Article
Food Science & Technology
Yushi Fujimura, Mariko Shimura, Hiroshi Nagai, Naoko Hamada-Sato
Summary: This study demonstrated that abalone viscera fermented by lactic acid bacteria have the ability to inhibit angiotensin-converting enzyme (ACE) and suppress blood pressure increases, with homarine identified as the active compound. Therefore, fermented abalone viscera have the potential to be used as functional food materials to manage blood pressure.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biotechnology & Applied Microbiology
Sizhe Qiu, Hong Zeng, Zhijie Yang, Wei-Lian Hung, Bei Wang, Aidong Yang
Summary: In this study, a dynamic metagenome-scale metabolic model was built to investigate metabolic interactions between Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus in yogurt fermentation. The model, which integrated constrained proteome allocation, was validated by comparing predicted bacterial growth, lactose consumption, and lactic acid production with experimental data. The dynamic simulation revealed the mutual dependence of S. thermophilus and L. d. bulgaricus during yogurt fermentation. This dynamic metabolic model of the yogurt bacterial community provides a foundation for computer-aided process design and control of fermented dairy product production.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Nutrition & Dietetics
Jie Yang, Yue Sun, Tengqi Gao, Yue Wu, Hao Sun, Qingzheng Zhu, Chunsheng Liu, Chuang Zhou, Yongbin Han, Yang Tao
Summary: This study shows that lactic acid bacteria can effectively ferment apple juice, resulting in increased viable cell counts, lactic acid content, and improved antioxidant and antibacterial capacities. After storage, the fermented apple juice still retains its antibacterial and in vitro antioxidant activities.
FRONTIERS IN NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Chong Chen, Shuo Zu, Dongxing Zhang, Zelin Zhao, Yalu Ji, Hengyu Xi, Xiaofeng Shan, Aidong Qian, Wenyu Han, Jingmin Gu
Summary: This study demonstrates the potential of recombinant Lactobacillus casei strains as oral vaccines against Aeromonas veronii infection in Cyprinus carpio. The recombinant strains were able to induce immune responses and enhance immune activity in the fish, leading to increased survival rates against infection.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Askar Kondybayev, Gaukhar Konuspayeva, Caroline Strub, Gerard Loiseau, Christian Mestres, Joel Grabulos, Marie Manzano, Shynar Akhmetsadykova, Nawel Achir
Summary: The growth characteristics of two strains of lactic acid bacteria (LAB), Lacticaseibacillus casei and Lactobacillus kefiri, isolated from qymyz, were studied and modeled. The study found that fermentation conditions have a significant impact on the population of LAB and the production of acid and ethanol, and controlling pH, temperature, and substrates can improve the production of qymyz.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Yong Jun Goh, Rodolphe Barrangou
Summary: This study developed a CRISPR-based editing system for genome manipulations in three Lactobacillus species, demonstrating its efficacy in two distantly related Lactobacillus species. This versatile Cas9 system facilitates genome engineering compared to conventional gene replacement systems and is a valuable gene editing modality in species without native CRISPR-Cas systems. Overall, this portable tool expands the genome editing toolbox for studying health-promoting mechanisms and engineering beneficial microbes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Environmental Sciences
Min Gan, Jian Hu, Kai Wan, Xiangxiang Liu, Peirong Chen, Rui Zeng, Fuhua Wang, Yarong Zhao
Summary: In this study, five strains of lactic acid bacteria were identified for their ability to remove zearalenone (ZEN) from food and feed. These bacteria not only reduced the toxicity of ZEN, but also bonded with it through interactions with cell wall compounds. The findings suggest that these strains have the potential to be used as detoxification agents for ZEN removal.
Article
Biotechnology & Applied Microbiology
Luis J. Bastarrachea, David W. Britt, Robert E. Ward, Ali Demirci
Summary: The modification of polypropylene with chitosan was found to enhance the generation of Lactobacillus casei biofilms and their lactic acid production. Biofilms grown in rich media produced significantly more lactic acid, while the chitosan-modified polypropylene harbored more cells compared to the control. The deposition of nutrients and biomass on the chitosan-modified polypropylene was confirmed to be higher, with thicker biofilms observed when grown in rich media.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Ilenys M. Perez-Diaz, Suzanne D. Johanningsmeier, Kartheek Anekella, Christian G. Pagan-Medina, Lesley Mendez-Sandoval, Consuelo Arellano, Robert Price, Katheryne Daughtry, Michelle Borges, Chloe Bream, Lauren Connelly, Susan E. Dieck, Meredith T. Levi, Erin K. McMurtrie, Rickey E. Smith, Jeannette C. Theora, Paige Wendland, Francisco Gomez-Rodriguez, Francisco Noe Arroyo-Lopez
Summary: This study defined the genotypes of Lactobacillus plantarum and Lactobacillus pentosus in industrial-scale cucumber fermentations using rep-PCR-(GTG)5, and evaluated their ability to ferment cucumbers under various conditions. It was found that L. pentosus strains were more efficient in cucumber juice fermentation compared to most L. plantarum strains. This research identified genetically diverse L. pentosus strains and one L. plantarum strain as candidates for starter cultures in commercial cucumber fermentations.
Article
Biotechnology & Applied Microbiology
Paola Monteiro de Oliveira, Larissa Provasi Santos, Luciana Fontes Coelho, Paulo Marcelo Avila Neto, Daiane Cristina Sass, Jonas Contiero
Summary: This study focuses on obtaining L(+)-lactic acid from sucrose using Lactobacillus casei Ke11 strain through different feeding strategies, which increases lactic acid production and productivity, while avoiding cellular stress caused by high osmotic pressure. The purification of the acid using charcoal and celite, followed by a cation exchange column, proved to be highly efficient, allowing for a high yield of lactic acid and removal of sugars and proteins. This study shows potential for large-scale fermentation of L. casei Ke11 for production of l-lactic acid.
FERMENTATION-BASEL
(2021)
Article
Multidisciplinary Sciences
Babak Haghshenas, Amir Kiani, Saeideh Mansoori, Ehsan Mohammadi-noori, Yousef Nami
Summary: The use of medicinal plants for disease treatment and prevention is gaining attention among researchers. This study focused on the probiotic properties and antibacterial and antifungal activity of silymarin-enriched Lactobacillus bacteria against pathogenic bacteria and Aspergillus flavus. Five strains of bacteria were identified as potential probiotics based on their viability and various characteristics. These strains demonstrated effective antibacterial and antifungal activity against multiple pathogens and A. flavus. Furthermore, the combination of silymarin and probiotics showed a significantly stronger antifungal effect compared to the control group or probiotics alone. The presence of silymarin extract in methanol, DMSO, and PEG enhanced the antagonistic activity of the probiotic strains.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Camila Ramao Contessa, Nathieli Bastos de Souza, Guilherme Battu Goncalo, Catarina Motta de Moura, Gabriela Silveira da Rosa, Caroline Costa Moraes
Summary: A bioplastic antibacterial film incorporating bacteriocin was developed for commercial curd cheese, which demonstrated microbial reduction and contributed to reducing contamination of food products with high initial microbial load.
Article
Microbiology
Zongjie Li, Yuhao Li, Changguang Xiao, Zujie Yan, Ruyi Pan, Yun Gao, Beibei Li, Jianchao Wei, Yafeng Qiu, Ke Liu, Donghua Shao, Zhiyong Ma
Summary: This research sequenced and analyzed the genome of L. sakei JD10 strain isolated from porcine lung. The genome analysis revealed genes responsible for the production of inhibitory substances, carbohydrate degradation enzymes, and short chain fatty acids, as well as their regulatory effects on bacterial phagocytosis. Bacteriocin-producing genes and CRISPR-Cas system related genes were also predicted in the genome. Overall, L. sakei JD10 showed potential probiotic traits in fighting pathogenic infection and regulating host immune function.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Kathy G. Arriola, Diwakar Vyas, Donghyeon Kim, Mariele C. N. Agarussi, Vanessa P. Silva, Milton Flores, Yun Jiang, Xue Yanlin, Andres A. Pech-Cervantes, Luiz F. Ferraretto, Adegbola T. Adesogan
Summary: The study aimed to determine the effect of inoculation with Lactobacillus hilgardii and Lactobacillus buchneri on sorghum and corn silage. Results showed that inoculating with LB or LH separately improved aerobic stability, while the combination of LB and LH prevented increases in DM losses caused by their separate use.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Saowalak Adunphatcharaphon, Awanwee Petchkongkaew, Wonnop Visessanguan
Summary: This study evaluated the ZEA removal capacity of plant-derived lactic acid bacteria (LAB) and found that Lactobacillus plantarum BCC 47723 exhibited the highest removal efficiency. Physical and chemical treatments affected the bacterial cell structure and morphology, influencing the adsorption and removal of ZEA. The findings suggest that hydrophobicity is the main mechanism for ZEA removal by L. plantarum BCC 47723, and heat treatment can enhance its efficiency for ZEA reduction, making it a potential organic adsorbent for ZEA reduction in food and feedstuff.
Review
Biotechnology & Applied Microbiology
Wei Song, Xiulai Chen, Jing Wu, Jianzhong Xu, Weiguo Zhang, Jia Liu, Jian Chen, Liming Liu
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biotechnology & Applied Microbiology
Rui Yao, Pei Zhou, Chengjin Wu, Liming Liu, Jing Wu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Guoxing Zhu, Nannan Yin, Qiuling Luo, Jia Liu, Xiulai Chen, Liming Liu, Jianrong Wu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Jian-Zhong Xu, Hao-Zhe Ruan, Hai-Bo Yu, Li-Ming Liu, Weiguo Zhang
MICROBIAL CELL FACTORIES
(2020)
Article
Chemistry, Physical
Liang Guo, Wenwen Diao, Cong Gao, Guipeng Hu, Qiang Ding, Chao Ye, Xiulai Chen, Jia Liu, Liming Liu
Article
Biotechnology & Applied Microbiology
Xiulai Chen, Juyang Yi, Wei Song, Jia Liu, Qiuling Luo, Liming Liu
Summary: Chassis engineering was used to enhance enzymatic production of Hyp in Escherichia coli, leading to significant improvements in Hyp titre, conversion rate and productivity. Rewiring the TCA cycle and l-proline degradation pathway increased alpha-ketoglutarate supply, while fine-tuning heterologous haemoglobin expression improved oxygen transfer, resulting in a more efficient method for industrial production of Hyp.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiulai Chen, Juyang Yi, Jia Liu, Qiuling Luo, Liming Liu
Summary: This study presents a green and efficient production process for Hyp by overexpressing DsP4H enzyme and optimizing culture parameters, achieving high yields and conversion rates.
MICROBIAL BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Xu Tan, Wei Song, Xiulai Chen, Liming Liu, Jing Wu
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Qiang Ding, Wenwen Diao, Cong Gao, Xiulai Chen, Liming Liu
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biotechnology & Applied Microbiology
Jianshen Hou, Cong Gao, Liang Guo, Jens Nielsen, Qiang Ding, Wenxiu Tang, Guipeng Hu, Xiulai Chen, Liming Liu
METABOLIC ENGINEERING
(2020)
Review
Biochemical Research Methods
Liang Guo, Zixuan Pang, Cong Gao, Xiulai Chen, Liming Liu
CURRENT OPINION IN BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Nan Xu, Qiyuan Yang, Xiaojing Yang, Mingqi Wang, Minliang Guo
Summary: This study utilized a genome-scale metabolic model to investigate the metabolic characteristics of Agrobacterium tumefaciens and found differences in metabolic activity in different ecological niches. By integrating transcriptome data under tumor conditions, significant changes in metabolic pathways were identified.
MOLECULAR PLANT PATHOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Hui Liu, Yanli Qi, Pei Zhou, Chao Ye, Cong Gao, Xiulai Chen, Liming Liu
Summary: This review proposes a strategy of microbial physiological engineering (MPE) to improve the efficiency of microbial cell factories, focusing on enhancing substrate utilization, growth performance, stress tolerance, and product export capacity.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yang Li, Shujie Yang, Danlei Ma, Wei Song, Cong Gao, Liming Liu, Xiulai Chen
Summary: Organic acids, as building block compounds, are widely used in various industries with chemical synthesis being the primary method. Microbial cell factories offer a promising approach for sustainable organic acid production but face challenges in cellular metabolism regulation. Metabolic engineering strategies have been developed to reprogram microbial cell factories for higher productivity of organic acids by improving enzyme efficiency, balancing biosynthetic pathways, enhancing metabolic flux, optimizing the metabolic network, and broadening substrate utilization.
NATURAL PRODUCT REPORTS
(2021)
Article
Genetics & Heredity
Nan Xu, Mingqi Wang, Xiaojing Yang, Yujuan Xu, Minliang Guo
MICROBIAL GENOMICS
(2020)
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.
