期刊
GENETICS AND MOLECULAR RESEARCH
卷 14, 期 3, 页码 8829-8838出版社
FUNPEC-EDITORA
DOI: 10.4238/2015.August.3.6
关键词
Gypsophila; Random amplification of polymorphic DNA; Genetic diversity; ISSR
资金
- TUBITAK [HD 105T299, 105T147, EUBAP-2011-10.01.05]
Gypsophila L. is the 3rd-largest genus of Caryophyllaceae in Turkey, a country that includes 60 taxa belonging to 56 species of the 126 recognized in the genus. A total of 35 taxa are endemic to Turkey, with an approximately 60% endemism ratio. In this study, the genetic diversity of 14 Gypsophila species from Turkey was analyzed using random amplification of polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Sixteen RAPD and 6 ISSR primers produced 132 polymorphic bands. RAPD, ISSR, and RAPD + ISSR primers for the 14 species showed 92.7, 93.8, and 92.9% polymorphism, respectively. Our results indicate that RAPD and ISSR markers are reliable and effective for assessing the genetic diversity of Gypsophila species. Gypsophila species studied were separated into 2 clusters. Our analysis shows that the clusters correlated with geographic and phytogeographic regions.
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