期刊
JOURNAL DE MYCOLOGIE MEDICALE
卷 28, 期 2, 页码 320-326出版社
MASSON EDITEUR
DOI: 10.1016/j.mycmed.2018.03.003
关键词
Candida albicans; Candida glabrata; Typing method; Rep-PCR; DiversiLab (R); MultiLocus sequence typing; MultiLocus microsatellite typing
类别
资金
- Nimes University Hospital
The objective of our study was to assess the DiversiLab (R) automated repetitive sequence-based PCR (rep-PCR) system for typing C. albicans and C. glabrata isolates and to compare it with two proven and routinely used typing methods. A total of 39 isolates from 11 patients with candidaemia or tissue candidiasis (two to six isolates per patient) were analyzed with three typing methods: DiversiLab (R) rep-PCR, multilocus sequence typing and multilocus microsatellite typing. DiversiLab (R) rep-PCR results were consistent with those obtained using the two other typing methods for C. albicans, but not for C. glabrata. Thanks to its simplicity of use, rapidity, standardization and reproducibility, the DiversiLab (R) rep-PCR system is an interesting tool to investigate C. albicans infections. (C) 2018 Elsevier Masson SAS. All rights reserved.
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