期刊
GENES & DEVELOPMENT
卷 29, 期 21, 页码 2225-2230出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.267708.115
关键词
differentiation; epidermis; transcription
资金
- U.S. Department of Veterans Affairs Office of Research and Development
- National Institute of Arthritis and Musculoskeletal and Skin Diseases National Institutes of Health [R01 AR45192, F32 AR063508]
Outward migration of epidermal progenitors occurs with induction of hundreds of differentiation genes, but the identities of all regulators required for this process are unknown. Weused laser capture microdissection followed by RNA sequencing to identify calmodulin-like 5 (CALML5) as the most enriched gene in differentiating outer epidermis. CALML5 mRNA was up-regulated by the ZNF750 transcription factor and then stabilized by the long noncoding RNA TINCR. CALML5 knockout impaired differentiation, abolished keratohyalin granules, and disrupted epidermal barrier function. Mass spectrometry identified SFN (stratifin/14-3-3s) as a CALML5-binding protein. CALML5 interacts with SFN in suprabasal epidermis, cocontrols 13% of late differentiation genes, and modulates interaction of SFN to some of its binding partners. AZNF750-TINCR-CALML5-SFN network is thus essential for epidermal differentiation.
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