期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 293, 期 21, 页码 8032-8047出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA118.003200
关键词
pancreas; oxidative stress; mitochondrial permeability transition (MPT); cyclophilin D; bioenergetics; reactive oxygen species (ROS); apoptosis; necrosis (necrotic death); antioxidant; Acute Pancreatitis; Seahorse
资金
- Wellcome Trust [102381/Z/13/Z]
- Medical Research Council
- China Scholarship Council
- National Institute for Health
- MRC [MR/K012967/1] Funding Source: UKRI
Mitochondrial dysfunction lies at the core of acute pancreatitis (AP). Diverse AP stimuli induce Ca2+-dependent formation of the mitochondrial permeability transition pore (MPTP), a solute channel modulated by cyclophilin D (CypD), the formation of which causes ATP depletion and necrosis. Oxidative stress reportedly triggers MPTP formation and is elevated in clinical AP, but how reactive oxygen species influence cell death is unclear. Here, we assessed potential MPTP involvement in oxidant-induced effects on pancreatic acinar cell bioenergetics and fate. H2O2 application promoted acinar cell apoptosis at low concentrations (1-10 m), whereas higher levels (0.5-1 mm) elicited rapid necrosis. H2O2 also decreased the mitochondrial NADH/FAD(+) redox ratio and (m) in a concentration-dependent manner (10 m to 1 mm H2O2), with maximal effects at 500 m H2O2. H2O2 decreased the basal O-2 consumption rate of acinar cells, with no alteration of ATP turnover at <50 m H2O2. However, higher H2O2 levels (50 m) diminished spare respiratory capacity and ATP turnover, and bioenergetic collapse, ATP depletion, and cell death ensued. Menadione exerted detrimental bioenergetic effects similar to those of H2O2, which were inhibited by the antioxidant N-acetylcysteine. Oxidant-induced bioenergetic changes, loss of (m), and cell death were not ameliorated by genetic deletion of CypD or by its acute inhibition with cyclosporine A. These results indicate that oxidative stress alters mitochondrial bioenergetics and modifies pancreatic acinar cell death. A shift from apoptosis to necrosis appears to be associated with decreased mitochondrial spare respiratory capacity and ATP production, effects that are independent of CypD-sensitive MPTP formation.
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