期刊
JOURNAL OF AEROSOL SCIENCE
卷 124, 期 -, 页码 54-67出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jaerosci.2018.07.004
关键词
Electrostatic precipitator; Personal sampler; Wire-to-Wire charger; Collection efficiency; Biological efficiency
类别
资金
- Personal Electrostatic Bioaerosol Sampler (PEBS) with High Sampling Flow Rate [R2110560]
- Design of Advanced Electrostatic Sampler for Total Bioaerosols from CDC-NIOSH [R01OH009783]
We recently developed a new personal electrostatic bioaerosol sampler (PEBS) for determining exposures to airborne microorganisms. PEBS was shown to collect airborne non-biological particles with efficiencies approaching 80% while producing very low ozone concentrations. In this work, we analyzed the performance of this sampler when collecting airborne Bacillus atrophaeus bacterial cells and Penicillium chrysogenum fungal spores as a function of sampling flow rates (e.g., 10 and 20 L/min) and sampling time (e.g., 10, 60, and 240 min). The collected samples were analyzed using microscopy, adenosine triphosphate (ATP)-based bioluminescence, flow cytomerry (Live/Dead test), and culture techniques. PEBS's physical and biological performance was compared against that of BioSampler (SKC Inc., Eighty Four, PA) when the samplers were operated at 10 and 12.5 L/min, respectively. PEBS achieved physical collection efficiency as high as 83%, and its physical performance in terms of measured bioaerosol concentration was better than that of BioSampler. In addition, a fraction of live microorganisms recovered by PEBS was not different from that of BioSampler. Compared to BioSampler, PEBS measured similar or higher concentrations of culturable bacteria, but lower concentrations of culturable spores. The airborne ATP concentration measured by PEBS was significantly higher than that measured by BioSampler. Overall, we show that PEBS is a viable and efficient technology to determine personal exposures to airborne microorganisms using multiple sample analysis techniques.
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