Article
Biochemistry & Molecular Biology
Shuai Zhang, Lang Xie, Shuqing Zheng, Baoyue Lu, Wenjing Tao, Xiaoshuang Wang, Thomas D. Kocher, Linyan Zhou, Deshou Wang
Summary: This study conducted genome-wide identification and comprehensive analysis of the SDR superfamily in 29 animal species, revealing that the number of SDR genes in animals increased with whole genome duplication, and vertebrate SDRs were clustered into five categories. Tandem duplication of specific SDR genes was observed in different animal groups, and sexually dimorphic expression of certain SDRs in adult fish gonads was confirmed by in situ hybridization and qPCR, suggesting their potential roles in steroidogenesis and gonadal differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jianshuang Li, Min Song, Haishen Wen, Ying Zhang, Yun Li, Likang Lyu, Xiaojie Wang, Xin Qi
Summary: Using black rockfish as a research model, this study investigated the gonad lipidomics and gene expression levels during different developmental stages, revealing a potential regulatory network of lipid metabolism. Through UHPLC-QTOFMS, four significant differential glycerophospholipid metabolic pathways were identified, and pathway-related enzyme-coding genes were confirmed and analyzed. The findings provide insights into substance metabolism and regulation during gonad development in ovoviviparity teleosts.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Sook-Kyoung Heo, Ho-Min Yu, Do Kyoung Kim, Hye Jin Seo, Yerang Shin, Sung Ah Kim, Minhui Kim, Youjin Kim, Yoo Jin Lee, Eui-Kyu Noh, Jae-Cheol Jo
Summary: Liver transplantation is the most effective treatment option for patients with liver failure, but there are limitations such as donor shortage, complications, high costs, and the need for immunosuppressive therapy. An alternative approach is liver cell transplantation. This study investigated the effect of a protein called LIGHT on the differentiation of human bone marrow-derived stem cells into liver-like cells.
Article
Biochemistry & Molecular Biology
Zhigang Yang, Hangbo Zhu, Xiaoping Huang, Aimin Wang, Dizhi Xie
Summary: This study investigated the characteristics of the acsl gene family in golden pompano and found that acsl1 and acsl3 were widely expressed in multiple tissues, while acsl4, acsl5, and acsl6 were mainly expressed in the brain. The study also showed that the expression levels of different acsl genes and the fatty acid content in the muscle varied depending on the type of dietary fatty acids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiaoming Bai, Changle Zhao, Ximei Qu, Zeming Zhang, Wenjing Tao, Linyan Zhou, Deshou Wang, Jing Wei
Summary: Nanog, a core transcription factor in pluripotency maintenance network, shows distinct expression patterns in fishes compared to mammals. The identification and study of Nile tilapia nanog (Ong) revealed unique spatiotemporal expression patterns during both embryonic and gonadal development. This study challenges the conservative assumption of Nanog expression in fish species and provides valuable insights for further functional and evolutionary research of this gene.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Oncology
Maria Cecilia Lira, Francisco D. D. Rosa, Ignacio Aiello, Mileni Soares Machado, Alejandra G. G. Palma, Leonardo Paz, Maria Cecilia Salazar Guemes, Silvia Burlando, Pablo J. J. Azurmendi, Natalia Paladino, Monica A. Costas, Maria Fernanda Rubio
Summary: Nuclear receptor coactivator 3 (NCoA3) is amplified or overexpressed in several types of cancer, including breast tumors. This study investigated the modulation of NCoA3 in breast cancer-associated adipocytes and its association with the expression of inflammatory markers.
Article
Endocrinology & Metabolism
Matthew K. H. Wong, Mulan He, Kong Hung Sze, Tao Huang, Wendy K. W. Ko, Zhao-Xiang Bian, Anderson O. L. Wong
Summary: Spexin, a highly conserved neuropeptide, is associated with various pathological conditions. Studies on mouse SPX revealed its solution structure, binding mechanism with receptors GalR2/3, and ubiquitous tissue distribution.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zuliang Qin, Zhiqiang Li, Shuangyi Yang, Feilong Wang, Tian Gao, Wenjing Tao, Linyan Zhou, Deshou Wang, Lina Sun
Summary: Histone lysine demethylases (KDM) are responsible for histone demethylation and play important roles in gonadal development of vertebrates. The KDM gene family originated from early diverging metazoans and significantly expanded in vertebrates with multiple whole genome duplication events, particularly during third-round whole genome duplication and polyploidization of teleosts.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Punyamanee Yamkate, Randi M. Gold, David C. Twedt, Jan S. Suchodolski, Joerg M. Steiner, Jonathan A. Lidbury
Summary: This study investigated the intracellular distribution of copper in the liver of cats, and found that copper is mainly distributed in the cytosolic and nuclear fractions.
Article
Fisheries
Min Cao, Ningning Wang, Xu Yan, Ning Yang, Qiang Fu, Xiaoyan Zhang, Yong Zhang, Chao Li
Summary: This study identified TNFSF genes and their receptors in S. schlegelii through a genome-wide survey. The study found that these genes experienced duplication and whole genome duplication events, and performed clustering analysis through molecular phylogenetic analysis. Protein domain and motif analysis revealed the structural features of TNFSF and TNFRSF. Synteny analysis showed significant changes in neighboring genes of TNFSF and TNFRSF compared to other species. Additionally, qRT-PCR and protein-protein interaction network analysis demonstrated differential expression of these genes after infection. This study provides new insights into the characterization and evolution of TNFSF genes and their receptors in S. schlegelii.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Entomology
Zhenchen Wu, Jia Ye, Jiali Qian, Endang Rinawati Purba, Qinghe Zhang, Longwa Zhang, Dingze Mang
Summary: Chemosensory receptor genes play an important role in insect chemodetection, but the understanding of these genes in Coleoptera is limited. In this study, the chemosensory receptor genes of the red-necked longicorn beetle were analyzed, and their functions in pheromone sensing and taste perception were identified.
Article
Multidisciplinary Sciences
Sheng Gao, Shu Jia, Xutao Fan, Chengcan Gao, Qingwei Li, Yuxue Wu, Chunyang Meng
Summary: This study identified and validated the potential role of the gene CD63 as a therapeutic target for intervertebral disc degeneration (IDD) through the integration of gene datasets and molecular mechanism analysis.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Hannah Collden, Maria E. Nilsson, Anna-Karin Norlen, Andreas Landin, Sara H. Windahl, Jianyao Wu, Karin L. Gustafsson, Matti Poutanen, Henrik Ryberg, Liesbeth Vandenput, Claes Ohlsson
Summary: This study established a comprehensive atlas of sex hormone concentrations in tissues and serum of male mice, revealing the distribution characteristics of sex hormones. Brain sex hormone levels are well correlated with serum levels, and white adipose tissue serves as a major reservoir of sex hormones in mice. Progesterone is the most abundant sex hormone in castrated mice.
Review
Biochemistry & Molecular Biology
Atsushi Hattori, Maki Fukami
Summary: This review article discusses the relationship between pathogenic variants of three nuclear receptor genes (NR5A1, NR0B1, and NR2F2) and disorders of sex development (DSD). NR5A1 variants are associated with 46,XY DSD and 46,XX testicular/ovotesticular DSD. NR0B1 and NR2F2 mutations are also implicated in DSD, but their exact roles are still unclear. Understanding these nuclear receptors provides new insights into the molecular networks involved in human fetal gonadal development.
Article
Plant Sciences
Hassan S. Al-Zahrani, Tarek A. A. Moussa, Hameed Alsamadany, Rehab M. Hafez, Michael P. Fuller
Summary: This study conducted a comparative analysis of the small GTPase superfamily in the Solanum lycopersicum super strain B and leguminous/non-leguminous species, showing variations in the number and composition of subfamily members. The expression of Rab and Arf proteins differed in roots and aerial tissues among different plants. The presence of diverse functions within GTPase superfamily members was observed, indicating potential alterations in function across different plants.
Review
Marine & Freshwater Biology
Minghui Li, Shengfei Dai, Xingyong Liu, Hesheng Xiao, Deshou Wang
Summary: Reverse genetics approaches, especially CRISPR/Cas9, are essential for understanding complex biological processes and genetic engineering. Nile tilapia, with its XX/XY sex determination system, serves as a valuable model for studying fish sex determination and reproduction. The detailed protocol provided in this study will facilitate the application of CRISPR/Cas9 in tilapia research and aquaculture studies.
Article
Biochemistry & Molecular Biology
Wenjing Tao, Luohao Xu, Lin Zhao, Zexian Zhu, Xin Wu, Qianwen Min, Deshou Wang, Qi Zhou
Summary: Researchers have reported the newly produced genomes of Nile tilapia and blue tilapia, providing detailed insights into their sex chromosome turnover mechanisms and sex-determining pathways, which are crucial for the development of genetic sex control in aquaculture. The study suggests that unequal distribution of repeat content may impact the local recombination rate, making some chromosomes more likely to become sex chromosomes.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Fisheries
Xin Wu, Lin Zhao, Zheng Fan, Baoyue Lu, Jinlin Chen, Dejie Tan, Dongneng Jiang, Wenjing Tao, Deshou Wang
Summary: The study bred WW super-females, identified structural variations in the sex determining region of blue tilapia, designed and screened three sex-linked markers, and successfully established a genetic sex identification technique. This is significant for studying sex determination genes, understanding sex chromosome evolution, and the transition of sex determination systems.
Review
Biochemistry & Molecular Biology
Wenjing Tao, Matthew A. Conte, Deshou Wang, Thomas D. Kocher
Summary: Patterns of gene network structure may play a crucial role in regulating sex chromosome replacement.
Article
Biochemistry & Molecular Biology
Matthew A. Conte, Frances E. Clark, Reade B. Roberts, Luohao Xu, Wenjing Tao, Qi Zhou, Deshou Wang, Thomas D. Kocher
Summary: This study investigates the evolution of a giant sex chromosome in cichlid fish, finding that it consists of three distinct regions and contains unprecedented amounts of endogenous retroviral elements, immunoglobulin genes, and long non-coding RNAs. Comparative analysis across 69 teleost genomes supports the hypothesis that the giant sex chromosome originated from the fusion of a B chromosome.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Reproductive Biology
Xiangyan Dai, Xinkai Cheng, Jianfei Huang, Yanping Gao, Deshou Wang, Zhi Feng, Gang Zhai, Qiyong Lou, Jiangyan He, Zhijian Wang, Zhan Yin
Summary: Our study demonstrates the crucial role of Rbm46 in zebrafish reproduction, showing that disruption of Rbm46 leads to masculinization and infertility by impairing germ cell progression through meiosis prophase I. Rbm46 may be involved in posttranscriptional regulation of key genes essential for germ cell development during gametogenesis.
BIOLOGY OF REPRODUCTION
(2021)
Review
Endocrinology & Metabolism
Linyan Zhou, Minghui Li, Deshou Wang
Summary: The involvement of sex steroids in sex determination and differentiation in non-mammalian vertebrates, especially in fish, is relatively conserved. Advances in genome sequencing and editing have led to significant progress in understanding steroidogenic pathways and hormonal regulation of sex determination in fish. Gene editing studies challenge traditional views on the roles of estrogen and androgen in ovarian and testicular development, and highlight the critical role of estrogen in female sex determination.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lu Li, You Wu, Chenhua Zhao, Yiyang Miao, Jing Cai, Lingyun Song, Jing Wei, Tapas Chakraborty, Limin Wu, Deshou Wang, Linyan Zhou
Summary: The StAR2 gene plays a critical role in testicular development, spermatogenesis, and spermiation in tilapia by regulating androgen production. However, it may not be essential and could be compensated for in the absence of StAR2.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Xingyong Liu, Shengfei Dai, Jiahong Wu, Xueyan Wei, Xin Zhou, Mimi Chen, Dejie Tan, Deyong Pu, Minghui Li, Deshou Wang
Summary: The study analyzed the expression and functions of amh duplicates in Nile tilapia. It was found that amhy is expressed earlier and at a higher level during sex determination, and it determines sex by repressing the transcription of cyp19a1a. Mutations in amhy lead to sex reversal and altered germ cell proliferation.
Article
Evolutionary Biology
Chenxu Wang, Jia Xu, Thomas D. Kocher, Minghui Li, Deshou Wang
Summary: In this study, researchers identified two pmel genes in Nile tilapia and demonstrated their importance in pigmentation. Homozygous mutation of pmela resulted in a yellowish body color with weak vertical bars, while mutation of pmelb had a milder effect. Double mutation of both genes led to a decrease in melanophores and an increase in xanthophores, resulting in a golden body color. The findings provide new strategies for breeding golden tilapia and offer a new model for studying pmel function in vertebrates.
JOURNAL OF HEREDITY
(2022)
Article
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
Summary: Teleosts are important models for studying sex chromosomes and sex-determining genes. In this study, a high-contiguity chromosome-level genome assembly on a YY southern catfish was generated using Nanopore and Hi-C technologies. The study identified a candidate sex-determining gene in the sex-determining region and confirmed the role of amhr2y in male sex determination.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Chunmei Xu, Miao Yu, Qingqing Zhang, Zhisheng Ma, Kang Du, Huiqin You, Jing Wei, Deshou Wang, Wenjing Tao
Summary: In this study, the BRD gene family was identified and characterized in Nile tilapia, revealing sexually dimorphic expression patterns and their critical role in spermatogenesis. The findings provide insights into the understanding of sex differentiation and gonadal development in teleosts.
Correction
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xingyong Liu, Li Zhou, Wenbo Li, Jiahong Wu, Deshou Wang
Summary: In this study, it was found that GATA2 plays a critical role in lens morphogenesis in Nile tilapia. Mutations in the gata2a gene led to degeneration and apoptosis of lens fiber cells, causing progressive microphthalmia and blindness in adulthood. Transcriptome analysis revealed significant changes in the expression levels of genes encoding crystallin and genes involved in visual perception and metal ion binding after gata2a mutation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Zeming Zhang, Jie Wang, Jianeng Li, Xiang Liu, Lei Liu, Changle Zhao, Wenjing Tao, Deshou Wang, Jing Wei
Summary: In this study, an integrated CRISPR/Cas9 system was established for gene knock-out and knock-in at a specific site in medaka. The system is simple, cost-effective, and efficient. The results showed that pCas9-mU6sgRNA driven by endogenous U6 promoter achieved high gene editing efficiency in medaka cultured cells, but not by exogenous U6 promoters. This study provides insights into other fish genome editing and promotes gene functional analysis.
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.