Pseudooceanicola lipolyticus sp nov., a marine alphaproteobacterium, reclassification of Oceanicola flagellatus as Pseudooceanicola flagellatus comb. nov and emended description of the genus Pseudooceanicola

A Gram-stain-negative, rod-shaped bacterium, designated 157(T), was isolated from seawater collected from the Philippine Sea. Cells of strain 157(T) grew in medium containing 0.5-10.0% NaCl (w/v, optimum 3 %), at pH 6.0-8.5 (optimum 7.0) and at 15-40 degrees C (optimum 30 degrees C). Tweens 20, 40 and 80 as well as urea were hydrolysed. The 16S rRNA gene sequence of strain 157(T) had a high sequence similarity with respect to Pseudooceanicola marinus AZO-C-T (97.2 %), and exhibited less than 97.0% sequence similarity to other type strains of the species with validly published names. Phylogenetic analyses revealed that strain 157(T) fell within a cluster comprising the Pseudooceanicola species and formed a coherent clade with P. marinus AZO-C-T and Pseudooceanicola antarcticus Ar-45(T). Strain 157(T) exhibited average nucleotide identity values of 74.5 and 74.9% to P. marinus LMG 23705(T) and P. antarcticus Ar-45(T), respectively. In silico DNA-DNA hybridization analysis revealed that strain 157(T) shared 20.2% DNA relatedness with P. marinus LMG 23705(T) and 20.6% with P. antarcticus Ar-45(T), respectively. The sole isoprenoid quinone was ubiquinone 10. The major fatty acids were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), C-19:0 cyclo omega 8c, C-16:0 2-OH and C-16:0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content was 64.6 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus Pseudooceanicola, for which the name Pseudooceanicola lipolyticus is proposed. The type strain is 157(T) (=KCTC 52654(T)=MCCC 1K03317(T)). In addition, the description of the genus Pseudooceanicola is emended and Oceanicola flagellatus is reclassified as Pseudooceanicola flagellatus comb. nov., with the type strain DY470(T) (=CGMCC 1.12664(T)=LMG 27871(T)) proposed.