Article
Biochemistry & Molecular Biology
Yoshie Haratake, Kohei Sano, Miki Tsuchiya, Kaori Minaki, Masayuki Munekane, Toshihide Yamasaki, Masayori Hagimori, Takahiro Mukai
Summary: This study designed and synthesized a novel radiolabeled amyloid imaging probe, demonstrating its potential for diagnosing amyloidosis.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Sadia Malik, Faisal Nabi, Mohammad Khursheed Siddiqi, Aiman Masroor, Malik Hisamuddin, Tajalli Ilm Chandel, Nabeela Majid, Rizwan Hasan Khan
Summary: This study confirms the inhibitory effect of lipophilic FAEE on insulin fibrillation, which can cross the blood brain barrier and stabilize the monomeric state of insulin, thereby preventing the extension of fibrillation.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Physical
Kateryna Vus, Uliana Tarabara, Ihor Danylenko, Yaroslav Pirko, Tetiana Krupodorova, Alla Yemets, Yaroslav Blume, Vitalii Turchenko, Dmytro Klymchuk, Petro Smertenko, Olga Zhytniakivska, Valeriya Trusova, Sergii Petrushenko, Sergey Bogatyrenko, Galyna Gorbenko
Summary: Silver nanoparticles (SNPs) synthesized extracellularly using extracts of different genus Pleurotus fungi samples showed pronounced anti-amyloid effect in vitro, suggesting their potential application as effective anti-amyloid agents.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Food Science & Technology
Amir Vahedifar, Jianping Wu
Summary: This paper provides a critical review of food peptide-based self-assembles and the SAPs involved in their structure, as well as discussing their potential applications in the food industry.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Susanna Navarro, Marta Diaz-Caballero, Francesca Peccati, Lorena Roldan-Martin, Mariona Sodupe, Salvador Ventura
Summary: Enzymes fold into specific 3D protein structures and exhibit high catalytic efficiency and selectivity. Artificial amyloids have been shown to have catalytic activity and advantages over natural enzymes. A recent study found that short peptides can self-assemble into amyloid fibrils with catalytic activity by coordinating and retaining different divalent metal cations. These findings provide new insights into the design of artificial metalloenzymes and support the role of amyloid-like structures in the origin of life.
Article
Chemistry, Analytical
Katarina Siposova, Dagmar Sedlakova, Andrey Musatov
Summary: Monitoring the surface tension values of insulin is a valuable tool for detecting its amyloid aggregation, as the aggregation leads to an increase in surface tension values. The kinetics of fibril formation and fibril morphology can be validated by ThT fluorescence and AFM, respectively.
ANALYTICAL METHODS
(2021)
Article
Engineering, Biomedical
Shuhui Yang, Jinjin Zhu, Changfeng Lu, Yi Chai, Zheng Cao, Jiaju Lu, Zhe Zhang, He Zhao, Yin-Yuan Huang, Shenglian Yao, Xiangdong Kong, Peixun Zhang, Xiumei Wang
Summary: The AFG/fSAP hydrogel, with synergistic topographical and biochemical cues, successfully repaired a 15-mm peripheral nerve defect in rats through enhancing alignment and neurotrophin secretion. It showed satisfactory morphological and functional recovery, comparable to autografts, and activated regeneration-associated gene expression and signaling pathways.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Maria Gessica Ciulla, Raffaele Pugliese, Fabrizio Gelain
Summary: This study made significant advancements in the field of chemical cross-linked self-assembling peptides (SAPs), bringing them closer to the goal of stiff peptidic materials suitable for the regeneration of various tissues, such as skin, muscle, and lung.
Article
Biochemistry & Molecular Biology
Xinyue Lan, Longjiao Zhu, Yangzi Zhang, Keren Chen, Jia Wang, Zaihui Du, Shuting Li, Xu Chen, Wentao Xu
Summary: This study proposes a new form of Thioflavin T nucleic acid switches called CHTLNAS, which have excellent fluorescence excitation properties and can achieve label-free and enzyme-free detection of tetracycline and berberine within 10 minutes at the pM level. It can also track intracellular carriers and prolong the nuclear-entry time of Thioflavin T, expanding its application in cell biology research.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Physical
Olga Zhytniakivska, Atanas Kurutos, Mykhailo Shchuka, Kateryna Vus, Uliana Tarabara, Valeriya Trusova, Galyna Gorbenko
Summary: This study demonstrates that the Fo spacing diaeresis rster resonance energy transfer (FRET) technique can differentiate between the non-fibrillized and fibrillar states of insulin. The presence of cyanine dyes causes a significant decrease in ThT fluorescence, with the magnitude of this effect depending on the cyanine chemical structure.
CHEMICAL PHYSICS LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Paula Mayorga-Burrezo, Carmen C. Mayorga-Martinez, Martin Pumera
Summary: Microrobots are seen as attractive mobile carriers for clinical therapies, with high expectations for their ability to navigate autonomously and perform specific tasks. In this study, self-propelled light-driven single-component micromotors based on concave BiVO4 microspheres are used to disaggregate protein fibrils by generating reactive oxygen species (ROS) on-the-fly. This promising application could potentially be extended to photodynamic therapies for conditions such as lung or skin cancer.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Jesus Camara-Almiron, Laura Dominguez-Garcia, Nadia El Mammeri, Alons Lends, Birgit Habenstein, Antonio de Vicente, Antoine Loquet, Diego Romero
Summary: This study demonstrates that recombinantly purified RcTasA retains the biochemical properties of the entire protein and shows that two aggregation-prone stretches and imperfect amino acid repeats in RcTasA contribute to its amyloid-like capacity. The study also identifies the critical role of residues D64, K68, and D69 in the structural function of TasA. Experiments with mutant versions of TasA reveal a partial loss of function in the assembly of the extracellular matrix or the stability of the core and amyloid-like properties. Overall, these findings provide insights into the polymerization process of TasA and the sequence determinants that govern the behavior of protein filaments in bacteria.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Biochemistry & Molecular Biology
Anna Sulatskaya, Georgy N. Rychkov, Maksim Sulatsky, Ekaterina Mikhailova, Nadezhda M. Melnikova, Veronika S. Andozhskaya, Irina M. Kuznetsova, Konstantin K. Turoverov
Summary: This comprehensive study provides new evidence on the amyloid polymorphism of Aβ 40 and Aβ 42. It shows that Aβ 42 fibers have a higher tendency to form large clots and exhibit stronger resistance to destabilizing effects, as well as explicit cytotoxicity. In contrast, Aβ 40 fibers have a lower tendency to cluster and weaker resistance to external factors. Additionally, cross-seeding of Aβ 40 fibrillogenesis using preformed Aβ 42 fibrils changes the morphology and increases the stability and cytotoxicity of Aβ 40 fibrils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Georgia I. Nasi, Foteini D. Aktypi, Panagiotis M. Spatharas, Nikolaos N. Louros, Paraskevi L. Tsiolaki, Vassiliki Magafa, Ioannis P. Trougakos, Vassiliki A. Iconomidou
Summary: This study investigates the amyloidogenic properties of a 34-residue-long peptide in Arabidopsis thaliana under different pH conditions, and explores its association with other A. thaliana proteins using bioinformatics tools.
Article
Chemistry, Multidisciplinary
Meijun Li, Minghua Liu, Yinlin Sha
Summary: The study demonstrates that the co-assembly of Thioflavin T (ThT) with T1 amyloid fibrils can lead to chiroptical inversion, indicating that the morphological helicity of template fibrils cannot be distinguished solely by the sign of chiroptical spectra of the dye/peptide assemblies.
Article
Toxicology
YiNan Zhang, YuJun Zhang, ChaoYi Deng, DeYing Gong, Yi Kang, Jin Liu, WenSheng Zhang
JOURNAL OF APPLIED TOXICOLOGY
(2020)
Article
Anesthesiology
Cheng Zhou, Lei Tang, Qinqin Yin, Linghui Yang, Deying Gong, Yi Kang, Hangxue Cao, Jing Fan, Yujun Zhang, Duo Qian, Qianqian Zhang, Bowen Ke, Jin Liu, Wensheng Zhang, Jun Yang
REGIONAL ANESTHESIA AND PAIN MEDICINE
(2020)
Article
Multidisciplinary Sciences
YuJun Zhang, YingYing Jiang, HaiYan Wang, Bin Wang, Jun Yang, Yi Kang, Jun Chen, Jin Liu, Wen-sheng Zhang
Article
Chemistry, Multidisciplinary
Yongzhu Chen, Fei Peng, Tao Su, Hao Yang, Feng Qiu
Review
Chemistry, Medicinal
Qi Wang, Yujun Zhang, Jin Liu, Wensheng Zhang
Summary: Local anesthetics provide complete analgesia with fewer side effects compared to systemic analgesics, but their clinical use is limited by their short duration of action. Therefore, local anesthetics with fast onset, long duration, selective nociceptive block, and low toxicity are highly desirable. Quaternary lidocaine derivatives (QLDs) have shown these characteristics in past electrophysiological studies.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Toxicology
Peiwen Gao, Yujun Zhang, Chaoyi Deng, Deying Gong, Yi Kang, Jin Liu, Wensheng Zhang
Summary: The study evaluated the potential toxicity of QXOH-LB in NIH mice, with 10 mg/kg QXOH-LB and 5 mg/kg QXOH found to be well tolerated without toxicity symptoms. The NOAEL was considered to be 10 and 5 mg/kg/day for QXOH-LB and QXOH, respectively.
JOURNAL OF APPLIED TOXICOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zhihua Xing, Yongzhu Chen, Feng Qiu
Summary: The traditional belief that beta-strand conformation is necessary for peptide fibrillization has been challenged in this study, where a peptide fibrillization pathway starting from a disordered structure was observed. Peptides could still form nanofibers via intermolecular assembly at low concentrations, and transition into beta-strand conformation at higher concentrations. This suggests that factors like hydrophobic and electrostatic interactions can drive peptide self-assembly even without ordered beta-strand conformation.
Article
Nanoscience & Nanotechnology
Jing Liu, Fei Peng, Yi Kang, Deying Gong, Jing Fan, Wensheng Zhang, Feng Qiu
Summary: A peptide GQY was designed as an alternative to typical hydrophobic amino acids for self-assembling peptides, showing higher efficiency in encapsulating hydrophobic drugs. Stable nanoparticles with plenty of hydrophobic cavities could be formed by GQY, encapsulating hydrophobic drugs at high concentration. GQY exhibited biocompatibility and reduced pain-on-injection as a carrier for hydrophobic drugs, presenting a promising option for drug delivery systems.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Physical
Fei Peng, Yongzhu Chen, Jing Liu, Zhihua Xing, Jing Fan, Wensheng Zhang, Feng Qiu
Summary: A simple strategy was proposed to design gemini surfactant-like peptides, and a peptide named APK was successfully designed with strong self-assembly and hydrophobic encapsulation capabilities. Furthermore, APK showed potential for anti-tumor, anesthetic, and antimicrobial applications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Engineering, Biomedical
Fei Peng, Jing Liu, Yujun Zhang, Jing Fan, Deying Gong, Liu He, Wensheng Zhang, Feng Qiu
Summary: This study proposes a biomineralization strategy based on self-assembling peptides for the slow release of water-soluble small molecular drugs such as lidocaine. By inducing the biomineralization of lidocaine through pH adjustment, uniform microcrystals were formed and incorporated into a hyaluronic acid hydrogel to prepare an injectable formulation. The formulation demonstrated slow drug release and generated a prolonged effect in animal models.
ACTA BIOMATERIALIA
(2022)
Article
Pharmacology & Pharmacy
Ying Ying Jiang, Yu Jun Zhang, Zhao Qiong Zhu, Yi Dan Huang, Da Chun Zhou, Jing Chen Liu, Chao Yu Li, Jin Liu, Bin Liu, Wen Sheng Zhang
Summary: This study investigated the effectiveness, safety, and pharmacokinetics of Adamgammadex in surgical patients. The results showed that Adamgammadex effectively reversed the neuromuscular block induced by rocuronium, and it was safe and well tolerated in patients.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Fei Peng, Jing Liu, Yujun Zhang, Guoyan Zhao, Deying Gong, Liu He, Wensheng Zhang, Feng Qiu
Summary: A new long-acting ropivacaine nanocrystal formulation was developed through the interaction between a peptide and ropivacaine, allowing for controllable biomineralization and extended anesthetic and analgesic effects without toxicity in rodent models. This crystallization strategy utilizing the peptide-drug interaction provides a promising pathway for fabricating long-acting formulations for various small molecular drugs.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Yongzhu Chen, Feng Qiu, Chengkang Tang, Zhihua Xing, Xiaojun Zhao
Summary: Flexible long nanofibers designed in the study demonstrated various self-patterning behaviors on a specific surface, and different fiber alignment patterns could be obtained by adjusting the incubation conditions.
NANOSCALE ADVANCES
(2021)
Article
Toxicology
Jie Zhang, YuJun Zhang, Qinqin Yin, Jun Yang, Yi Kang, Deying Gong, Jin Liu, WenSheng Zhang
Summary: QXOH-LB showed dose-dependent toxicity in beagle dogs, with adverse effects that resolved within 48 hours. The no-observed-adverse-effect level for QXOH and QXOH-LB was determined to be 2 mg/kg. Toxicokinetic data indicated increased exposure to QXOH and LB with higher doses of QXOH-LB, with no evidence of drug accumulation or gender-related effects.
JOURNAL OF APPLIED TOXICOLOGY
(2021)