Article
Developmental Biology
Farah Saad, David R. Hipfner
Summary: The Hedgehog (Hh) pathway is regulated by G protein-coupled receptors (GPCRs) in Drosophila, with Mthl5 identified as a modulator of this pathway. This suggests potential crosstalk between GPCRs and the Hh pathway in mammals as well.
Review
Plant Sciences
Yijie Wang, Jose Ramon Botella
Summary: As sessile organisms, plants have evolved mechanisms to adapt to environmental fluctuations. Heterotrimeric G proteins play essential roles in the response to stress in plants, regulating multiple signaling pathways. Understanding the functions of G proteins in stress reactions can contribute to the development of resilient crops and global food security.
Article
Microbiology
Yuchang Di, Siyue Xu, Mingzhe Chi, Youwei Hu, Xiao Zhang, Honghai Wang, Wenhong Zhang, Xuelian Zhang
Summary: The acetylation of CRP K193 in Mycobacterium tuberculosis (Mtb) modulates its DNA-binding ability, affects mycobacterial growth and stress response, and regulates the expression of relevant genes. The reversible acetylation of CRP K193 is mediated by AcP and NAD(+)-dependent deacetylases. These findings contribute to a better understanding of the survival and pathogenicity of mycobacteria.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biochemistry & Molecular Biology
Natsumi Maruta, Yuri Trusov, Alan M. Jones, Jose R. Botella
Summary: Plant G proteins consist of canonical G alpha subunits and unconventional XLG subunits, interacting with G beta gamma dimers and RGS protein, and participating in development, stress responses, and immunity. Despite the overall similarity between plant and animal G alpha subunits, they convey signals differently.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Amy M. Chinn, Cristina Salmeron, Jihyung Lee, Krishna Sriram, Eyal Raz, Paul A. Insel
Summary: This study investigates the regulation of cellular cAMP concentrations in dendritic cells (DCs) and identifies phosphodiesterase 4B (PDE4B) as a homeostatic regulator of cAMP levels. The findings suggest that targeting PDE4B could potentially be used to treat conditions with low cellular cAMP concentrations, including Th2-allergic asthma.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Endocrinology & Metabolism
Jurgen Wess
Summary: GPCRs are significant targets for drugs approved by the US FDA, where activation of different GPCRs can impact physiological outcomes differently, reflecting the effects of G protein activation from different perspectives.
Article
Plant Sciences
Genki Chaya, Shuhei Segami, Moeka Fujita, Yoichi Morinaka, Yukimoto Iwasaki, Kotaro Miura
Summary: This study reveals that both DEP1 and OsGGC2 genes are positive regulators of plant height and their functions are redundant.
Review
Biochemistry & Molecular Biology
Choel Kim, Rajesh Sharma
Summary: The interaction between the C-terminal catalytic domain and the N-terminal regulatory domain of cGMP-dependent protein kinases inhibits the intrinsic activity of the enzyme. Selective binding of cGMP to specific domains within the regulatory domain disrupts this inhibitory interaction, leading to activation of the enzyme. Crystal structures of different domains reveal specific contacts and conformational changes that explain the selectivity and activation of the enzyme by cyclic nucleotides.
Article
Cell Biology
Marion Laudette, Yannis Sainte-Marie, Gregoire Cousin, Dorian Bergonnier, Ismahane Belhabib, Stephanie Brun, Karina Formoso, Loubna Laib, Florence Tortosa, Camille Bergoglio, Bertrand Marcheix, Jan Boren, Olivier Lairez, Jeremy Fauconnier, Alexandre Lucas, Jeanne Mialet-Perez, Cedric Moro, Frank Lezoualc'h
Summary: The study reveals that dietary saturated fatty acid palmitate increases cAMP synthesis, disrupting lipid metabolism balance in cardiomyocytes and leading to mitochondrial dysfunction and ultimately cardiomyocyte death. Epac1 plays a crucial role in this process by modulating mitochondrial FA uptake and oxidation, with potential implications in cardiac pathophysiology.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Chiaki Yamamoto, Fumio Takahashi, Yosuke Ooe, Haruto Shirahata, Aika Shibata, Masahiro Kasahara
Summary: A novel adenylyl cyclase/cAMP phosphodiesterase gene named CAPE was isolated from liverworts and gymnosperms. The distribution of CAPE in plants reproducing via motile sperm corresponded to evolutionary history, suggesting its potential involvement in sexual reproduction process using motile sperm in streptophytes.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Brian D. Young, Jihui Sha, Ajay A. Vashisht, James A. Wohlschlegel
Summary: This study identified novel regulators of G beta gamma signaling in human cells using mass spectrometry, with KCTD2 and KCTD5 shown to specifically recognize G beta gamma and be involved in downstream signal transduction. These KCTD proteins act as substrate adaptors for a multisubunit CUL3-RING ubiquitin ligase, promoting monoubiquitination of G beta(1/2) lysine-23 and regulating downstream signaling. Depletion of these adaptors impairs downstream signaling in cancer cell lines.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Plant Sciences
Aloysius Wong, Wei Chi, Jia Yu, Chuyun Bi, Xuechen Tian, Yixin Yang, Chris Gehring
Summary: This article provides a historical overview and recent advances in adenylate cyclases (ACs) and cAMP signaling in plants, including the exploration of the active AC functions within multifunctional protein complexes.
Article
Biochemistry & Molecular Biology
Lian Wu, Xiaodong Wang, Zhiwen Yu, Xin Cui, Quan Xu
Summary: This paper reveals the antagonistic regulation of grain yield and quality by two type C G protein gamma subunits, DEP1 and GS3, in rice. Editing the DEP1 gene increases grain number per panicle but has negative effects on taste value, texture properties, and chalkiness-related traits. Editing the GS3 gene decreases grain number per panicle but increases grain length and improves taste value, appearance, texture properties, and Rapid Visco Analyser (RVA) profiles. Molecular design breeding combining gs3 and dep1 alleles further increases grain yield, enhances texture properties and RVA profiles, and improves taste quality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Yoichiro Kamimura, Masahiro Ueda
Summary: Chemotaxis is the process of cells undergoing directional movement along ambient chemical gradients, playing important roles in human physiology and pathology. Studies on Dictyostelium cells have elucidated the crucial roles of various mechanisms of GPCR signaling in broad range sensing of chemical gradients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Khem Raj Ghusinga, Timothy C. Elston, Alan M. Jones
Summary: G-proteins are molecular switches that transmit extracellular signals to intracellular targets. In plants, the role of nucleotide exchange in this process is less critical, but under dynamic conditions, the loss of RGS results in a strong phenotype.
Article
Endocrinology & Metabolism
Alta Berger, Ahmed Kablan, Catherine Yao, Thuy Ho, Brandon Podyma, Lee S. Weinstein, Min Chen
Article
Medicine, Research & Experimental
Min Chen, Yogendra B. Shrestha, Brandon Podyma, Zhenzhong Cui, Benedetta Naglieri, Hui Sun, Thuy Ho, Eric A. Wilson, Yong-Qi Li, Oksana Gavrilova, Lee S. Weinstein
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Biochemistry & Molecular Biology
Michael P. Guy, Brandon M. Podyma, Melanie A. Preston, Hussam H. Shaheen, Kady L. Krivos, Patrick A. Limbach, Anita K. Hopper, Eric M. Phizicky
Article
Endocrinology & Metabolism
Min Chen, Eric A. Wilson, Zhenzhong Cui, Hui Sun, Yogendra B. Shrestha, Brandon Podyma, Christina H. Le, Benedetta Naglieri, Karel Pacak, Oksana Gavrilova, Lee S. Weinstein
MOLECULAR METABOLISM
(2019)
Article
Biology
Brandon Podyma, Dove-Anna Johnson, Laura Sipe, Thomas Parks Remcho, Katherine Battin, Yuxi Liu, Sung Ok Yoon, Christopher D. Deppmann, Ali Deniz Guler
Article
Endocrinology & Metabolism
Eric A. Wilson, Hui Sun, Zhenzhong Cui, Marshal T. Jahnke, Mritunjay Pandey, Peter Metzger, Oksana Gavrilova, Min Chen, Lee S. Weinstein
Summary: The study highlights the importance of the G-protein subunits G(q)alpha and G(11)alpha in the dorsomedial hypothalamus for regulating energy balance, expenditure, thermoregulation, BAT function, and WAT browning.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Health Care Sciences & Services
Benjamin A. Y. Cher, Eric A. Wilson, Alexa M. Pinsky, Ryan F. Townshend, Ann Wolski, Michael Broderick, Allison M. Milen, Audrey Lau, Amrit Singh, Sandro K. Cinti, Carl G. Engelke, Anjan K. Saha
Summary: The study described and evaluated a COVID-19 telephone triage hotline used by a large academic medical center in the midwestern United States. During the study period, a total of 3929 calls were received, with an average of 91 calls per day, peaking in the early morning and evening hours. Callers were most often directed to the online patient portal (42%), nursing hotlines (34%), or employee health services (18%).
JOURNAL OF MEDICAL INTERNET RESEARCH
(2021)
Review
Radiology, Nuclear Medicine & Medical Imaging
Bradley Carlson, Carla Harmath, Kiran Turaga, Hedy L. Kindler, Samuel G. Armato, Christopher Straus
Summary: This article reviews the literature on imaging modalities for MPeM and analyzes the clinical efficacy of different imaging techniques and common imaging features of MPeM. The study found that CT was the most commonly used imaging modality, but PET/CT and MRI showed promising results. Key features that can differentiate MPeM from other diseases were identified. The article suggests that MPeM imaging reports should focus on highlighting these key features for better data aggregation.
ABDOMINAL RADIOLOGY
(2022)