期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 41, 期 4, 页码 2279-2287出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2018.3404
关键词
NALP3; NALP3 inflammasome; pulmonary fibrosis; NF-B pathway; fibroblast
资金
- Sichuan Provincial Department of Science and Technology [0040205301A53]
The NALP3 inflammasome interacts with various immune and cell metabolic pathways and may participate in pulmonary fibrosis. However, little is known on its regulatory mechanism with respect to collagen synthesis. The objective of the present study was to investigate whether NALP3 inflammasome activation is involved in H2O2-mediated collagen synthesis, in addition to examining the possible cell signaling mechanisms underlying this effect. It was demonstrated that the NF-B signaling pathway was activated under conditions of H2O2-mediated oxidative stress in NIH-3T3 mouse embryonic fibroblasts. H2O2-exposed fibroblasts exhibited activated NALP3 inflammasomes via increased NALP3, apoptosis-associated Speck-like protein and caspase-1 expression and the secretion of interleukin-1. H2O2 also elevated -SMA and type I collagen expression. In vitro silencing of NALP3 attenuated the degradation of IB and decreased the synthesis of type I collagen. Furthermore, the NALP3 inflammasome was found to be activated in bleomycin-induced pulmonary fibrosis in mice, and this activation was relieved by a nuclear factor (NF)-B inhibitor. Taken together, these findings indicate that the NALP3 inflammasome is involved in H2O2-induced type I collagen synthesis, which is mediated by the NF-B signaling pathway. Additionally, the NALP3 inflammasome contributes to the development of bleomycin-induced pulmonary fibrosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据