Article
Biotechnology & Applied Microbiology
Jing Chen, Xiuli Chen, Xin Huang, Guanghua Huang, Zexia Gao, Weimin Wang, Han Liu
Summary: Intermuscular bone (IB) is a hard-boned spicule found in lower teleost, negatively impacting the palatability and economic value of blunt snout bream (Megalobrama amblycephala). Through molecular analysis, key signaling pathways related to IB formation have been identified, indicating a possible involvement of intramembranous ossification in the development process.
Article
Fisheries
Xiao-Cheng Liu, Min Huang, Xiao-Ping Huang, Jun-Feng Guan, Xiang-Fei Li, Di-Zhi Xie, Chao Xu
Summary: This study cloned and characterized the NLRP3 gene from Megalobrama amblycephala and investigated its function in high-glucose-induced inflammation. The results showed that NLRP3 played a role in the regulation of HG-induced inflammation, and high-leucine levels could aggravate this inflammation via NLRP3 activation in hepatocytes of M. amblycephala.
Article
Biochemistry & Molecular Biology
Hujun Cui, Hong Li, Minying Zhang, Hongping Li, Xu Wang, Zirui Wang, Wei Zhai, Xiangning Chen, Hanliang Cheng, Jianhe Xu, Xiaoheng Zhao, Zhujin Ding
Summary: CD68 is a highly glycosylated transmembrane glycoprotein involved in immune processes in fish. In this study, the expression patterns and evolutionary characteristics of Megalobrama amblycephala CD68 (MaCD68) were analyzed. MaCD68 was highly expressed in different tissues and played a role in the host's innate immune response against bacterial infection. Moreover, MaCD68 may serve as a macrophage marker and be involved in immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Qijun Wang, Xiaoheng Zhao, Yunlong Liu, Juan Zheng, Hujun Cui, Haotong Wang, Houxu Ding, Hong Liu, Zhujin Ding
Summary: This study identified two hemoglobin subunits in Megalobrama amblycephala and analyzed their expression patterns and antibacterial activities. The findings revealed different expression patterns of these subunits in various tissues and demonstrated their excellent antimicrobial activities.
Article
Biochemistry & Molecular Biology
Jose Carlos Montanes, Marta Huertas, Xavier Messeguer, M. Mar Alba
Summary: The formation of new genes can occur through gene duplication or de novo formation from noncoding sequences. Both mechanisms show similarities in terms of low sequence constraints in the initial evolutionary stages, high turnover rates at the species level, and comparable persistence rates in deeper branches. Additionally, de novo proteins tend to have an excess of substitutions between charged amino acids, leading to rapid loss of their initial highly basic character.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Cell Biology
Yulong Chen, Shiming Wan, Qing Li, Xiaoru Dong, Jinghan Diao, Qing Liao, Gui-Ying Wang, Ze-Xia Gao
Summary: Our study utilized SMRT technology to characterize the transcriptome of blunt snout bream and identified differentially expressed miRNAs, mRNAs, and lncRNAs between two intermuscular bone growth stages. KEGG analysis highlighted key regulatory genes in the MAPK/p53 and ECM-receptor signaling pathways. Subsequent experiments revealed core regulatory factors affecting cell apoptosis and mineralization activity in intermuscular bones.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Fisheries
Hujun Cui, Xiaoxue Shen, Yancui Zheng, Peng Guo, Zhaotian Gu, Yanan Gao, Xiaoheng Zhao, Hanliang Cheng, Jianhe Xu, Xiangning Chen, Zhujin Ding
Summary: In this study, the MaCD209 gene of Megalobrama amblycephala was cloned and characterized. The gene was found to be highly expressed in the liver and heart and was significantly induced upon Aeromonas hydrophila infection. Recombinant MaCD209 protein showed excellent bacterial binding and agglutinative activities.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Plant Sciences
Licao Cui, Hao Cheng, Zhe Yang, Chuan Xia, Lichao Zhang, Xiuying Kong
Summary: Wheat is a staple food crop that provides 20% of total human calorie consumption. Duplicated genes in wheat evolved faster with shorter gene lengths, higher codon usage bias, lower expression levels, and higher tissue specificity. Duplicated genes have important biological functions, particularly in response to environmental stresses, and different duplication modes exhibit asymmetrical evolutionary patterns. This study lays the foundation for future functional studies on wheat.
Article
Fisheries
Shiyou Chen, Yan Lin, Linghong Miao, Wenjing Pan, Wenqiang Jiang, Linjie Qian, Jingyuan Hao, Bingwen Xi, Bo Liu, Xianping Ge
Summary: The study investigated the effects of ferulic acid (FA) on oxidative stress and inflammatory responses in blunt snout bream by creating a model of oxidative stress and inflammation through lipopolysaccharide (LPS) injection. FA treatment showed significant reductions in apoptosis rates, improvements in liver structure, enhanced antioxidant enzyme activities, and decreased pro-inflammatory cytokine levels, highlighting its potential as an effective antioxidant and anti-inflammatory agent in fish feed additives.
Article
Biochemistry & Molecular Biology
Colin J. Shew, Paulina Carmona-Mora, Daniela C. Soto, Mira Mastoras, Elizabeth Roberts, Joseph Rosas, Dhriti Jagannathan, Gulhan Kaya, Henriette O'Geen, Megan Y. Dennis
Summary: Evidence shows that in humans, paralogous segmental duplication genes exhibit different expression patterns, possibly due to relaxed selection or neofunctionalization. While ancestral paralogs show greater expression conservation with chimpanzee orthologs, certain derived paralogs may retain or supplant ancestral functions.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Han Liu, Chunhai Chen, Maolin Lv, Ning Liu, Yafei Hu, Hailin Zhang, Erik D. Enbody, Zexia Gao, Leif Andersson, Weimin Wang
Summary: This study reports a major OR expansion in a freshwater herbivorous fish, Megalobrama amblycephala, and evaluates the possible contribution of OR expansion to habitat and feeding specialization by comparing the OR repertoire in 28 phylogenetically and ecologically diverse teleosts. The research found a strong association between duplications of beta and epsilon ORs and freshwater habitat, with the expanded beta and epsilon ORs highly expressed in the olfactory epithelium of Megalobrama amblycephala.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biotechnology & Applied Microbiology
Wuhui Li, Shi Wang, Jie Hu, Chenchen Tang, Chang Wu, Junmei Liu, Li Ren, Chengfei Sun, Junjian Dong, Shaojun Liu, Xing Ye
Summary: The study found that diet drives phenotypic and genetic variation in hybrid fish, and the asymmetric expression of homoeologous genes may play key roles in dietary adaptation. Subgenome expression dominance may contribute to uncovering the mechanistic basis of heterosis and also provide perspectives for fish genetic breeding and application.
Article
Immunology
Aotian Ouyang, Huabing Wang, Jianguo Su, Xiaoling Liu
Summary: COS6 enters blunt snout bream macrophages through MaMR CTLD4-8, suppressing the expression of inflammatory genes while other receptors are involved in immune regulation. MaMR CTLD4-8 cooperates with TLR4 to regulate the pro-inflammatory response caused by COS6 in macrophages of blunt snout bream.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Jixiu Wang, Hang Luo, Qianhui Sun, Huanling Wang, Hong Liu
Summary: The study investigated the expression pattern of the beta(2)m gene in Megalobrama amblycephala and found significant increases in expression levels after infection with Aeromonas hydrophila. Four SNPs were identified in the beta(2)m gene, with one showing significant differences in genotype and allele frequencies between resistant and susceptible groups. These results provide theoretical support for further functional studies of the beta(2)m gene and breeding of disease-resistant strains of M. amblycephala.
Article
Fisheries
Shujuan Wang, Zhipeng Tai, Qianhui Sun, Jixiu Wang, Huanling Wang, Zexia Gao, Hong Liu
Summary: This study identified two Akirin genes, Akirin1 and Akirin2, in the fish species Megalobrama amblycephala. The results showed that Akirin2 played a key role in regulating the immune response to bacterial infection in fish. It was found that Akirin2 could induce the transcription of downstream antimicrobial peptides through the NF-kappa B signaling pathway, enhancing the fish's immunity to Aeromonas hydrophila infection.
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.