Pinisolibacter ravus gen. nov., sp nov., isolated from pine forest soil and allocation of the genera Ancalomicrobium and Pinisolibacter to the family Ancalomicrobiaceae fam. nov., and emendation of the genus Ancalomicrobium Staley 1968

A novel bacterium, designated strain E9(T), was isolated from pine forest soil of Kyonggi University (Suwon, Republic of Korea). Cells were facultatively anaerobic, Gram-staining-negative, catalase-negative, oxidase-positive, non-motile, non-sporeforming, rod-shaped and straw coloured. Prosthecae were absent. Glucose was fermented. The strain grew in the pH range of 5.0-10.0 (optimum, 6.5-8.5) and at 45 degrees C (optimum, 28-32 degrees C). E9(T) was sensitive to NaCl at low concentration and tolerated only 0.2 % NaCl (w/v). A phylogenetic analysis based on 16S rRNA gene sequences revealed that E9(T) formed a lineage within the phylum Proteobacteria that was distinct from various members of the order Rhizobiales, including Ancalomicrobium adetum DSM 4722(T) (94.76 % sequence similarity), 'Nitratireductor lucknowense' IITR-21 (92.72 %), Prosthecomicrobium hirschii 16(T)(92.66 %) and Kaistia soli DSM 19436(T) (92.53 %). The predominant isoprenoid quinone was Q-10. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl-Nmethylethanolamine. Major cellular fatty acids were summed feature 8 (C-10:1 omega 7c and/or C-10:1 omega 6c), summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), and C-16:0. The DNA G+C content of the type strain was 68.4 mol%. Polyphasic characterization indicated that strain E9(T) represented a novel species in a novel genus within a novel family, for which the name Pinisolibacter ravus gen. nov., sp. nov. is proposed. The type strain of Pinisolibacter ravus is E9(T) (=KEMB 9005-534(T) =KACC 19120(T) =NBRC 112686(T)). A formal allocation of the genus Ancalomicrobium to the family Ancalomicrobiaceae fam. nov. is also proposed.