期刊
JOURNAL OF BIOTECHNOLOGY
卷 282, 期 -, 页码 18-24出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2018.06.340
关键词
Bioactive plants; Antioxidants; Antimicrobial; Antifungal; Sterols; Dehydroabietic acid
资金
- Slovak Scientific Grant Agency [VEGA-1/0353/16]
- Slovak Research and Development Agency [APVV-11-0850, APVV-14-0393, APVV-15-0052, APVV-16-0088]
- grant for young scientists at STU [1675]
- Operation Program Research and Development for the project National centre for research and application of renewable energy sources - European Regional Development Fund [ITMS 26240120028]
- Operation Program Research and Development for the project Competence centre for new materials, advanced technologies and energy - European Regional Development Fund [ITMS 26240220073]
- Operation Program Research and Development for the project University science park STU Bratislava - European Regional Development Fund [ITMS 26240220084]
The current study focuses on the analysis of in vitro biological activity of extract from bark of Norway spruce (Picea Abies), which can find potential application in food and cosmetic industry and pharmacology. Milled bark was subjected to Soxhlet extraction and supercritical fluid extraction to obtain two ethanol extracts. These extracts were further used to obtain their pre-extracts to n-hexane. It was investigated whether beta-sitosterol exhibits bacteriostatic activity necessary to observe antimicrobial and antifungal activity of methyl dehydroabiatate. This synergic effect and bacteriostatic activity of beta-sitosterol have not been previously reported. The greatest inhibition zone of n-hexane pre-extracts was confirmed in bacterium Pseudomonas aeruginosa (0,9 - 1,5 cm) and yeast Alternaria alternata (0,7 - 1,6 cm). It is novel, the antioxidant, antimicrobial and antifungal activity of spruce bark extracts assessed in terms of food and cosmetic fortification.
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