Article
Immunology
Xin Zhang, Siting Wu, Zetian Liu, Hong Chen, Jiaming Liao, Jingguang Wei, Qiwei Qin
Summary: In this study, the researchers cloned and characterized the RIP2 homolog from orange-spotted grouper (EcRIP2) and investigated its role in fish DNA virus infection. They found that EcRIP2 could inhibit the replication of SGIV and suppress the expression of inflammatory factors by competing with EcASC for binding EcCaspase-1. This study provides new insights into the modulatory mechanism of the RIP2-associated pathway and RIP2-mediated fish diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Yuexuan Wang, Suifeng Xu, Chengzong Han, Liqun Wang, Qi Zheng, Shaowen Wang, Youhua Huang, Shina Wei, Qiwei Qin
Summary: SGIV infection, a major cause of mortality and economic losses in grouper aquaculture industry, can be effectively inhibited by curcumin through multiple antiviral mechanisms both in vitro and in vivo. Curcumin impairs attachment of SGIV virion and regulates cellular immune and inflammatory responses related to the NF-kappa B signaling pathway. Additionally, curcumin plays a critical role in preventing SGIV infection through enhancing antioxidant capacity via the Nrf2/Keap1 signaling pathway. This study highlights the potential of curcumin as a candidate drug for treating SGIV infection.
Article
Fisheries
Minglan Guo, Jingguang Wei, Yongcan Zhou, Qiwei Qin
Summary: In this study, a protein called EcMKP-1 was cloned and found to play a critical role in combating Singapore grouper iridovirus (SGIV) infection. It regulates the host's immune response and suppresses viral replication. The results demonstrate the important functions of EcMKP-1 in antiviral immunity, JNK dephosphorylation, and anti-apoptosis during SGIV infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Yuexuan Wang, Suifeng Xu, Chengzong Han, Youhua Huang, Jingguang Wei, Shina Wei, Qiwei Qin
Summary: The molecular mechanism of curcumin against SGIV infection mainly includes suppressing cell apoptosis, inhibiting related signaling pathways, and promoting autophagy.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Suifeng Xu, Yuexuan Wang, Chengzong Han, Yunxiang Jiang, Qiwei Qin, Shina Wei
Summary: The study revealed that cystatin F (CyF) plays a crucial role in fish virus infection by participating in innate immune responses. It inhibits the expression of viral genes and regulates apoptosis, as well as the production of inflammatory factors. CyF also modulates the activity of promoters related to interferon and NF-kappa B. These findings highlight the importance of CyF in DNA virus infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jinpeng Chen, Liqun Wang, Jianling Huang, Xinshuai Li, Lingfeng Guan, Qing Wang, Min Yang, Qiwei Qin
Summary: This study cloned and characterized a novel type of MHC-I genotype, named EcMHC-IA*01, which plays an important role in antiviral immune response and apoptosis in fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Hong Zhang, Zongwei Guo, Yafei Guo, Ziqian Wang, Yao Tang, Ting Song, Zhichao Zhang
Summary: The research showed that the transfer of Bim between Bcl-2-like protein and Hsp70 underlies the crosstalk in mitochondrial apoptosis pathway, leading to apoptosis in cancer cell lines by inhibiting free Bim and facilitating oncogenic client AKT folding and activation.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Fisheries
Suifeng Xu, Yuexuan Wang, Yunxiang Jiang, Chengzong Han, Qiwei Qin, Shina Wei
Summary: Cystatin A (CyA) in orange-spotted grouper plays a crucial role in fish virus infection by participating in innate immune response, regulating viral gene expression, and promoting apoptosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Virology
Yuexuan Wang, Honglin Han, Kecheng Zhu, Suifeng Xu, Chengzong Han, Yunxiang Jiang, Shina Wei, Qiwei Qin
Summary: In this study, Ec-Cathepsin D (Ec-CD) was identified from the orange-spotted grouper and its function in SGIV infection was investigated. The overexpression of Ec-CD inhibited SGIV replication, SGIV-induced apoptosis, and pro-inflammatory cytokine production. It also suppressed the activation of MAPK pathways.
Article
Oncology
Jordy C. G. van der Zwet, Jessica G. C. A. M. Buijs-Gladdines, Valentina Cordo', Donna O. Debets, Willem K. Smits, Zhongli Chen, Jelle Dylus, Guido J. R. Zaman, Maarten Altelaar, Koichi Oshima, Beat Bornhauser, Jean-Pierre Bourquin, Jan Cools, Adolfo A. Ferrando, Josef Vormoor, Rob Pieters, Britta Vormoor, Jules P. P. Meijerink
Summary: Activation of IL7R signaling contributes to steroid resistance in pediatric T-ALL patients through phosphorylation of BIM, which can be restored by MEK inhibitors. Selumetinib shows synergistic effects with steroids in both IL7-dependent and IL7-independent steroid resistant T-ALL PDX samples, highlighting its potential as a treatment option.
Article
Biology
Peng Cheng, Yuzhu Hou, Mingxing Bian, Xueru Fang, Yan Liu, Yuanfang Rao, Shuo Cao, Yanjun Liu, Shuai Zhang, Yanke Chen, Xu Dong, Zhu Liu
Summary: This study reveals the molecular mechanism by which Ub inhibits BAK, showing that Ub occludes the binding of prodeath activators in the hydrophobic groove of BAK, affecting BAK activation and membrane permeabilization. Weakening the interaction between Ub and BAK allows BID to activate ubiquitinated BAK.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ravit Malishev, Shani Ben-Zichri, Ofek Oren, Nitzan Shauloff, Tal Peretz, Ran Taube, Niv Papo, Raz Jelinek
Summary: The BIM-BH3 motif is a key pro-apoptotic domain that forms toxic amyloid fibrils, leading to cell death and impacting mitochondrial functions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Hong Zhang, Ting Song, Ziqian Wang, Yafei Guo, Hang Wang, Qishuang Gao, Zhichao Zhang, Uwituze Laura Bonneete
Summary: By using a specific inhibitor of Hsp70/Bim complex and knocking down Bcr-Abl, researchers have found that Bcr-Abl can prevent cell apoptosis by driving the formation of Hsp70/Bim complex. Additionally, Bcr-Abl can stabilize oncogenic clients by driving the formation of Hsp70/Bim complex.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Virology
Pin-Han Chen, Tsai-Ching Hsueh, Jen-Leih Wu, Jiann-Ruey Hong
Summary: This study demonstrates that infectious spleen and kidney necrosis virus (ISKNV) infection can induce host cell death through the regulation of mitochondrial membrane potential. The dynamic interaction between pro-apoptotic and anti-apoptotic members of the Bcl-2 family plays a crucial role in this process.
Article
Virology
Yiqun Li, Yuding Fan, Yong Zhou, Nan Jiang, Mingyang Xue, Yan Meng, Wenzhi Liu, Jingjing Zhang, Ge Lin, Lingbing Zeng
Summary: The study showed that AdBcl-xL protein level was significantly reduced during GSIV infection, and it played a negative role by binding to AdBak and inhibiting p53 activation, thus preventing GSIV-induced mitochondrial apoptosis and virus replication.
Article
Biochemistry & Molecular Biology
Leah Fitzsimmons, Rachel Cartlidge, Catherine Chang, Nenad Sejic, Laura C. A. Galbraith, Chathura D. Suraweera, Deborah Croom-Carter, Grant Dewson, Rosemary J. Tierney, Andrew Bell, Clare Shannon-Lowe, Marco J. Herold, Alan B. Rickinson, Peter M. Colman, David C. S. Huang, Andreas Strasser, Marc Kvansakul, Martin Rowe, Gemma L. Kelly
CELL DEATH AND DIFFERENTIATION
(2020)
Review
Biochemistry & Molecular Biology
Suresh Banjara, Chathura D. Suraweera, Mark G. Hinds, Marc Kvansakul
Article
Biochemistry & Molecular Biology
Chathura D. Suraweera, Mohd Ishtiaq Anasir, Srishti Chugh, Airah Javorsky, Rachael E. Impey, Mohammad Hasan Zadeh, Tatiana P. Soares da Costa, Mark G. Hinds, Marc Kvansakul
Article
Biochemistry & Molecular Biology
Chathura D. Suraweera, Denis R. Burton, Mark G. Hinds, Marc Kvansakul
Article
Biochemistry & Molecular Biology
Suresh Banjara, Jaison D. Sa, Mark G. Hinds, Marc Kvansakul
BIOCHEMICAL JOURNAL
(2020)
Article
Multidisciplinary Sciences
Nikolay Popgeorgiev, Jaison D. Sa, Lea Jabbour, Suresh Banjara, Trang Thi Minh Nguyen, Aida Akhavan-E-Sabet, Rudy Gadet, Nikola Ralchev, Stephen Manon, Mark G. Hinds, Hans-Jurgen Osigus, Bernd Schierwater, Patrick O. Humbert, Ruth Rimokh, Germain Gillet, Marc Kvansakul
Article
Biochemistry & Molecular Biology
Chathura D. Suraweera, Mark G. Hinds, Marc Kvansakul
BIOCHEMICAL JOURNAL
(2020)
Review
Microbiology
Chathura D. Suraweera, Mark G. Hinds, Marc Kvansakul
Summary: The article discusses the two forms of apoptosis and the role of the Bcl-2 gene family in intrinsic apoptosis. It also explores how viral Bcl-2 genes interact with the host immune system to facilitate viral infection and propagation.
Article
Biochemistry & Molecular Biology
Jing Yuan How, Rebecca K. Stephens, Krystle Y. B. Lim, Patrick O. Humbert, Marc Kvansakul
Summary: Scribble is a critical cell polarity regulator, and mutations in it lead to neural tube defects. Research shows that these mutations not only affect binding to Vangl2, but also disrupt binding to multiple other signaling regulators.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Chathura D. Suraweera, Suresh Banjara, Mark G. Hinds, Marc Kvansakul
Summary: The B-cell lymphoma-2 (Bcl-2) family of genes regulates intrinsic apoptosis by coordinating the integrity of the mitochondrial outer membrane. Bcl-2 genes are present in early metazoans and have different variations among species. The structure of Bcl-2 proteins is highly conserved over evolutionary time, and some Bcl-2 proteins may have non-apoptotic functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Airah Javorsky, Patrick O. Humbert, Marc Kvansakul
Summary: Scribble is a crucial regulator of cell polarity during viral infections. The NS1 protein of influenza virus can target Scribble PDZ domains and disrupt the normal control of cell polarity. The study characterizes the binding affinities of different NS1 PDZ binding motifs to Scribble PDZ domains and provides insights into the structural basis of their interactions.
Article
Virology
Chathura D. Suraweera, Mark G. Hinds, Marc Kvansakul
Summary: Apoptosis is a defense mechanism used by multicellular organisms to counteract viral infection. Viruses have evolved various countermeasures to ensure cell viability and optimize their replication. In this study, the crystal structures of Epstein-Barr virus (EBV)-encoded BHRF1 bound to proapoptotic proteins Bid and Puma were determined. BHRF1 engages with BH3 peptides using its Bcl-2 fold and mimics the canonical ionic interaction seen in host Bcl-2:BH3 complexes. Furthermore, Bid and Puma utilize a distinct hydrophobic interaction with BHRF1. These findings provide insights into EBV-mediated inhibition of host cell apoptosis and the flexibility of virus-encoded Bcl-2 proteins in mimicking key interactions from the host signaling pathways.