Article
Biochemistry & Molecular Biology
Yuanyuan Liu, Panpan Zhu, Sen Cai, George Haughn, Jonathan E. Page
Summary: Three novel transcription factors were found to interact with the THCAS promoter in cannabis, with CsAP2L1 having a positive regulatory role and CsWRKY1 and CsMYB1 possibly acting as negative regulators. This study provides insights into the molecular mechanisms underlying the transcriptional control of THCAS synthesis in cannabis.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Agricultural Engineering
Sanush K. Abeysekera, Amanda Robinson, Melanie Po-Leen Ooi, Ye Chow Kuang, Merilyn Manley-Harris, Wayne Holmes, Evan Hirst, Jessika Nowak, Manu Caddie, Gregor Steinhorn, Serge Demidenko
Summary: This paper presents a reliable real-time technique using proximal near infrared hyperspectral imaging to measure the concentration of tetrahydrocannabinolic acid (THCA) in hemp. The study compares different regression algorithms and finds that regularized partial least squares (RPLS) achieves the best performance. A variation of RPLS with feature selection (PLSFS) is introduced to improve model interpretability and reproducibility.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Andrey Valentinovich Zadorozhny, Mikhail Evgenyevich Voskoboev, Denis Vladimirovich Bochkov, Alexei Sergeyevich Rozanov, Elizaveta Dmitrievna Shedko, Irina Anatolyevna Mescheryakova, Alexander Gennadyevich Blinov, Anton Vladimirovich Korzhuk, Valeria Nikolayevna Shlyakhtun, Natalia Vladimirovna Bogacheva, Egor Vladimirovich Antonov, Svetlana Valerevna Bannikova, Tatiana Nikolayevna Goryachkovskaya, Sergey Evgenyevich Peltek
Summary: The neutral protease pAsPs gene from Aspergillus pseudotamarii was successfully obtained and integrated into the genome of yeast strain Komagataella phaffii T07. The recombinant enzyme showed high specific activity towards casein and was inhibited by Cu2+. Mass-spectrometric analysis confirmed the consistency of the obtained pAsPs preparation with the predicted amino acid sequence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Legal
Madeline G. Roman, Ya-Chih Cheng, Sarah Kerrigan, Rachel Houston
Summary: Marijuana is classified as an illicit drug in the Controlled Substances Act, but the recent U.S. Farm Bill removed it from the definition of marijuana, making it a legal crop. This study optimized and evaluated a DNA analysis method for determining crop type and reported the presence or absence of 15 cannabinoids in cannabis reference materials and hemp flower samples. Factors other than the genetic variation of the THCA synthase gene should be considered when determining crop type.
JOURNAL OF FORENSIC SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Ziwei Zhou, Hualan Zhou, Jianguo Zhang
Summary: The optimal detoxification process of wheat bran hydrolysate (WBH) was established in this research for the expression of heterologous protein by Komagataella phaffii, resulting in a low-cost medium alternative and a promising outlet for wheat bran utilization.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Aibo Feng, Jingyao Zhou, Hongli Mao, Hualan Zhou, Jianguo Zhang
Summary: Using formate as an inducer instead of methanol can increase the expression level of heterologous proteins in K. phaffii, providing new possibilities for high-level production of heterologous proteins.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Plant Sciences
Jana Murovec, Jan Jurij Erzen, Marko Flajsman, Dominik Vodnik
Summary: This study investigated the correlation between visual appearance and chemotype of medical cannabis plants. The results showed that morphological differences did not accurately predict the cannabinoid profiles. However, the THCAS gene sequence analysis was able to discriminate between different chemotypes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Crist N. Filer
Summary: There is a minor structural isomer called THCA-B in Cannabis, and currently no reasonable biochemical pathway has been found to explain its presence. It is generally believed that THCA-A is the exclusive product of THCA synthase. Therefore, a synthetic route proposing a photochemical mechanism has been suggested to convert THCA-A to CBDA, and then convert CBDA to THCA-B.
CANNABIS AND CANNABINOID RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Hualan Zhou, Feng Wang, Hongli Mao, Xuedong Wang, Jianguo Zhang
Summary: In this study, a genetically modified Komagataella phaffii strain was constructed to enable heterologous protein expression without the use of toxic and flammable methanol inducer. It was found that the use of a bioelectrical reactor and formate as a safe inducer resulted in efficient heterologous protein expression. This study successfully developed a safe recombinant K. phaffii system for heterologous protein expression and demonstrated a new approach for the utilization of CO2.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Jefree J. Raslan-Jaramillo, Gisela A. Rios-Gajardo, Marcia A. Avello, Marta G. de Diego
Summary: This study developed and validated a simple and rapid HPLC method for the determination of CBD and THC in cannabis beverages and ice cream. The method demonstrated good stability and can be useful for quality control purposes.
JOURNAL OF AOAC INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Francesco Tolomeo, Fabiana Russo, Dominika Kaczorova, Maria Angela Vandelli, Giuseppe Biagini, Aldo Lagana, Anna Laura Capriotti, Roberta Paris, Flavia Fulvio, Luigi Carbone, Elisabetta Perrone, Giuseppe Gigli, Giuseppe Cannazza, Cinzia Citti
Summary: This article discusses the major phytocannabinoids in Cannabis sativa, their derivatives, and the recent discovery of a new cannabinoid. The quantification of these compounds in different cannabis samples and their impact on classification are also explored.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Alessandro Monari, Sara Cantalu, Barbara Zanfrognini, Virginia Brighenti, Patrizia Verri, Chiara Zanardi, Federica Pellati, Laura Pigani
Summary: Two electrochemical sensors were developed for rapid screening of cannabinoids in Cannabis sativa L. plant material. Control of cannabinoid content is important for distinguishing between recreational and fibre-type C. sativa samples, which differ in their THCA and THC levels.
Article
Medicine, Legal
Tadashi Yamamuro, Hiroki Segawa, Kenji Kuwayama, Kenji Tsujikawa, Tatsuyuki Kanamori, Yuko T. Iwata
Summary: This study aimed to distinguish between drug-type and fiber-type cannabis using a PCR-based method targeting the differences in THCAS and CBDAS gene sequences. It was found that a mixed primer set in an allele-specific duplex PCR system successfully suppressed unintended nonspecific amplification and differentiated between the two types of cannabis. This identification method could be useful for various purposes, such as detecting genetic contamination in industrial hemp and forensic examinations related to cannabis.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Medicine, Legal
Otyllia R. Abraham, Ruth Waddell Smith
Summary: In this study, optical and spectroscopic techniques were used to characterize solvent extracts derived from marijuana and hemp, revealing differences in crystal structure and composition between the two samples. Polarized light microscopy and infrared spectroscopy were used to distinguish crystals and differentiate structural differences, while single-crystal X-ray diffraction confirmed the presence of tetrahydrocannabinolic acid in marijuana-derived extracts and cannabidiol in hemp-derived extracts. Optical characterization provides a screening method to visually differentiate similar samples before confirmatory analysis.
JOURNAL OF FORENSIC SCIENCES
(2022)
Article
Plant Sciences
Kelly F. Boddington, Eric Soubeyrand, Kristen Van Gelder, Jose A. Casaretto, Colby Perrin, Taylor J. B. Forrester, Cameron Parry, M. Sameer Al-Abdul-Wahid, Nicholas G. Jentsch, Jakob Magolan, Gale G. Bozzo, Matthew S. Kimber, Steven J. Rothstein, Tariq A. Akhtar
Summary: This study identifies a set of enzymes in Cannabis involved in the biosynthesis of bibenzyls, contributing valuable insights for the rational metabolic engineering of natural product therapeutics.
Article
Biotechnology & Applied Microbiology
Bastian Zirpel, Friederike Degenhardt, Chantale Martin, Oliver Kayser, Felix Stehle
JOURNAL OF BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Marcus Geissler, Jascha Volk, Felix Stehle, Oliver Kayser, Heribert Warzecha
BIOTECHNOLOGY LETTERS
(2018)
Article
Plant Sciences
Julia Schachtsiek, Heribert Warzecha, Oliver Kayser, Felix Stehle
Article
Biotechnology & Applied Microbiology
Bastian Zirpel, Oliver Kayser, Felix Stehle
JOURNAL OF BIOTECHNOLOGY
(2018)
Article
Plant Sciences
Paul Ebersbach, Felix Stehle, Oliver Kayser, Erik Freier
Letter
Biotechnology & Applied Microbiology
Julia Schachtsiek, Felix Stehle
PLANT BIOTECHNOLOGY JOURNAL
(2019)
Review
Plant Sciences
Tajammul Hussain, Richard V. Espley, Jurg Gertsch, Tracey Whare, Felix Stehle, Oliver Kayser
PHYTOCHEMISTRY REVIEWS
(2019)
Article
Biochemical Research Methods
Julia Schachtsiek, Tajammul Hussain, Khadija Azzouhri, Oliver Kayser, Felix Stehle
Article
Chemistry, Multidisciplinary
Hannes Meinert, Dong Yi, Bastian Zirpel, Eva Schuiten, Torsten Geissler, Egon Gross, Stephan Brueckner, Beate Hartmann, Carsten Roettger, Jakob P. Ley, Uwe T. Bornscheuer
Summary: The discovery of 66 novel bacterial CHIs reveals diversity in substrate specificity towards various hydroxylated and methoxylated chalcones. Mutagenesis of CHIera based on the binding models of these novel bacterial CHIs resulted in variants with significantly improved activity. Preparative scale conversion catalyzed by bacterial CHIs showed (S)-selectivity with up to 96% ee, providing an alternative biocatalytic route for the synthesis of (S)-flavanones in high yields.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Julia Schachtsiek, Felix Stehle
Review
Biotechnology & Applied Microbiology
Angela Carvalho, Esben Halkaejr Hansen, Oliver Kayser, Simon Carlsen, Felix Stehle
FEMS YEAST RESEARCH
(2017)