Polaribacter staleyi sp nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis

A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139(T), was isolated from the Pacific red alga Ahnfeltiato buchiensis. The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938(T) (99.3 %) and 93.3-98.6% to other recognized Polaribacter species. The prevalent fatty acids of strain 10Alg 139(T) were iso-C-15 : 0 3-OH, C-15 (: 0) 3-OH, iso-C-15: 0, iso-C-13 (: 0), C-15 : 0 and C-15 : 1 omega 6c. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids. The main respiratory quinone was menaquinone 6. The DNA G+C content of the type strain is 31.8 mol%. The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacter staleyi sp. nov. is proposed. The type strain is 10Alg 139(T) (=KCTC 52773(T)=KMM 6729(T)).