Article
Agriculture, Multidisciplinary
Wenting An, Zhiqing Huang, Zhengyu Mao, Tianlei Qiao, Gang Jia, Hua Zhao, Guangmang Liu, Xiaoling Chen
Summary: This study found that dietary supplementation with taurine (TAU) can significantly improve meat quality, alter muscle fiber type, and enhance mitochondrial function in finishing pigs. These effects are achieved through the regulation of gene expression and activity, particularly through the CaN/NFATc1 pathway to promote muscle fiber-type conversion.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Sutian Wang, Cuiming Tang, Jianhao Li, Zhenjiang Wang, Fanming Meng, Guoqing Luo, Haiyun Xin, Jianwu Zhong, Yuan Wang, Baohong Li, Zhiyi Li, Lian Chen, Bin Hu, Sen Lin
Summary: This study found that feeding Tibetan pigs with mulberry leaf powder can improve meat quality, possibly due to enhanced antioxidant activity and altered muscle fiber type and morphology.
Article
Food Science & Technology
Sabine Conte, Candido Pomar, Diovani Paiano, Yan Duan, Pengfei Zhang, Janie Levesque, Frederic Guay, Nicolas Devillers, Luigi Faucitano
Summary: This study fed pigs with different feeding strategies but found no significant effects on pig growth and meat quality. The inefficiency of the dietary treatments applied may be attributed to the low ratio of fat to digestible carbohydrate in the diets and the mild pre-slaughter stress conditions encountered by the pigs.
Article
Agriculture, Dairy & Animal Science
Wanxue Wen, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Jun He, Yuheng Luo, Hui Yan, Hong Chen, Ping Zheng, Jie Yu
Summary: This study investigated the effects of dietary lycopene supplementation on meat quality, antioxidant capacity, and muscle fiber type transformation in finishing pigs. The results showed that adding 200 mg/kg lycopene to the diet improved the color, nutritional value, and juiciness of pork. It also enhanced the antioxidant capacity and promoted the conversion of muscle fiber types from fast-twitch to slow-twitch.
Article
Genetics & Heredity
Xiaofan Tan, Yu He, Yuqiao He, Zhiwei Yan, Jing Chen, Ruixue Zhao, Xin Sui, Lei Zhang, Xuehai Du, David M. M. Irwin, Shuyi Zhang, Bojiang Li
Summary: This study identified differentially expressed proteins in fast and slow muscles and constructed a protein-protein interaction network to elucidate the regulation of muscle fiber types by proteins.
Article
Agriculture, Multidisciplinary
Lingyu Zhang, Fengna Li, Qiuping Guo, Yehui Duan, Wenlong Wang, Yuhuan Yang, Yunju Yin, Saiming Gong, Mengmeng Han, Yulong Yin
Summary: This study investigated the effects of a balanced BCAA-supplemented protein-restricted diet on meat quality, muscle fiber types, and intramuscular fat (IMF) in finishing pigs. The results showed that this diet improved the growth performance, carcass weight, meat tenderness, and juiciness of the pigs. It also had positive effects on the composition of IMF and fatty acid profile, contributing to healthier meat.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Applied
Meng Xu, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Mingzhou Li, Jun He, Hong Chen, Ping Zheng, Jie Yu, Yuheng Luo, Bing Yu
Summary: The study found that dietary grape seed proanthocyanidin extract (GSPE) supplementation can improve meat quality, muscle fiber characteristics, and antioxidant capacity in finishing pigs. By increasing certain nutrient content and promoting specific protein expressions, GSPE enhances the overall nutritional value of pork.
Article
Food Science & Technology
Roberta Davoli, Jacopo Vegni, Alberto Cesarani, Corrado Dimauro, Martina Zappaterra, Paolo Zambonelli
Summary: This study found that genes related to mitochondrial functions, ATP biosynthesis, calcium homeostasis, and lipid metabolism were overexpressed in low glycolytic potential (GP) pork. This suggests that events related to postmortem meat quality may occur more rapidly in low GP muscles.
Article
Nutrition & Dietetics
Xin Zhang, Meixia Chen, Enfa Yan, Yubo Wang, Chenghong Ma, Pengguang Zhang, Jingdong Yin
Summary: The aim of this study was to investigate the effects of dietary malic acid supplementation on skeletal muscle fiber-type transition during the nursery period and subsequent meat quality in finishing pigs. The results showed that supplementation with malic acid for 28 days increased the percentage of oxidative fibers in weaned piglets, along with increased aerobic oxidation in serum and longissimus thoracis (LT) muscle. In addition, the activities of total antioxidant capacity and glutathione peroxidase in serum were increased. Furthermore, dietary malic acid supplementation during the nursery period tended to increase the pH(24h) and significantly decreased drip loss in the LT muscle of finishing pigs. The content of total saturated fatty acids (SFA) and total monounsaturated fatty acids in the LT muscle was significantly decreased, while the ratio of polyunsaturated fatty acids to SFA tended to increase. In conclusion, dietary malic acid supplementation during the nursery period can effectively increase antioxidant capacity and oxidative fibers' percentage in weaned piglets and improve the water holding capacity and nutritional values of pork in finishing pigs.
FRONTIERS IN NUTRITION
(2022)
Article
Agriculture, Dairy & Animal Science
Simeng Liao, Guang Liu, Bie Tan, Ming Qi, Xin Wu, Jianjun Li, Xiaoqing Li, Changfeng Zhu, Jiamei Huang, Shuo Zhang, Yulong Tang, Yulong Yin
Summary: This study investigates the biological effects of C60 on fattening pigs and demonstrates that C60 supplementation improves meat quality by regulating gene expression, lipid metabolism, and muscle fiber morphology.
Article
Food Science & Technology
Liping Cai, Yizhong Huang, Liepeng Zhong, Xiaoxiao Zou, Jiuxiu Ji, Xianxian Liu, Cong Huang, Qingjie Zeng, Bin Yang, Shijun Xiao, Junwu Ma, Lusheng Huang
Summary: Determining genetic correlations between replicated phenotypes across breeds or generations is crucial for animal breeding. In this study, we constructed a global eight-breeds crossbreed pig population and evaluated the genetic and phenotypic correlations of muscle fiber characteristics with meat quality traits. The results showed moderate to high genetic correlations between fiber density and various meat quality traits, providing important insights for improving meat quality through genetic regulation of muscle fiber characteristics.
Article
Agriculture, Dairy & Animal Science
A. Jiang, D. Yin, L. Zhang, B. Li, R. Li, X. Zhang, Z. Zhang, H. Liu, K. Kim, W. Wu
Summary: The study constructed miRNA expression profiles of porcine muscle fibers, identified highly expressed and differentially expressed miRNAs, and revealed their crucial role in regulating muscle fiber types and meat quality traits. This research laid a solid foundation for further exploring the regulatory mechanisms of miRNAs in livestock skeletal muscle fiber conversion and provided a genetic explanation for the role of miRNAs in human muscular diseases.
Article
Veterinary Sciences
Yongxiang Li, Yang He, Jinming Ran, Ying Huang, Xian Li, Hengxin Jiang, Xueyan Li, Yangsu Pan, Sumei Zhao, Chunlian Song, Hongbin Pan, Hong Hu
Summary: In order to promote the development and utilization of Saba pigs, the meat quality traits and glycolysis potential of three different crossbred pigs were compared. The results showed that the meat quality of BDS pigs was the best, indicating that the excellent characteristics of Saba pigs have been fully developed and utilized.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Gaoliang Bao, Xiu Liu, Jiqing Wang, Jiang Hu, Bingang Shi, Shaobin Li, Yuzhu Luo
Summary: The study investigated the effect of slaughter age on meat quality, fatty acid composition, and the expression of MyHC isoform genes in Tibetan sheep. Results showed differences in muscle fiber types at different ages, with 1.5-year-old Tibetan sheep having meat better suited for a healthy human diet. Additionally, meat quality and fatty acid composition of Tibetan sheep changed with increasing age, indicating the importance of age in regulating these characteristics.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Qian Li-li, Xie Jing-yi, Gao Ting, Cai Chun-bo, Jiang Sheng-wang, Bi Han-fang, Xie Shan-shan, Cui Wen-tao
Summary: Myostatin is a negative regulator of skeletal muscle growth and development. This study found that the skeletal muscle fiber types changed in MSTN-/- pigs, with an increase in lib type and a decrease in I and Ila types. The study also suggested that MSTN may regulate the directional development of muscle fiber types in the early stage of embryonic development.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Agriculture, Multidisciplinary
Lin-zhen Zhang, Li He, Ning Wang, Jia-hua An, Gen Zhang, Jin Chai, Yu-jie Wu, Chang-jiu Dai, Xiao-han Li, Ting Lian, Ming-zhou Li, Long Jin
Summary: This study identified a natural antisense transcript, APMAP-AS, which is associated with lipid synthesis and positively regulates adipogenic differentiation. APMAP-AS forms an RNA-RNA duplex with APMAP, increasing its stability and promoting lipid metabolism while inhibiting the expression of inflammatory factors.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Genetics & Heredity
Zhiming Wang, Jin Chai, Yuhao Wang, Yiren Gu, Keren Long, Mingzhou Li, Long Jin
Summary: Animal fat deposition has a significant impact on meat flavor and texture. In this study, we investigated the regulatory role of lncPLAAT3-AS in promoting preadipocyte proliferation and adipocyte differentiation. Our findings suggest that lncPLAAT3-AS upregulates PLAAT3 expression through a sponge-like mechanism involving miR-503-5p binding.
Article
Agriculture, Dairy & Animal Science
Hulong Lei, Qian Du, Naisheng Lu, Xueyuan Jiang, Mingzhou Li, Dong Xia, Keren Long
Summary: This study compared the effects of copper sulfate and copper glycinate on fecal microbiota and metabolites in pigs, and found that copper glycinate had more positive effects on gut health.
Article
Biochemistry & Molecular Biology
Xiaokai Li, Zhining Zhong, Ruowei Zhang, Jiaman Zhang, Yu Zhang, Sha Zeng, Qinjiao Du, Haoming Wang, Songling Zhang, Lu Lu, Mingzhou Li, Keren Long
Summary: In this study, a muscular dystrophy mouse model was created by Ptrf knockout, and single-nucleus RNA sequencing was used to investigate the transcriptional changes in skeletal muscle. The study revealed significant alterations in the transcriptome of different types of muscle fibers and mononuclear cells in response to muscular dystrophy, providing valuable insights into the molecular mechanisms of muscular dystrophy.
Article
Multidisciplinary Sciences
Long Jin, Danyang Wang, Jiaman Zhang, Pengliang Liu, Yujie Wang, Yu Lin, Can Liu, Ziyin Han, Keren Long, Diyan Li, Yu Jiang, Guisen Li, Yu Zhang, Jingyi Bai, Xiaokai Li, Jing Li, Lu Lu, Fanli Kong, Xun Wang, Hua Li, Zhiqing Huang, Jideng Ma, Xiaolan Fan, Linyuan Shen, Li Zhu, Yanzhi Jiang, Guoqing Tang, Bin Feng, Bo Zeng, Liangpeng Ge, Xuewei Li, Qianzi Tang, Zhihua Zhang, Mingzhou Li
Summary: Using a pig model, the study investigates how chromatin architecture in adipose tissue is regulated in relation to obesity. Analysis of the data reveals similarities and differences in chromatin architecture between humans and pigs, providing valuable insights into the regulation of obesity-related genes.
NATURE COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Yujie Wang, Xun Wang, Yi Luo, Jiaman Zhang, Yu Lin, Jie Wu, Bo Zeng, Lei Liu, Peiqi Yan, Jiyuan Liang, Hongrui Guo, Long Jin, Qianzi Tang, Keren Long, Mingzhou Li
Summary: This study generated a de novo pigeon genome assembly and conducted a high resolution spatio-temporal transcriptomic analysis of the crop epithelium in pigeons. The study identified "lactation"-related genes involved in lipid and protein metabolism and revealed extensive reorganization of promoter-enhancer interactions linked to the expression of these genes. The spatial localization of gene expression in specific epithelial layers was correlated with phenotypic changes in the crop.
Article
Biology
Qin Zou, Rong Yuan, Yu Zhang, Yifei Wang, Ting Zheng, Rui Shi, Mei Zhang, Yujing Li, Kaixin Fei, Ran Feng, Binyun Pan, Xinyue Zhang, Zhengyin Gong, Li Zhu, Guoqing Tang, Mingzhou Li, Xuewei Li, Yanzhi Jiang
Summary: Studying cellular specialization in different anatomical regions of pig skin can enhance our understanding of skin information and provide insights for human skin research.
Article
Geriatrics & Gerontology
Linyuan Shen, Tianci Liao, Qiuyang Chen, Yuhang Lei, Linghui Wang, Hao Gu, Yanhao Qiu, Ting Zheng, Yiting Yang, Chenggang Wei, Lei Chen, Ye Zhao, Lili Niu, Shunhua Zhang, Yan Zhu, Mingzhou Li, Jinyong Wang, Xuewei Li, Mailin Gan, Li Zhu
Summary: This study explores the role of tRNA-derived small RNAs (tsRNAs), specifically 5'tiRNA-Gly, in skeletal muscle regeneration. It is found that 5'tiRNA-Gly is significantly up-regulated after muscle injury and promotes inflammation through the TGF-beta signaling pathway. These findings provide a new potential target for skeletal muscle regeneration treatment.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Physiology
Jinwei Zhang, Yang Shen, Guitao Yang, Jing Sun, Chuang Tang, Hao Liang, Jideng Ma, Xiaoqian Wu, Haoran Cao, Meng Wu, Yuchun Ding, Mingzhou Li, Zuohua Liu, Liangpeng Ge
Summary: This study analyzed the impact of commensal microbiota on the host's mucosal immunity. It found that the absence of commensal microbiota led to smaller Peyer's patches in the gastrointestinal tract, particularly regarding B and T cells. Gene expression analysis revealed that commensal microbiota plays an important role in the development of mucosal immunity in the intestine. Overall, this study revealed the regulatory mechanisms of commensal microbiota in the host's Peyer's patches and emphasized the importance of early microbial colonization for mucosal immunity development.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Weiqiang Liu, Pingfen Zhu, Meng Li, Zihao Li, Yang Yu, Gaoming Liu, Juan Du, Xiao Wang, Jing Yang, Ran Tian, Inge Seim, Alaattin Kaya, Mingzhou Li, Ming Li, Vadim N. Gladyshev, Xuming Zhou
Summary: The variation in lifespan among mammals is significant, and the study aims to uncover the evolutionary forces and molecular features related to longevity. Comparative transcriptomics analysis of liver, kidney, and brain tissues of 103 mammalian species revealed that only a few genes have common expression patterns with longevity. However, pathways related to translation fidelity and methionine restriction showed correlation with lifespan. The study suggests that lifespan regulation through gene expression is driven by polygenic and indirect natural selection.
Article
Biochemistry & Molecular Biology
Jingyi Bai, Yu Lin, Jiaman Zhang, Ziyu Chen, Yujie Wang, Mingzhou Li, Jing Li
Summary: The study used ATAC-seq and rRNA-depleted RNA-seq to analyze the chromatin accessibility and transcriptional features in multiple tissues of newborn pigs and during porcine liver development. The researchers obtained a comprehensive annotation of consensus ATAC-seq peaks for each tissue and developmental stage, and found that tissue-specific accessible promoters were associated with active transcription and tissue-specific functions. Additionally, they observed a close association between dynamic chromatin accessibility and hepatic function transition during liver postnatal development. This study expands our understanding of epigenetic regulation in mammalian tissues and organ development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jing Yang, Xingxing Zhu, Rui Wang, Mingzhou Li, Qianzi Tang
Summary: In this study, we comprehensively evaluated the performance of 24 popular state-of-the-art methods for complete end-to-end Hi-C data analysis using manually curated or experimentally validated benchmark datasets. Our results indicate that HiC-Pro, DomainCaller, and Fit-Hi-C2 showed relatively balanced performances in different aspects, providing a reference for researchers to choose suitable Hi-C analysis tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Huan Liu, Dengke Pan, Pu Li, Dandan Wang, Bo Xia, Ruixin Zhang, Junfeng Lu, Xiangyang Xing, Jiaxiang Du, Xiao Zhang, Long Jin, Lin Jiang, Linong Yao, Mingzhou Li, Jiangwei Wu
Summary: A novel sepsis pig model is created by modifying the surgical strategy of cecal ligation and puncture (CLP), which for the first time recapitulates the whole course of sepsis in humans. Increased levels of ZBED6 in skeletal muscle are shown in this model and sepsis patients, and protection against sepsis-induced muscle wasting is demonstrated in ZBED6-deficient pigs. The direct target of ZBED6 is revealed to be DOCK3, and septic ZBED6-deficient pigs show increased DOCK3 expression in skeletal muscle and myocytes, activating the RAC1/PI3K/AKT pathway and protecting against sepsis-induced muscle wasting.
Article
Multidisciplinary Sciences
Pengliang Liu, Diyan Li, Jiaman Zhang, Mengnan He, Yan Li, Rui Liu, Mingzhou Li
Summary: The storage of lipids as energy in adipose tissue is conserved, but there are substantial differences in the physiological activities of adipose tissues among species. This study characterized the transcriptional and lipidomic profiles of subcutaneous and visceral adipose tissues across 15 vertebrate species, spanning more than 300 million years of evolution. The data revealed greater variation in AT transcriptome and lipidome among species than within the same species, providing a resource for future studies on functional differences among adipose tissues in vertebrate species.
Article
Biochemistry & Molecular Biology
Jing Li, Teng Yuan, Anjing Zhang, Peidong Yang, Li He, Keren Long, Chuang Tang, Li Chen, Mingzhou Li, Lu Lu
Summary: Macrophages are essential for immunity and play important roles in tissue homeostasis and metabolism. There is currently no standardized method for isolating and differentiating porcine macrophages. This study identified distinct M1 and M2 macrophage subsets in pigs and compared their gene expression profiles. The results showed that porcine macrophages have similar gene signatures to human and mouse macrophages. The study also demonstrated the potential prognostic value of macrophage signatures in differentiating pathogen infections.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
