期刊
GENOMICS
卷 105, 期 5-6, 页码 296-303出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2015.01.010
关键词
Cucumis sativus; DNA-binding proteins; Gene regulatory networks; Transcription factors; Transcriptome; Trichomes
资金
- China 973 Program [2012CB113900]
- National Natural Science Foundation of China [31271291, 31471156]
- Shanghai Municipal Committee of Science and Technology [13JC1403600]
- Specialized Research Fund for the Doctoral Program of Higher Education [20120073110051]
The regulatory gene network of unicellular trichome development in Arabidopsis thaliana has been studied intensively, but that of multicellular remains unclear. In the present study, we characterized cucumber trichomes as representative multicellular and unbranched structures, but in a spontaneous mutant, mict (micro-trichome), all trichomes showed a micro-size and stunted morphologies. We revealed the transcriptome profile using Illumina HiSeq 2000 sequencing technology, and determined that a total of 1391 genes exhibited differential expression. We further validated the accuracy of the transcriptome data by RT-qPCR and found that 43 genes encoding critical transcription factors were likely involved in multicellular trichome development. These 43 candidate genes were subdivided into seven groups: homeodomain, MYB-domain, WRKY-domain, bHLH-domain, ethylene-responsive, zinc finger and other transcription factor genes. Our findings also serve as a powerful tool to further study the relevant molecular networks, and provide a new perspective for investigating this complex and species-specific developmental process. (C) 2015 Elsevier Inc. All rights reserved.
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