Article
Agriculture, Dairy & Animal Science
T. Larsen, R. Rupp, N. C. Friggens, J. A. A. Pires
Summary: This study presents a novel method for quantifying the activity of the enzyme isocitrate dehydrogenase (IDH) in ruminant milk without pretreatment of the sample. The results show that IDH activity significantly increases during a period of nutritional restriction, accompanied by an increase in the metabolite isocitrate concentration and somatic cell count, and returns to the initial level shortly after the restriction period.
Article
Cell Biology
Isabelle A. MacLean, Anchal Varma, Kenneth B. Storey
Summary: This study investigated the dynamic nature of NADP-IDH during hibernation, revealing increased substrate affinity and protein phosphorylation levels. These changes suggest the ability of the organism to reduce energy consumption and enhance antioxidant activity during hibernation.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ryan A. Herold, Christopher J. Schofield, Fraser A. Armstrong
Summary: The study utilizes a nanoporous electrode material, the e-Leaf, to control enzyme cascades and gain detailed kinetic insight into an anti-cancer drug mechanism. The e-Leaf allows for the quantification of IDH1 R132H inhibition kinetics and reveals factors underlying inhibitor residence time. The study highlights the importance of this method in obtaining detailed kinetic and mechanistic information that is difficult to obtain using conventional techniques.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Ryan A. Herold, Raphael Reinbold, Christopher J. Schofield, Fraser A. Armstrong
Summary: Electrochemical studies reveal that nanoconfinement significantly increases the efficiency of enzyme-catalyzed cascade reactions. By using a nanoporous conducting indium tin oxide film and entrapping Isocitrate dehydrogenase 1 (IDH1), the complete electrochemical oxidation of isocitrate to 2-oxoglutarate is achieved using only the NADP(H) cofactor carried into the electrode pores. The results demonstrate the power of nanoconfinement in facilitating multistep enzyme catalysis and provide insights into the role of nicotinamide cofactors as redox carriers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Jannik Zimmermann, Julian Oestreicher, Fabian Geissel, Marcel Deponte, Bruce Morgan
Summary: A novel high-throughput assay has been developed for rapid and semi-quantitative evaluation of glutathione-dependent oxidoreductase activity. This method combines genetic fusion constructs between glutathione transporter and redox-sensitive fluorescent protein, enabling rapid characterization of enzyme activity and structure-function relationships. The assay allows direct screening of glutaredoxin activity and efficient detection of enzyme activity with physiological substrates.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Dong Chen, Siyuan Xia, Rukang Zhang, Yuancheng Li, Christopher A. Famulare, Hao Fan, Rong Wu, Mei Wang, Allen C. Zhu, Shannon E. Elf, Rui Su, Lei Dong, Martha Arellano, William G. Blum, Hui Mao, Sagar Lonial, Wendy Stock, Olatoyosi Odenike, Michelle Le Beau, Titus J. Boggon, Chuan He, Jianjun Chen, Xue Gao, Ross L. Levine, Jing Chen
Summary: The study revealed that mutant IDH2 in AML cells commonly has K413 acetylation, which negatively regulates its activity by attenuating dimerization and blocking substrate and cofactor binding. K413 acetylation of mitochondrial mIDH2 is achieved through a series of hierarchical phosphorylation events, optimizing the leukemogenic ability of mIDH2 in AML cells.
Article
Biochemistry & Molecular Biology
Daniel C. Moreira, Debora P. Paula, Marcelo Hermes-Lima
Summary: Insect diapause involves biochemical adaptations, including reduced oxidative capacity and increased enzymatic activities in preparation for oxidative stress. These changes occur early in diapausing animals, within the first 24 hours to 20 days of diapause, indicating a complex response to metabolic depression and stress resistance in tropical insects like C. lacinia.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Fang Wu, Natali H. Muskat, Inbar Dvilansky, Omri Koren, Anat Shahar, Roi Gazit, Natalie Elia, Eyal Arbely
Summary: Lysine acetylation plays important roles in regulating metabolic enzymes, including activating or inhibiting enzyme activity, controlling posttranslational modifications, and modulating apoptotic signaling.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Lara Vogelsang, Karl-Josef Dietz
Summary: This study investigates the regeneration specificity of thiol-dependent peroxiredoxins and glutathione peroxidase-like enzymes in the cytosol of Arabidopsis thaliana cells. The results demonstrate redundancy and specificity in the redox regulatory network of the cytosol, providing insights into the role of thioredoxins in this process.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Maria Begona Gonzalez-Moro, Itziar Gonzalez-Moro, Marlon de la Pena, Jose Maria Estavillo, Pedro M. Aparicio-Tejo, Daniel Marino, Carmen Gonzalez-Murua, Izargi Vega-Mas
Summary: Nitrate and ammonium are the main nitrogen sources in agricultural soils, with ammonium playing a diverse role in plant metabolism. Different plant species have varying strategies to maintain NH4+ balance, and the study analyzed metabolic responses in seven plant species and discussed the role of different anaplerotic enzymes in ammonium nutrition.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Kyung Yeol Ko, Sun Cheol Park, So Yeon Cho, Sung-il Yoon
Summary: Spermidine is a cationic polyamine that plays important roles in bacteria, and this study provides insights into the structural features and substrate recruitment mechanism of carboxyspermidine dehydrogenase (CASDH).
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Plant Sciences
Francisco J. Corpas, Salvador Gonzalez-Gordo, Jose M. Palma
Summary: Nitric oxide (NO) and hydrogen sulfide (H2S) play key roles in plant cells by regulating protein functions through post-translational modifications, impacting cellular redox status. NADPH is essential for cell growth and development, participating in various biochemical pathways, while NADP-dependent enzymes help generate NADPH by oxidoreductases. These molecules contribute to diverse cellular processes and detoxification, emphasizing the importance of redox balance in plant cells.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Ting Zheng, Guo Wu, Xiang Tao, Bing He
Summary: This study found that SIZ1 positively regulates plant cadmium tolerance. The loss-of-function siz1-2 mutant showed impaired resistance to cadmium exposure and accumulated more reactive oxygen species (ROS). Further analysis revealed that the higher cadmium sensitivity of the siz1-2 mutant was partially restored by overexpression of GSH1.
Article
Environmental Sciences
Xiong Li, Di Chen, Ya Yang, Yuanyuan Liu, Landi Luo, Qian Chen, Yongping Yang
Summary: The study identified key genes and pathways involved in the response of turnip landraces to Cd stress through comparative transcriptome analysis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Francesco Caligiore, Erika Zangelmi, Carola Vetro, Takfarinas Kentache, Joseph P. Dewulf, Maria Veiga-da-Cunha, Emile Van Schaftingen, Guido Bommer, Alessio Peracchi
Summary: Transaminases can react with alternative substrates, although with low efficiency, and are ineffective at utilizing sugars and sugar derivatives, except for dihydroxyacetone phosphate.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Beom Sik Kang, GaneshKumar Pugalendhi, Ku-Jin Kim
Article
Biochemistry & Molecular Biology
Udeok Seo, Ku-Jin Kim, Beom Sik Kang
Article
Biochemistry & Molecular Biology
Hyo Je Cho, Hyun Joo Lee, Ha Yeon Cho, Jeen-Woo Park, Dong-Seok Lee, Hyun-Shik Lee, Oh-Shin Kwon, Beom Sik Kang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Biochemistry & Molecular Biology
Dong Kyu Kim, Hyun Joo Lee, Jiwon Kong, Ha Yeon Cho, Sunghoon Kim, Beom Sik Kang
Summary: In this study, crystal structures of the human MRS catalytic main body and the complex of the GST domain and AIMP3 were determined. The structures reveal human-specific details of MRS and provide a dynamic model for understanding the domain orientation of MRS. This study suggests a dynamic switching between two possible MRS conformations based on the position of the GST domain relative to the catalytic main body.
NUCLEIC ACIDS RESEARCH
(2021)