期刊
JOURNAL OF BIOPHOTONICS
卷 11, 期 10, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201800009
关键词
biosensor; diatom; fluorescence imaging; fluorescence spectroscopy; immunoassay; photonic crystal enhanced fluorescence
资金
- Directorate for Engineering [1701329]
- National Institute of Biomedical Imaging and Bioengineering [R03EB018893]
- National Institute of Food and Agriculture [3017-67021-26606]
- National Institute on Drug Abuse [1R21DA0437131]
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R03EB018893] Funding Source: NIH RePORTER
Fluorescence biosensing is one of the most established biosensing methods, particularly fluorescence spectroscopy and microscopy. These are two highly sensitive techniques but require high-grade electronics and optics to achieve the desired sensitivity. Efforts have been made to implement these methods using consumer grade electronics and simple optical setups for applications such as point-of-care diagnostics, but the sensitivity inherently suffers. Sensing substrates, capable of enhancing fluorescence are thus needed to achieve high sensitivity for such applications. In this paper, we demonstrate a photonic crystal-enhanced fluorescence immunoassay biosensor using diatom biosilica, which consists of silica frustules with sub-100 nm periodic pores. Utilizing the enhanced local optical field, the Purcell effect and increased surface area from the diatom photonic crystals, we create ultrasensitive immunoassay biosensors that can significantly enhance fluorescence spectroscopy as well as fluorescence imaging. Using standard antibody-antigen-labeled antibody immunoassay protocol, we experimentally achieved 100x and 10x better detection limit with fluorescence spectroscopy and fluorescence imaging respectively. The limit of detection of the mouse IgG goes down to 10(-16) M (14 fg/mL) and 10(-15) M (140 fg/mL) for the two respective detection modalities, virtually sensing a single mouse IgG molecule on each diatom frustule. The effectively enhanced fluorescence imaging in conjunction with the simple hot-spot counting analysis method used in this paper proves the great potential of diatom fluorescence immunoassay for point-of-care biosensing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据