期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 29, 期 13, 页码 1625-1642出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2018.1479084
关键词
Interfacial polyelectrolyte complexation; neuron; neurite; hydrogel; electrospinning
资金
- VA Rehab RD [I21-RX001904]
- National Institute of Dental and Craniofacial Research at NIH [2 T32 DE007057-40]
- Department of Defense [W91ZSQ2136N601]
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [T32DE007057] Funding Source: NIH RePORTER
- Veterans Affairs [I21RX001904] Funding Source: NIH RePORTER
A promising component of biomaterial constructs for neural tissue engineering are electrospun fibers, which differentiate stem cells and neurons as well as direct neurite growth. However, means of protecting neurons, glia, and stem cells seeded on electrospun fibers between lab and surgical suite have yet to be developed. Here we report an effort to accomplish this using cell-encapsulating hydrogel fibers made by interfacial polyelectrolyte complexation (IPC). IPC-hydrogel fibers were created by interfacing acid-soluble chitosan (AsC) and cell-containing alginate and spinning them on bundles of aligned electrospun fibers. Primary spinal astrocytes, cortical neurons, or L929 fibroblasts were mixed into alginate hydrogels prior to IPC-fiber spinning. The viability of each cell type was assessed at 30min, 4h, 1d, and 7d after encapsulation in IPC hydrogels. Some neurons were encapsulated in IPC-hydrogel fibers made from water-soluble chitosan (WsC). Neurons were also stained with Tuj1 and assessed for neurite extension. Neuron survival in AsC-fibers was worse than astrocytes in AsC-fibers (p<0.05) and neurons in WsC-fibers (p<0.05). As expected, neuron and glia survival was worse than L929 fibroblasts (p<0.05). Neurons in IPC-hydrogel fibers fabricated with WsC extended neurites robustly, while none in AsC fibers did. Neurons remaining inside IPC-hydrogel fibers extended neurites inside them, while others de-encapsulated, extending neurites on electrospun fibers, which did not fully integrate with IPC-hydrogel fibers. This study demonstrates that primary neurons and astrocytes can be encapsulated in IPC-hydrogel fibers at good percentages of survival. IPC hydrogel technology may be a useful tool for encapsulating neural and other cells on electrospun fiber scaffolds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据