Article
Multidisciplinary Sciences
Hao Ye, Jiayang Gao, Zizhen Liang, Youshun Lin, Qianyi Yu, Shuxian Huang, Liwen Jiang
Summary: This study identifies and demonstrates the role of Arabidopsis oxysterol-binding protein-related protein 2A (ORP2A) in mediating ER-autophagosomal membrane contacts and autophagosome biogenesis. ORP2A interacts with VAMP-associated protein 27-1 and ATG8e to form membrane contact sites between the ER and autophagosomes. Knockdown of ORP2A leads to impaired autophagy levels and inhibited plant growth. Additionally, ORP2A binds multiple phospholipids and colocalizes with phosphatidylinositol 3-phosphate (PI3P).
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Asami Kawasaki, Akiko Sakai, Hiroki Nakanishi, Junya Hasegawa, Tomohiko Taguchi, Junko Sasaki, Hiroyuki Arai, Takehiko Sasaki, Michihiro Igarashi, Fubito Nakatsu
Summary: This study reveals that ORP10 mediates lipid countertransport at ER-endosome MCSs, supplying endosomes with phosphatidylserine (PS) in exchange for phosphatidylinositol 4-phosphate (PI4P), thereby promoting EHD1 recruitment and endosome fission.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Javier Encinar del Dedo, Isabel Maria Fernandez-Golbano, Laura Pastor, Paula Meler, Cristina Ferrer-Orta, Elena Rebollo, Maria Isabel Geli
Summary: The study reveals the formation of ERSESs (ER sterol exit sites) within cellular membranes, where the sterol transporter Osh2 extracts sterols. Coupling of the sterol synthesis and transport machineries is essential for endocytosis in mother cells, but not in daughters.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrea Eisenreichova, Bartosz Rozycki, Evzen Boura, Jana Humpolickova
Summary: Osh6, a member of the ORP family, is a lipid transport protein responsible for transporting phosphatidylserine (PS) between the endoplasmic reticulum (ER) and the plasma membrane (PM). While PI4P and PS are the most commonly transported cargo molecules, it was also discovered that Osh6 can transport PIP2.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Cell Biology
Yi Xin (Iris) Tu, Andrew M. Sydor, Etienne Coyaud, Estelle M. N. Laurent, Diana Dyer, Nora Mellouk, Jonathan St-Germain, Robert M. Vernon, Julie D. Forman-Kay, Taoyingnan Li, Rong Hua, Kexin Zhao, Neale D. Ridgway, Peter K. Kim, Brian Raught, John H. Brumell
Summary: This study identified a network of interactions among core human macroautophagy proteins using proximity-dependent biotin identification, highlighting the critical role of OSBPL family members in the macroautophagy process.
Article
Biology
Nina Criado Santos, Samuel Bouvet, Maria Cruz Cobo, Marion Mandavit, Flavien Bermont, Cyril Castelbou, Farah Mansour, Maral Azam, Francesca Giordano, Paula Nunes-Hasler
Summary: Sec22b regulates phagosome maturation by tethering ER-phagosome membrane contact sites independently of the tether STIM1. It affects calcium signaling, phagolysosome fusion, antigen degradation and phagosomal phospholipids. Sec22b co-precipitates with the PS/PI(4)P exchange protein ORP8 and restores phagosomal PI(4)P levels.
COMMUNICATIONS BIOLOGY
(2023)
Editorial Material
Cell Biology
Rodrigo Enrique Gomez, Julie Castets, Josselin Lupette, Clement Chambaud, Jerome Joubes, Amelie Bernard
Summary: Autophagy is a key mechanism in plants that helps them adapt to various environmental constraints. The molecular mechanisms, especially autophagosome formation, remain poorly understood. A recent study used biochemistry, genetics, cell biology, and high-resolution 3D imaging to uncover the function of lipid phosphatidylinositol-4-phosphate (PtdIns4P) in autophagy in Arabidopsis thaliana, providing new insights into autophagosome assembly in plant cells.
Article
Cell Biology
Melody Subra, Bruno Antonny, Bruno Mesmin
Summary: VAP-A is a crucial ER receptor facilitating membrane contact sites between organelles. One well-studied example is its interaction with OSBP, facilitating the transfer of cholesterol from ER to the trans-Golgi network. This review highlights recent studies that enhance our understanding of the OSBP cycle and broaden the model of lipid exchange in different cellular contexts and conditions.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Cell Biology
Minami Orii, Takuma Tsuji, Yuta Ogasawara, Toyoshi Fujimoto
Summary: The study reveals that Atg9-mediated phospholipid transport plays a crucial role in the expansion of autophagosomes. Analyses of various phospholipids distribution in autophagosomes suggest that transbilayer phospholipid movement mediated by Atg9 is involved in the biogenesis of autophagosomes.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Wenbin Zhong, Weize Lin, Yingjie Yang, Dan Chen, Xiuye Cao, Mengyang Xu, Guoping Pan, Huanzhao Chen, Jie Zheng, Xiaoqin Feng, Li Hua Yang, Chaofeng Lai, Vesa M. Olkkonen, Jun Xu, Shuzhong Cui, Daoguang Yan
Summary: This study reveals the role of ORP4L in T-cell leukemogenesis by orchestrating the transport of PI(4)P, contributing to PI3K/AKT hyperactivation.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Microbiology
James B. Konopka
Summary: Phosphatidylinositol 4-phosphate (PI4P) plays novel roles in the plasma membrane of the fungal pathogen Candida albicans, including hyphal growth and cell wall organization. Deleting the STT4 kinase selectively eliminates the plasma membrane pool of PI4P, leading to defective disseminated candidiasis in mice but not oral infection.
Article
Cell Biology
Lindsay A. Heckle, Keith G. Kozminski
Summary: Polarized secretion is important for polarized cell growth. A protein called oxysterol-binding protein homologue 4 (Osh4p) is thought to establish a gradient of a lipid called phosphatidylinositol 4-phosphate (PI4P) along the secretory pathway, which helps vesicles dock at the plasma membrane. However, this study found that the levels of PI4P remain constant as vesicles traffic from the trans-Golgi to the plasma membrane. The researchers propose a new model where other factors, including Rab GTPases, play a role in vesicle maturation.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Biochemistry & Molecular Biology
Shinya Mochizuki, Harukata Miki, Ruyun Zhou, Yasuko Noda
Summary: This study reveals the role of ORP6 in the counter-transport of PI4P and PS. By analyzing the localization shift of specific markers, researchers confirm the involvement of ORP6 in the exchange of PI4P and PS on the cell membrane.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Plant Sciences
Gwennogan A. Dubois, Yvon Jaillais
Summary: Anionic phospholipids, such as phosphatidic acid and phosphatidylserine, are low-abundant lipids that play important roles in membrane functions. They accumulate in different endomembranes according to unique subcellular patterns and form concentration gradients across organelles, challenging the initial belief in their compartment-specificity.
Article
Plant Sciences
Ryo Kuroda, Mariko Kato, Tomohiko Tsuge, Takashi Aoyama
Summary: This study investigated the biological functions of the PIP5K7-9 subgroup genes in Arabidopsis thaliana, revealing their preferential expression in specific tissues and plasma membrane localization in root meristem cells. The genes were found to be redundant in adapting root growth to hyperosmotic conditions, possibly through promoting plasma membrane recycling in root meristem cells.
Article
Biochemistry & Molecular Biology
Asako Goto, Mark Charman, Neale D. Ridgway
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Cell Biology
Mark Charman, Asako Goto, Neale D. Ridgway
Article
Cell Biology
Kexin Zhao, Neale D. Ridgway
Article
Biochemistry & Molecular Biology
Parthajit Mukherjee, Hasam Madarati, Neale D. Ridgway, Jeffrey Atkinson
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Article
Biochemistry & Molecular Biology
Kexin Zhao, Aarnoud van der Spoel, Claudia Castiglioni, Sarah Gale, Hideji Fujiwara, Daniel S. Ory, Neale D. Ridgway
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2018)
Review
Biochemistry & Molecular Biology
Antonietta Pietrangelo, Neale D. Ridgway
CELLULAR AND MOLECULAR LIFE SCIENCES
(2018)
Review
Biochemistry & Molecular Biology
Neale D. Ridgway, Kexin Zhao
CURRENT OPINION IN LIPIDOLOGY
(2018)
Article
Cell Biology
Jin Huang, Carl J. Mousley, Louis Dacquay, Nairita Maitra, Guillaume Drin, Chong He, Neale D. Ridgway, Ashutosh Tripathi, Michael Kennedy, Brian K. Kennedy, Wenshe Liu, Kristin Baetz, Michael Polymenis, Vytas A. Bankaitis
DEVELOPMENTAL CELL
(2018)
Editorial Material
Biochemistry & Molecular Biology
Neale D. Ridgway
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Jonghwa Lee, Neale D. Ridgway
JOURNAL OF LIPID RESEARCH
(2018)
Article
Cell Biology
Asako Goto, Mark Charman, Neale D. Ridgway
Article
Multidisciplinary Sciences
Antonietta Pietrangelo, Neale D. Ridgway
Review
Biochemistry & Molecular Biology
Wasitha P. D. Wass Thilakarathna, H. P. Vasantha Rupasinghe, Neale D. Ridgway
Summary: HCC is primarily caused by chronic infections such as hepatitis B and C viruses, alcoholic liver disease, and non-alcoholic fatty liver disease. Genetic background and single nucleotide polymorphisms also play a role in HCC development. Probiotics can mitigate HCC risk by modulating gut microbiota, reducing inflammation, and exhibiting antiviral and anticancer effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gabriel G. Dorighello, Leandro H. P. Assis, Thiago Rentz, Joseane Morari, Monique F. M. Santana, Marisa Passarelli, Neale D. Ridgway, Anibal E. Vercesi, Helena C. F. Oliveira
Summary: The expression of CETP in macrophages modulates mitochondrial structure and function, promotes an intracellular antioxidant state and oxidative metabolism, and reduces the expression of inflammatory genes and cholesterol accumulation.