Article
Reproductive Biology
Heather L. Chaney, Lindsay F. Grose, Gilles Charpigny, Susanta K. Behura, I. Martin Sheldon, James G. Cronin, Patrick Lonergan, Thomas E. Spencer, Daniel J. Mathew
Summary: This study identified biologically relevant IFNT-stimulated genes in specific endometrial cell types. The findings provide critical information on the effects of conceptus IFNT on specific endometrial compartments during early developmental processes in cattle.
BIOLOGY OF REPRODUCTION
(2021)
Review
Agriculture, Dairy & Animal Science
Cecilia Constantino Rocha, Juliano Coelho Da Silveira, Niamh Forde, Mario Binelli, Guilherme Pugliesi
Summary: This review focuses on the innate immune events modulated by conceptus signaling during early pregnancy in ruminants, with a special emphasis on the role of IFN-tau in regulating the immune system to prevent conceptus rejection.
ANIMAL REPRODUCTION
(2021)
Article
Cell Biology
Gong-Xue Jia, Wen-Ji Ma, Zhao-Bo Wu, Shuang Li, Xiao-Qian Zhang, Zhen He, Shi-Xin Wu, Hai-Ping Tao, Yi Fang, Yong-Wu Song, Shang-Rong Xu, Xiao-Qun Wang, Qi-En Yang
Summary: Using single-cell RNA sequencing, we investigated the transcriptomic dynamics of sheep conceptus and corresponding endometrium during pre- and peri-implantation stages. The study identified distinct cell types in spherical blastocysts and elongated conceptuses, revealing cell fate specifications. Additionally, the molecular responses of 13 endometrium-derived cell types to conceptus development were elucidated. Furthermore, paired transcripts involved in the communication between extraembryonic membrane and endometrium were identified.
Article
Reproductive Biology
A. K. Talukder, M. B. Rabaglino, J. A. Browne, G. Charpigny, P. Lonergan
Summary: This study aimed to investigate the dose- and time-dependent relationship between IFNT and the expression of ISGs in the endometrium, as well as its impact on the maternal recognition of pregnancy in cattle. The results indicated that IFNT acts on the uterus in a dose- and time-dependent manner, and timely exposure to sufficient IFNT is crucial for the establishment of successful pregnancy.
Article
Multidisciplinary Sciences
Gerhard A. Burger, Daphne N. Nesenberend, Carlijn M. Lems, Sander C. Hille, Joost B. Beltman
Summary: Epithelial-mesenchymal transition (EMT) and upregulation of programmed death-ligand 1 (PD-L1) play crucial roles in cancer progression, but their molecular interaction remains unclear. In this study, a mathematical model was proposed to describe the crosstalk between EMT and interferon gamma (IFN gamma)-induced PD-L1 expression. The model revealed that microRNA-200 (miR-200) regulates the stability of the EMT circuit and PD-L1 levels, which are further enhanced by IFN gamma. This bidirectional crosstalk also suggests that IFN gamma promotes EMT and inhibits mesenchymal-epithelial transition (MET). Overall, this model provides insights into the mechanisms underlying EMT-mediated immune evasion and resistance to immunotherapy.
ROYAL SOCIETY OPEN SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Bethany E. Liebig, Jeanette V. Bishop, Kevin D. McSweeney, Hana Van Campen, Carolina L. Gonzalez-Berrios, Thomas R. Hansen, Milton G. Thomas
Summary: The objective of this study was to determine if sorting Holstein dairy cows based on genetic markers and reproductive records would improve pregnancy recognition signaling. The results showed that cows with high genetic values and fewer services per conception had longer conceptuses and enhanced maternal recognition of pregnancy.
APPLIED ANIMAL SCIENCE
(2022)
Article
Biochemical Research Methods
Yingying Ma, Songbiao Zhu, Meiqi Yi, Wenhao Zhang, Yuanyuan Xue, Xiaohui Liu, Haiteng Deng
Summary: Protein S-glutathionylation is an important post-translational modification that regulates cellular processes. This study investigated the changes in glutathionylome during epithelial-mesenchymal transition (EMT) and found that the glutathionylation level of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) increased, inhibiting its enzymatic activity and promoting EMT.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Veterinary Sciences
Haokun Liu, Caixia Wang, Zuhui Li, Chunmei Shang, Xinyan Zhang, Ruixue Zhang, Aihua Wang, Yaping Jin, Pengfei Lin
Summary: IFNT treatment upregulated the expression of STAT family members and increased the levels of p-STAT1 and p-STAT3 in goat endometrial epithelial cells. In vivo experiments confirmed the significant increase in mRNA and protein phosphorylation levels of STAT1 and STAT3 in the uterus on day 18 of pregnancy. The spatial and temporal changes in the expression of STAT1/3 during embryo implantation in the goat endometrium were also highlighted.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Neurosciences
Ying Chen, Hui Xu, Pei Yu, Qing Wang, Shenggang Li, Fufu Ji, Chunwang Wu, Qing Lan
Summary: Previous studies have shown that high expression of IFI30 is associated with glioma and tumor grade. In this study, it was found that IFI30 promotes the epithelial-mesenchymal transition (EMT) process in glioma cells through the activation of the EGFR/AKT/GSK3 beta/beta-catenin pathway. Additionally, IFI30 regulates the chemoresistance of glioma cells to temozolomide by affecting the expression of the transcription factor Slug. These findings indicate that IFI30 may serve as a prognostic marker and a potential therapeutic target for temozolomide-resistant glioma.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Multidisciplinary Sciences
Michela Serresi, Sonia Kertalli, Lifei Li, Matthias Jurgen Schmitt, Yuliia Dramaretska, Jikke Wierikx, Danielle Hulsman, Gaetano Gargiulo
Summary: Research has shown that chromatin regulators have a broader impact on EMT interconversion in lung cancer cells than kinases, and the loss of ARID1A, DOT1L, BRD2, and ZMYND8 may have nondeterministic and sometimes opposite consequences. These findings reveal general principles underlying transcriptional control of cancer cell plasticity.
Article
Reproductive Biology
Li-Ge Bu, Ya Sun, Ting-Yue Li, Li-Li Kong, Hao-Nan Yu, Shi-Jie Li, Nai-Zheng Ding, Hua Ni
Summary: In this study, the expression and regulation of ITGB8 in goat uterus were investigated, and the miR-187/ITGB8 axis was found to be potentially involved in conceptus attachment in response to interferon tau.
Article
Biochemical Research Methods
Ayalur Raghu Subbalakshmi, Sarthak Sahoo, Prakruthi Manjunatha, Shaurya Goyal, Vignesh A. Kasiviswanathan, Yeshwanth Mahesh, Soundharya Ramu, Isabelle McMullen, Jason A. A. Somarelli, Mohit Kumar Jolly
Summary: ELF3 is a factor strongly associated with an epithelial phenotype and is inhibited during EMT. It inhibits the progression of EMT and can counteract EMT induction even in the presence of EMT-inducing factors. The prognostic capacity of ELF3 is specific to cell-of-origin or lineage.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Article
Agriculture, Dairy & Animal Science
Ayami Yamada, Kaito Ohtsuki, Natsumi Shiga, Jonathan A. Green, Yuta Matsun, Kazuhiko Imakawa
Summary: This study found that in ruminant ungulates, the formation of binucleated trophoblast cells begins after the initial contact of the conceptus with the maternal endometrium, followed by epithelial-mesenchymal transition (EMT) in trophoblast cells. These sequential events are necessary for establishing pregnancy in ruminants.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Oncology
Ana Pavlic, Kristian Urh, Katarina Stajer, Emanuela Bostjancic, Nina Zidar
Summary: The study analyzed the expression of the miR-200 family as EMT markers and their target genes in colorectal carcinoma. It found partial EMT at the invasive tumor front and partial MET in metastases based on miR-200 family and its target genes expression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Reproductive Biology
Megan A. Mezera, Wenli Li, Milo C. Wiltbank
Summary: The study found significant alterations in expression of interferon-stimulated genes (ISGs) during early pregnancy, with immune/interferon signaling pathways being enriched. Most ISGs decreased during the second month of pregnancy, but some remained elevated even after cessation of IFNT secretion. Additionally, the study highlighted the importance of lack of PGF exposure in corpus luteum maintenance during early pregnancy, while the roles of diverse ISGs and other pathways activated during early pregnancy remain undefined.
BIOLOGY OF REPRODUCTION
(2021)
Review
Biochemistry & Molecular Biology
Hiroshi Fujiwara, Masanori Ono, Yukiyasu Sato, Kazuhiko Imakawa, Takashi Iizuka, Kyosuke Kagami, Tomoko Fujiwara, Akihito Horie, Hirohiko Tani, Akira Hattori, Takiko Daikoku, Yoshihiko Araki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Developmental Biology
Keigo Nakamura, Kazuya Kusama, Atsushi Ideta, Kazuhiko Imakawa, Masatoshi Hori
Review
Biochemistry & Molecular Biology
Keigo Nakamura, Kazuya Kusama, Yoshihito Suda, Hiroshi Fujiwara, Masatoshi Hori, Kazuhiko Imakawa
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Mohammad A. Zinnah, Mohamed A. Marey, Ihshan Akhtar, Ibrahim F. Elesh, Yuta Matsuno, Ahmed E. Elweza, Dongxue Ma, Mariani Fiorenza, Motoki Sasaki, Masayuki Shimada, Kazuhiko Imakawa, Akio Miyamoto
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Nagaki Ohnuki, Tomoko Kobayashi, Misaki Matsuo, Kohei Nishikaku, Kazuya Kusama, Yasushi Torii, Yasuko Inagaki, Masatoshi Hori, Kazuhiko Imakawa, Yorifumi Satou
Summary: Bovine leukemia virus (BLV) is an oncogenic retrovirus that induces enzootic bovine leukosis (EBL), and the associations between virus and host genome are not fully understood. The study used DNA-capture-seq to identify BLV integration sites, revealing oligoclonal integrations, supporting the understanding of BLV infection mechanisms.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Mariani F. Fiorenza, Mohamed A. Marey, Mohammad B. Rashid, Mohammad A. Zinnah, Dongxue Ma, Vernadyn A. Morillo, Kazuya Kusama, Masayuki Shimada, Kazuhiko Imakawa, Alfredo Q. Antoniazzi, Akio Miyamoto
Summary: The study shows that D7 bovine embryos can directly induce anti-inflammatory responses in PMNs, mainly through upregulation of ISGs expression, and PMNs can amplify and transfer embryo signals to a new PMN population, partly mediated by IFNA. This novel immunological crosstalk may contribute to embryo tolerance and pregnancy establishment in cattle.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Developmental Biology
Yuta Matsuno, Yahia A. Amin, Kazuya Kusama, Kazuhiko Imakawa
Summary: In this study, new candidate molecules for maintaining conceptus adhesion in ruminants, specifically fibrin, were identified through RNA-sequence analysis and iTRAQ analysis. These molecules, including coagulation factors and fibrinolysis factors, play a crucial role in the conceptus adhesion process. This research sheds light on the mechanisms underlying conceptus adhesion in ruminants.
Article
Genetics & Heredity
Kazuya Kusama, Mohammad B. Rashid, Rasoul Kowsar, Mohamed A. Marey, Anup K. Talukder, Kentaro Nagaoka, Masayuki Shimada, Hasan Khatib, Kazuhiko Imakawa, Akio Miyamoto
Summary: This study characterized proteins and exosomal microRNAs (miRNAs) in the uterine flushings (UF) of cows associated with Day 7 (D7) pregnancy. The presence of multiple D7 embryos led to significant changes in the protein composition and exosomal miRNA contents of UF, potentially mediating innate immunological interactions in cows.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Keigo Nakamura, Kazuya Kusama, Masatoshi Hori, Kazuhiko Imakawa
Summary: The study revealed that bta-miR-26b derived from intrauterine EVs contributes to the down-regulation of the maternal immune system, facilitating conceptus implantation to the uterine endometrium. Furthermore, intrauterine EVs extracted from P20 UFs could regulate neutrophils to modulate endometrial immune and inflammatory responses for implanting conceptuses.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yuta Matsuno, Kazuya Kusama, Kazuhiko Imakawa
Summary: This study characterized the expression profile of lncRNAs during peri-implantation periods in sheep conceptuses. The analysis identified a total of 8808 lncRNAs, including 3423 novel lncRNAs. Gene ontology analysis revealed enrichment of lncRNA target genes in the respiratory electron transport chain (RETC). The findings suggest that lncRNAs may play a role in regulating RETC during conceptus development in sheep.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Cell Biology
Farnaz Oghbaei, Reza Zarezadeh, Davoud Jafari-Gharabaghlou, Minoo Ranjbar, Mohammad Nouri, Amir Fattahi, Kazuhiko Imakawa
Summary: The epithelial to mesenchymal transition (EMT) is crucial for successful embryo implantation and is regulated by various factors. This review aims to summarize the influencing factors on the EMT process during implantation.
CELL AND TISSUE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Ayami Yamada, Kaito Ohtsuki, Natsumi Shiga, Jonathan A. Green, Yuta Matsun, Kazuhiko Imakawa
Summary: This study found that in ruminant ungulates, the formation of binucleated trophoblast cells begins after the initial contact of the conceptus with the maternal endometrium, followed by epithelial-mesenchymal transition (EMT) in trophoblast cells. These sequential events are necessary for establishing pregnancy in ruminants.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Biology
Kazuya Kusama, Rulan Bai, Yuta Matsuno, Atsushi Ideta, Toshihiro Sakurai, Kentaro Nagaoka, Masatoshi Hori, Kazuhiko Imakawa
Summary: This study identified candidate proteins, specifically SNX5, that could predict early pregnancy loss in heifers through metabolome and global proteome analyses.
Review
Cell Biology
Kazuhiko Imakawa, Kazuya Kusama, Tomoko Kaneko-Ishino, So Nakagawa, Koichi Kitao, Takayuki Miyazawa, Fumitoshi Ishino
Summary: The main roles of placentas include physical protection, nutrient and oxygen import, export of gases and fetal waste products, and endocrinological regulation. The differences in placental structures have not been explained or characterized, even though genes and gene control elements have been co-opted to explain the structural diversities.
Article
Veterinary Sciences
Yasuko Inagaki, Tomoko Kobayashi, Yoshihito Suda, Kazuya Kusama, Kazuhiko Imakawa
BMC VETERINARY RESEARCH
(2020)