Chen, Xike Xu, Jinxin Wang, Saisai Tian, Bin Xie, Yunheng Shen, Xianpeng Zu
Summary: The processing of Cistanche tubulosa enhances its efficacy in treating kidney-yang deficiency syndrome. Metabolite analysis revealed 47 differential metabolites in urine, with pathways such as purine metabolism, alanine, aspartate, and glutamate metabolism, and citrate cycle being the main targets. Additionally, 53 prototypes and 48 metabolites were detected in rats, providing valuable insights into the enhanced efficiency of processed Cistanche tubulosa and offering a strategy for analyzing chemical components and metabolites of other traditional Chinese medicine prescriptions.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Chemistry, Analytical
Li-Hong Ye, Juan Yang, Fang-Ming Liu, Jun Cao
Summary: A method for identification and difference analysis of active compounds in different species of hawthorn using ultrahigh-performance liquid chromatography coupled with mass spectrometry was proposed. The study investigated and optimized various parameters for separation and response, and successfully distinguished different species of hawthorn using mass spectrometry data combined with multivariate analysis.
MICROCHEMICAL JOURNAL
(2021)