期刊
GENETICS
卷 200, 期 1, 页码 123-U269出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.115.176172
关键词
Caenorhabditis elegans; ASJ neuron; promoter; thioredoxin; ASJ motif; SPTF-1 transcription factor
资金
- National Institutes of Health Office of Research Infrastructure Programs [P40 OD010440]
- Japanese National Bioresource Project
- Japanese Ministry of Education, Culture, Sport, Science and Technology
- Junta de Andalucia [P07-CVI-02697, P08-CVI-03629]
- Swedish Research Council
- Torsten Soderberg Foundation
- European Union FP6 Marie Curie Research Training Network EUrythron [MRTN-CT-2004-005499]
The identity of a given cell type is determined by the expression of a set of genes sharing common cis-regulatory motifs and being regulated by shared transcription factors. Here, we identify cis and trans regulatory elements that drive gene expression in the bilateral sensory neuron ASJ, located in the head of the nematode Caenorhabditis elegans. For this purpose, we have dissected the promoters of the only two genes so far reported to be exclusively expressed in ASJ, trx-1 and ssu-1. We hereby identify the ASJ motif, a functional cis-regulatory bipartite promoter region composed of two individual 6 bp elements separated by a 3 bp linker. The first element is a 6 bp CG-rich sequence that presumably binds the Sp family member zinc-finger transcription factor SPTF-1. Interestingly, within the C. elegans nervous system SPTF-1 is also found to be expressed only in ASJ neurons where it regulates expression of other genes in these neurons and ASJ cell fate. The second element of the bipartite motif is a 6 bp AT-rich sequence that is predicted to potentially bind a transcription factor of the homeobox family. Together, our findings identify a specific promoter signature and SPTF-1 as a transcription factor that functions as a terminal selector gene to regulate gene expression in C. elegans ASJ sensory neurons.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据