期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 293, 期 29, 页码 11325-11340出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA118.002441
关键词
outer membrane; lipid transport; phospholipid; membrane protein; protein complex; lipid asymmetry; membrane channel; MlaA; OmpC; osmoporin
资金
- National University of Singapore Graduate School for Integrative Sciences and Engineering scholarship
- Singapore Ministry of Education Academic Research Fund Tier 3 Grant [MOE2012-T3-1-008]
- National University of Singapore start-up funding
- Singapore Ministry of Education Academic Research Fund Tier 1 Grant [MOE2013-T2-1-148]
- Singapore Ministry of Education Academic Research Fund Tier 2 Grant [MOE2013-T2-1-148]
A distinctive feature of the Gram-negative bacterial cell envelope is the asymmetric outer membrane (OM), where lipopolysaccharides and phospholipids (PLs) reside in the outer and inner leaflets, respectively. This unique lipid asymmetry renders the OM impermeable to external insults, including antibiotics and bile salts. In Escherichia coli, the complex comprising osmoporin OmpC and the OM lipoprotein MlaA is believed to maintain lipid asymmetry by removing mislocalized PLs from the outer leaflet of the OM. How this complex performs this function is unknown. Here, we defined the molecular architecture of the OmpC-MlaA complex to gain insights into its role in PL transport. Using in vivo photo-cross-linking and molecular dynamics simulations, we established that MlaA interacts extensively with OmpC and is located entirely within the lipid bilayer. In addition, MlaA forms a hydrophilic channel, likely enabling PL translocation across the OM. We further showed that flexibility in a hairpin loop adjacent to the channel is critical in modulating MlaA activity. Finally, we demonstrated that OmpC plays a functional role in maintaining OM lipid asymmetry together with MlaA. Our work offers glimpses into how the OmpC-MlaA complex transports PLs across the OM and has important implications for future antibacterial drug development.
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