期刊
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
卷 14, 期 7, 页码 1287-1297出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2018.2575
关键词
Hydrophobically Modified Chitosan; Gene Carrier; Ultrasonic Gene Transfer; Acoustic Droplet Vaporization; Cavitation
资金
- National Natural Science Foundation of China [81371572, 81671707, 11534013, 81527901]
- Natural Science Foundation of Guangdong Province [2016A030311054, 2014B030301013, 2017A030313651]
- Research Projects of Guangzhou Technology Bureau [201607010201]
- Shenzhen Science and Technology Innovation Committee [JCYJ20150521144321010, JCYJ20170413100222613, JCYJ20150521094519482]
- Research Fund for Lin He's Academician Workstation of New Medicine and Clinical Translation
- Scientific Research Innovation Fund of Guangzhou Medical University [2016A13, 2015A07]
To attain attractive ultrasound-responsive gene delivery, a new kind of echogenic chitosan nanodroplets (CND) was developed to explore the potential to deliver genes in a spatiotemporally controlled manner. Self-assembled amphiphilic chitosan micelles of nanoscale size were fabricated to encapsulate hydrophobic perfluoropentane into the inner cores. The resulting CND presented a positive surface charge. enabling the formation of nano-complexes with genetic cargo through electrostatic interactions. Agarose-gel electrophoresis further confirmed the ability of CND to bind DNA. CND was also observed to protect DNA from degradation by nucleases. A temperature-dependent droplet-to-bubble conversion was also demonstrated. More importantly, our study revealed that CND in combination with ultrasound could significantly enhance gene delivery. In conclusion, our study demonstrated a novel carrier with great potential for efficient ultrasound-mediated gene delivery to specific tissues in a spatiotemporally controlled manner.
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