Article
Biophysics
Konstantin Speckner, Lorenz Stadler, Matthias Weiss
Summary: This study reveals the complex structure of the ER in mammalian cells and its impact on the distribution of ERESs, suggesting that the spatial pattern of ERESs is determined by ER morphology and maintained by hindering the process of domain coarsening. This mechanism ensures a proper functioning of ERES patterns in the early secretory pathway under various conditions.
BIOPHYSICAL JOURNAL
(2021)
Article
Cell Biology
Swathi Devireddy, Shawn M. Ferguson
Summary: This study reveals the mechanism of efficient delivery of Progranulin and prosaposin to lysosomes, where the interaction between prosaposin and Surf4 plays a key role in the process, highlighting its relevance to neurodegenerative diseases.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Xiao Wang, Runze Huang, Yawei Wang, Wenjing Zhou, Yating Hu, Yuanhang Yao, Kunlun Cheng, Xin Li, Bolin Xu, Jie Zhang, Yaowen Xu, Fanxin Zeng, Yuangang Zhu, Xiao-Wei Chen
Summary: Wang et al. discovered that the COPII machinery uses manganese-tuning, self-constrained condensation to selectively drive lipoprotein delivery and set lipid homeostasis. Manganese binds to the inner COPII coat and enhances condensation, shifting the balance of the transport machinery. Manganese can be mobilized from mitochondria to signal COPII and selectively control lipoprotein secretion with a distinctive function. This study reveals the importance of dietary manganese in the treatment of cardiovascular metabolic health.
NATURE CELL BIOLOGY
(2023)
Article
Biology
Keiko Saegusa, Kohichi Matsunaga, Miharu Maeda, Kota Saito, Tetsuro Izumi, Ken Sato
Summary: Surf4 regulates the ER export of proinsulin through its recruitment to ERES and interacts physically with proinsulin, Sec12, and COPII.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dalal Bakhos-Douaihy, Elie Seaayfan, Nadia Frachon, Sylvie Demaretz, Martin Koemhoff, Kamel Laghmani
Summary: This study reveals that NKCC2 relies on a COPII-dependent pathway for ER export, in addition to its dileucine-like motifs, it also requires di-acidic motifs. The findings suggest that mutations interfering with this pathway could be the molecular basis of BS1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mingqin Chang, Shu-Zon Wu, Samantha E. Ryken, Jacquelyn E. O'Sullivan, Magdalena Bezanilla
Summary: Plant COPII complex in moss Physcomitrium patens displays isoform-specific contributions to polarized growth, with different Sec23/24 gene families playing distinct roles in development. The presence of divergent Sec23 clades in land plants suggests functional diversification within the COPII complex.
Review
Cell Biology
Sharanya Chatterjee, Ana Jeemin Choi, Gad Frankel
Summary: The trafficking of cargo from the endoplasmic reticulum (ER) to the Golgi apparatus is a crucial cellular process involving the coat protein complex-II (COPII) and Sec24. Approximately one-third of mammalian proteins require the COPII-mediated secretory pathway for membrane insertion or secretion. There are distinct cargo interaction motifs for different Sec24 paralogs, leading to varying levels of specificity in cargo binding. Additionally, the Sec24 secretion system can be exploited by pathogens like viruses and bacteria.
Article
Biochemistry & Molecular Biology
Xuehong Qian, Lingang He, Jiejie Yang, Jiajia Sun, Xueying Peng, Yuting Zhang, Yizhou Mao, Ying Zhang, Yixian Cui
Summary: In this study, we discovered that the autophagy regulator UVRAG plays a crucial role in orchestrating the assembly of ER-phagy sites (ERPHS). UVRAG localizes to ERPHs and interacts with specific ER-phagy cargo receptors, regulating their oligomerization and facilitating the recruitment of core autophagy proteins.
Review
Biochemistry & Molecular Biology
Ishier Raote, Sonashree Saxena, Felix Campelo, Vivek Malhotra
Summary: TANGO1 protein assists in the export of large cargo from the endoplasmic reticulum (ER) by creating a specific domain and assembling into a ring on the ER membrane plane, allowing cargo transfer without the need for vesicular intermediates. Its evolution and activities differentiate between yeast and animal early secretory pathways.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Review
Cell Biology
Pierre Bensidoun, Daniel Zenklusen, Marlene Oeffinger
Summary: The nuclear pore complex (NPC) is the central gate for mRNA transportation from the nucleus to the cytoplasm. The export competence of mRNAs is linked to upstream nuclear processes, and recent evidence suggests that the NPC may be more heterogeneous than previously believed, providing functional plasticity to mRNA export. Interconnected processes of nuclear mRNA metabolism and NPC heterogeneity play crucial roles in regulating export competence.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2021)
Article
Cell Biology
Olga Shomron, Inbar Nevo-Yassaf, Tamar Aviad, Yakey Yaffe, Eitan Erez Zahavi, Anna Dukhovny, Eran Perlson, Ilya Brodsky, Adva Yeheskel, Metsada Pasmanik-Chor, Anna Mironov, Galina Beznoussenko, Alexander A. Mironov, Ella H. Sklan, George H. Patterson, Yoji Yonemura, Mara Sannai, Christoph Kaether, Koret Hirschberg
Summary: The study reveals a novel mode of function for COPII in cargo export from the ER by facilitating selective cargo concentration at the boundary between ER and ER exit sites. COPII plays a crucial role in cargo selection and concentration, rather than simply coating Golgi-bound carriers.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Genetics & Heredity
Xenia Georgiou, Sofia Dimou, George Diallinas, Martina Samiotaki
Summary: This study aims to investigate the mechanism of membrane trafficking and Golgi bypass. By using PDB and mass spectrometry analysis, transient interactors of UapA transporter were identified, and the importance of COPI coatomer and ArfA GTPase activating protein Glo3 in UapA trafficking was validated.
FUNGAL GENETICS AND BIOLOGY
(2023)
Review
Medicine, Research & Experimental
Vi T. Tang, David Ginsburg
Summary: Most proteins destined for different compartments within and outside the cell need to pass through the intracellular secretory pathway. This process involves the recruitment and transport of cargoes from the endoplasmic reticulum (ER) to the Golgi apparatus by coat protein complex II (COPII) and cargo receptors. Gene duplication events have led to multiple COPII paralogs in mammals. This review discusses the functions of each COPII protein, their associations with human disorders, and the conservation of their functions among paralogs. Additionally, it summarizes the roles of two prototypical cargo receptors, LMAN1 and SURF4, in human health and disease.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Cell Biology
Raffaella Gallo, Arpan Kumar Rai, Alexa B. R. Mcintyre, Katrina Meyer, Lucas Pelkmans
Summary: The dual-specificity kinase DYRK3 plays an important role in regulating the formation and dissolution of biomolecular condensates, affecting processes such as stress recovery and mitotic progression. This study shows that DYRK3 interacts with proteins associated with endoplasmic reticulum exit sites (ERESs), and its inhibition disrupts the organization of the ERES-Golgi interface and secretory trafficking. The regulation of ERES by DYRK3 depends on the N-terminal intrinsically disordered region (IDR) of the peripheral membrane protein SEC16A, which forms liquid-like condensates with ERES components. The findings suggest that the physical state of ERES is crucial for efficient membrane traffic in eukaryotic cells, and DYRK3 phosphorylation plays a role in modulating these material properties.
DEVELOPMENTAL CELL
(2023)
Article
Cell Biology
Rosanne Wouters, Christine Michiels, Ragna Sannerud, Bertrand Kleizen, Katleen Dillen, Wendy Vermeire, Abril Escamilla Ayala, David Demedts, Randy Schekman, Wim Annaert
Summary: gamma-secretase plays a role in various physiological processes by targeting over 100 substrates, with dysfunction linking it to cancer and Alzheimer's disease. The spatiotemporal regulation of its assembly remains unclear, but evidence suggests a stepwise process controlled by selective sorting mechanisms, leading to final levels in postGolgi compartments.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dariusz Zakrzewicz, Miroslava Didiasova, Marcus Krueger, Benedetto Daniele Giaimo, Tilman Borggrefe, Maren Mieth, Andreas C. Hocke, Anna Zakrzewicz, Liliana Schaefer, Klaus T. Preissner, Malgorzata Wygrecka
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2018)
Article
Gastroenterology & Hepatology
Anke Hermann, Dirk Oliver Wennmann, Sascha Gromnitza, Maria Edeling, Veerle Van Marck, Marius Sudol, Liliana Schaefer, Kerstin Duning, Thomas Weide, Hermann Pavenstaedt, Joachim Kremerskothen
Article
Biochemistry & Molecular Biology
Heiko Roedig, Madalina V. Nastase, Helena Frey, Kristin Moreth, Jinyang Zeng-Brouwers, Chiara Poluzzi, Louise Tzung-Harn Hsieh, Christian Brandts, Simone Fulda, Malgorzata Wygrecka, Liliana Schaefer
Article
Biochemistry & Molecular Biology
Lukasz Wujak, Jennifer Schnieder, Liliana Schaefer, Malgorzata Wygrecka
Review
Biochemistry & Molecular Biology
Heiko Roedig, Madalina Viviana Nastase, Malgorzata Wygrecka, Liliana Schaefer
Article
Urology & Nephrology
Chiara Poluzzi, Madalina-Viviana Nastase, Jinyang Zeng-Brouwers, Heiko Roedig, Louise Tzung-Harn Hsieh, Jonas B. Michaelis, Eva Miriam Buhl, Flavia Rezende, Yosif Manavski, Andre Prime Bleich, Peter Boor, Ralf P. Brandes, Josef Pfeilschifter, Ernst H. K. Stelzer, Christian Muench, Ivan Dikic, Christian Brandts, Renato V. Iozzo, Malgorzata Wygrecka, Liliana Schaefer
KIDNEY INTERNATIONAL
(2019)
Editorial Material
Cell Biology
Liliana Schaefer
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Liliana Schaefer, Ivan Dikic
Summary: Recent research has shown the crucial regulatory roles of the extracellular matrix (ECM) in autophagy, with various ECM-derived proteoglycans and proteins inducing or suppressing autophagy. Developing ECM-derived therapeutics selectively regulating autophagy holds vast pharmacological potential.
Article
Biochemistry & Molecular Biology
Martin Schulz, Valentina Diehl, Jonel Trebicka, Malgorzata Wygrecka, Liliana Schaefer
Summary: Soluble biglycan plays a crucial role in inflammation and autophagy, showing diverse functions in inflammatory kidney diseases and chronic liver diseases. Hepatorenal dysfunction is a common complication in patients with cirrhosis, where soluble biglycan may play a key role.
Editorial Material
Cell Biology
Liliana Schaefer
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Timon Eckes, Sammy Patyna, Alexander Koch, Anke Oftring, Stefan Gauer, Nicholas Obermueller, Stephanie Schwalm, Liliana Schaefer, Jerold Chun, Hermann-Josef Groene, Josef Pfeilschifter
Summary: This study found that S1P(5) deficiency can improve tubular damage and tubulointerstitial fibrosis in a mouse model of adenine-induced nephropathy, and reduce inflammation. Therefore, targeting S1P(5) may be a promising therapeutic target for kidney diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Fabian Schramm, Liliana Schaefer, Malgorzata Wygrecka
Summary: This review article provides an overview of the ErbB receptor-ligand system and its importance in developmental and physiological processes. It focuses on the role of ErbB receptors and their ligands in maladaptive remodeling of lung tissue, specifically in idiopathic pulmonary fibrosis (IPF). The article discusses the pathways and cellular processes contributing to miscommunication between epithelial and mesenchymal cells in IPF, and reviews in vivo studies on the efficacy of ErbB signaling inhibitors in lung injury models. Additionally, it discusses lessons learned from clinical applications of ErbB1 signaling inhibitors in cancer to inform clinical trials in IPF.
Article
Biology
Keiko Saegusa, Kohichi Matsunaga, Miharu Maeda, Kota Saito, Tetsuro Izumi, Ken Sato
Summary: Surf4 regulates the ER export of proinsulin through its recruitment to ERES and interacts physically with proinsulin, Sec12, and COPII.
COMMUNICATIONS BIOLOGY
(2022)