期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 29, 期 10, 页码 1207-1218出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2018.1448336
关键词
Chitosan oligosaccharide; osteoclast; p38; c-Jun N-terminal kinase; extracellular signal-regulated kinase; protein kinase B
资金
- Natural Science Foundation of Zhejiang Province [LY16H250002]
- National Natural Science Fund of China [81772348]
Chitosan Oligosaccharide (COS) has been widely used for the systemic treatment of clinical diseases such as bone tissue engineering. However, its influence on osteoclast formation, which plays a critical role in bone homeostasis, has never been investigated. The aim of this study was to investigate the effect of chitosan oligosaccharide on differentiation of osteoclast. Using cell counting kit-8, tartrate-resistant acid phosphatase staining, reverse transcription-quantitative polymerase chain reaction assay and western blot analysis, we demonstrated that chitosan oligosaccharide cannot inhibit RANKL-inducedosteoclast precursor proliferation but does promote osteoclast differentiation by stimulating the activation of p38/mitogen-activated protein kinase(MAPK), c-Jun N-terminal kinase (JNK)/MAPK, extracellular signal-regulated kinase (ERK)/MAPK and protein kinase B (AKT) without affecting nuclear factor kappaB (NF-kB)signaling pathways. Based on the promoting effect of chitosan oligosaccharide on osteoclast differentiation, we suggest that this property of chitosan oligosaccharide may have potential detrimental effect on bone homeostasis.
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